Microwave-assisted Solid-phase(SPPS) and Solution-phase (SPS) Synthesis of Biological Dipeptide ((β-alanine-L histidine)

Subject Areas : Polymer

Maryam Khosravi ¹ , Rahmatollah Rahimi ² , Mahboubeh Rabbani ³ , Ebrahim Safavi ⁴

1 - Bionorganic Chemistry Research Laboratory, Chemistry Department, Iran University of Science and Technology, Narmak, Tehran, Iran
2 - Bionorganic Chemistry Research Laboratory, Chemistry Department, Iran University of Science and Technology, Narmak, Tehran, Iran
3 - Bionorganic Chemistry Research Laboratory, Chemistry Department, Iran University of Science and Technology, Narmak, Tehran, Iran
4 - World Society of the Anti-Aging Medicine and International Hormone Society, Niavaran,Tehran, Iran

Received: 2017-07-27 Accepted : 2017-07-27 Published : 2017-07-01

Keywords:

Abstract :

Peptides have shown Promising effect as pharmaceutics with the potential to treat a widevariety of diseases. Peptides are mostly synthesized by biological technology or chemicalmethods. Solution phasepeptide synthesis (SPS) and solid phase peptide synthesis (SPPS) aretwo major chemical techniques for peptidesproduction.In this research, the synthesis ofdipeptde(β-alanine-L-histidine)wasexaminedby the both SPS and SPPS methods. Thesolution phase synthesizeswasdonebyN-phthalic anhydride as a protective group andthe solidphase using standardtert-Butyloxycarbonyl (BOC) and Fluorenylmethyloxycarbonylchloride(Fmoc).Inthe both methods,themicrowave was assisted in different steps. Themolecular structure of the dipeptide(β-alanine-L-histidine) wasdefined using differentspectrometrymethods such as:UV-Vis, FT-IR, 1HNMR and LC-Massanalysis.