Microextraction and Determination of Diclofenac in Biological Samples using Hollow Fiber Liquid Phase Microextraction Technique Coupled with HPLC-UV

Subject Areas : Polymer

Mohammad Reza  Saadat ¹ (Pharmaceutical Sciences Research Center, Pharmaceutical Sciences Branch , Islamic Azad University, Tehran, Iran)
Mahnaz  Qomi ² (2,4Active Pharmaceutical Ingredients Research Center, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran)
Sahel  Emadzadeh ³ (Pharmaceutical Sciences Research Center, Pharmaceutical Sciences Branch , Islamic Azad University, Tehran, Iran)
Marjan  Gholghasemi ⁴ (Active Pharmaceutical Ingredients Research Center, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran)

Received: 2017-12-24 Accepted : 2017-12-24 Published : 2018-01-01

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Abstract :

In this study, hollow fiber liquid phase microextraction (HF-LPME) coupled with highperformanceliquid chromatography (HPLC) with UV detection was applied for preconcentrationand determination of Diclofenac sodium in biological fluids. Parameters affecting the extractionprocess including pH of donor phase and acceptor phase, type of extraction solvent, stirring rate,extraction time, and salt addition were studied and optimized. Under the optimized conditionsdonor phase pH=3, acceptor phase pH=11.7, extraction solvent n-octanol, stirring rate =750 rpm,extraction time=27 min and without the addition of salt, enrichment factors up to 170 wereachieved and the relative standard deviation (RSD %) of the method was within the range of 2-3%. The method was successfully applied to determine the concentration of diclofenac sodium inthe biological samples and satisfactory results were obtained.