An Investigation of the Charge Effects on the Uptake and Release Capacity of Mesoporous Silica Nanoparticles as Vehicles for Short Oligonucleotides

Subject Areas : Polymer

Rafatosadat  Badihi ¹ (Faculty of Chemistry, North Tehran Branch, Islamic Azad University, Hakimiyeh, Tehran, Iran)
Ali  Mahmoudi ² (Faculty of Chemistry, North Tehran Branch, Islamic Azad University, Hakimiyeh, Tehran, Iran)
Mohammad Reza  Sazegar ³ (Faculty of Chemistry, North Tehran Branch, Islamic Azad University, Hakimiyeh, Tehran, Iran)
Khodadad  Nazari ⁴ (Future Bioenergy Solutions Inc., 369-901 3rd Street West, North Vancouver BC, V7P 3P9, Canada)

Received: 2023-12-16 Accepted : 2023-12-16 Published : 2024-01-01

Keywords:

Abstract :

An easy and cost-effective sol-gel procedure is described for the synthesis and co-modification of mesoporous silica nanoparticles (MSNs) with various metal ions and positive-charge inducing polymers including PEI and PEG, for the construction of a reliable gene delivery vehicle. All of the samples (denoted as PEI-MSN, M-MSN, PEI-M-MSN, and PEG-PEI-MSN, where, M= Fe2+, Fe3+, Co2+, Zn2+, Al3+), were unambiguously characterized by conventional techniques including FTIR, XRD, SEM, and BET. The adsorption capacity of siRNA was found to be more related to the zeta potential of the samples than their specific surface areas. The best adsorption result was obtained by using PEI-Fe(III)-MSN (48.8 µg of siRNA per mg of support). The release of siRNA from PEIFe(III)-MSN, was also good (91.5%), but a burst release profile was observed. Interestingly, when a layer of PEG was used as a co-modifier, a sustained release profile was achieved, and meanwhile, the released amount of siRNA was improved (93.2%). The whole combination showed noconsiderable cytotoxicity according to the MTT test.

References:

1. Yin H, Kanasty RL, Eltoukhy AA, Vegas AJ, Dorkin JR, Anderson DG. Non-viral vectors for gene-based therapy. Nature Reviews Genetics. 2014;15(8):541-55.


2. Rosenberg SA, Aebersold P, Cornetta K, Kasid A, Morgan RA, Moen R, Karson EM, Lotze MT, Yang JC, Topalian SL, Merino MJ. Gene transfer into humans—immunotherapy of patients with advanced melanoma, using tumor-infiltrating lymphocytes modified by retroviral gene transduction. New England Journal of Medicine. 1990;323(9):570-8.


3. Wittrup A, Lieberman J. Knocking down disease: a progress report on siRNA therapeutics. Nature Reviews Genetics. 2015;16(9):543-52.


4. Wang KE, Chen W, Zhang Z, Deng Y, Lian JQ, Du P, Wei D, Zhang Y, Sun XX, Gong L, Yang X. CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells. Signal transduction and targeted therapy. 2020;5(1):283.


5. Zeng H, Little HC, Tiambeng TN, Williams GA, Guan Z. Multifunctional dendronized peptide polymer platform for safe and effective siRNA delivery. Journal of the American Chemical Society. 2013;135(13):4962-5.


6. Zhang Y, Ren K, Zhang X, Chao Z, Yang Y, Ye D, Dai Z, Liu Y, Ju H. Photo-tearable tape close-wrapped upconversion nanocapsules for near-infrared modulated efficient siRNA delivery and therapy. Biomaterials. 2018;163:55-66.


7. Van Bruggen C, Hexum JK, Tan Z, Dalal RJ, Reineke TM. Nonviral gene delivery with cationic glycopolymers. Accounts of Chemical Research. 2019;52(5):1347-58.


8. Li Z, Zhang Y, Feng N. Mesoporous silica nanoparticles: synthesis, classification, drug loading, pharmacokinetics, biocompatibility, and application in drug delivery. Expert opinion on drug delivery. 2019;16(3):219-37.


9. Zhou Y, Quan G, Wu Q, Zhang X, Niu B, Wu B, Huang Y, Pan X, Wu C. Mesoporous silica nanoparticles for drug and gene delivery. Acta pharmaceutica sinica B. 2018;8(2):165-77.


10. Shao D, Lu MM, Zhao YW, Zhang F, Tan YF, Zheng X, Pan Y, Xiao XA, Wang Z, Dong WF, Li J. The shape effect of magnetic mesoporous silica nanoparticles on endocytosis, biocompatibility and biodistribution. Acta biomaterialia. 2017;49:531-40.


11. Hajiagha NG, Mahmoudi A, Sazegar MR, Pouramini MM. Synthesis of cobalt-modified MSN as a model enzyme: Evaluation of the peroxidatic performance. Microporous and Mesoporous Materials. 2019;274:43-53.


12. Kneuer C, Sameti M, Haltner EG, Schiestel T, Schirra H, Schmidt H, Lehr CM. Silica nanoparticles modified with aminosilanes as carriers for plasmid DNA. International journal of pharmaceutics. 2000;196(2):257-61.


13. Badihi R, Mahmoudi A, Sazegar MR, Nazari K. A study on co-modification of MSNs with some transition metals and polyethyleneimine (PEI) as a versatile strategy for efficient delivery of short oligonucleotides. Chemical Papers. 2022;76(11):7023-35.


14. Slowing II, Vivero-Escoto JL, Wu CW, Lin VS. Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carriers. Advanced drug delivery reviews. 2008;60(11):1278-88.


15. Teo PY, Cheng W, Hedrick JL, Yang YY. Co-delivery of drugs and plasmid DNA for cancer therapy. Advanced drug delivery reviews. 2016;98:41-63.


16. Xu W, He W, Du Z, Zhu L, Huang K, Lu Y, Luo Y. Functional nucleic acid nanomaterials: development, properties, and applications. Angewandte Chemie International Edition. 2021;60(13):6890-918.


17. Nakamura T, Mizutani M, Nozaki H, Suzuki N, Yano K. Formation mechanism for monodispersed mesoporous silica spheres and its application to the synthesis of core/shell particles. The Journal of Physical Chemistry C. 2007;111(3):1093-100.


18. Ma Z, Guan Y, Liu H. Superparamagnetic silica nanoparticles with immobilized metal affinity ligands for protein adsorption. Journal of Magnetism and Magnetic Materials. 2006;301(2):469-77.


19. von Baeckmann C, Kählig H, Lindén M, Kleitz F. On the importance of the linking chemistry for the PEGylation of mesoporous silica nanoparticles. Journal of Colloid and Interface Science. 2021;589:453-61.


20. Mardani F, Khorshidi A, Gholampoor S. Sulfonated Caspian Sea Sand: A Promising Heterogeneous Solid Acid Catalyst in Comparison with–SO3H Functionalized NiFe2O4@ SiO2@ KIT‐6. ChemistrySelect. 2019;4(27):8015-20.


21. Akbarzadeh M, Oskuee RK, Gholami L, Mahmoudi A, Malaekeh-Nikouei B. BR2 cell penetrating peptide improved the transfection efficiency of modified polyethyleneimine. Journal of Drug Delivery Science and Technology. 2019;53:101154.


22. Lindén JB, Larsson M, Kaur S, Nosrati A, Nydén M. Glutaraldehyde‐crosslinking for improved copper absorption selectivity and chemical stability of polyethyleneimine coatings. Journal of Applied Polymer Science. 2016;133(37).


23. Ganguly A, Ganguli AK. Anisotropic silica mesostructures for DNA encapsulation. Bulletin of Materials Science. 2013;36:329-32.


24. Zhang X, Zhang J, Quan G, Yang P, Pan X, Wu C. The serum-resistant transfection evaluation and long-term stability of gene delivery dry powder based on mesoporous silica nanoparticles and polyethyleneimine by freezing-drying. AAPS PharmSciTech. 2017;18:1536-43.


25. Kim H, Yuk SA, Dieterly AM, Kwon S, Park J, Meng F, Gadalla HH, Cadena MJ, Lyle LT, Yeo Y. Nanosac, a noncationic and soft polyphenol nanocapsule, enables systemic delivery of siRNA to solid tumors. Acs Nano. 2021;15(3):4576-93.


26. Andhariya JV, Jog R, Shen J, Choi S, Wang Y, Zou Y, Burgess DJ. In vitro-in vivo correlation of parenteral PLGA microspheres: Effect of variable burst release. Journal of Controlled Release. 2019;314:25-37.


27. Habra K, Morris RH, McArdle SE, Cave GW. Controlled release of carnosine from poly (lactic-co-glycolic acid) beads using nanomechanical magnetic trigger towards the treatment of glioblastoma. Nanoscale Advances. 2022;4(10):2242-9.


28. Yamada S, Chai Y, Tagaya M. PEG functionalization effect of silicate-containing hydroxyapatite particles on effective collagen fibrillation with hydration layer state change. Physical Chemistry Chemical Physics. 2022;24(11):6788-802.


29. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods. 1983;65(1-2):55-63.


30. Kanamala M, Palmer BD, Jamieson SM, Wilson WR, Wu Z. Dual pH-sensitive liposomes with low pH-triggered sheddable PEG for enhanced tumor-targeted drug delivery. Nanomedicine. 2019;14(15):1971-89.