Assessment of some phenolic acids of artichoke callus under in vitro conditions. Mahsa Sanavi Joshaghani*, Azim Ghasemnezhad, Mahdi Alizadeh and Atana Tanuri
Subject Areas : Phytochemistry
Keywords: Callus, artichoke, explant, 2, 4-D, Media culture, phenolic acids,
Abstract :
Medicinal plants are the most important sources of life saving drugs for the majority of world's population. Artichoke is one of the oldest medicinal plants from asteraceae family. In the present study, caffeic acid and chlorogenic acid accumulation of different organs of artichoke callus were studied under in vitro conditions in MS, SH, and B5 media. The experiment was performed based on a completely randomized design with 3 replications. The artichoke seeds were transferred to the sterile Petri dishes containing filter paper immediately after disinfecting under sterile conditions. The obtained seedlings were transferred to half-strength MS medium. Only non-contaminated seedlings were used for preparation of root, leaf, and petiole explants. The SH, MS, and B5 media containing 0.8% agar, 3% sucrose, and 0, 0.5, 0.75, and 1 (mg/l) 2,4-D were used for callus induction. According to the results, caffeic acid accumulation was not found to be statistically different (α<0.05) among various media but maximum amount of chlorogenic acid accumulation between different media was recorded in SH medium containing 0.75 (mg/l) 2,4-D and with the leaf explant. In all culture media callus formation was related to the presence of 2.4,D. Otherwise, no callus formation was observed in hormone-free media.