Variation in steviol glycoside contents of stevia (Stevia rebaudiana) leaves under various leaf drying processes
Subject Areas : Phytochemistry
Mojtaba Karimi
1
(Shahrekord University)
Fatemeh Direkvand-moghadam
2
(Central Lab, Ramin Agricultural and Natural Resources University, Ahvaz, Iran.)
nafiseh Sheibani
3
(shahrekord university)
Keywords: infrared, Microwave, Stevioside, Rebaudioside A, Freeze-dry,
Abstract :
Stevia (stevia rebaudiana) leaves contain valuable sweet compounds, called steviol glycosides (SVglys), which the quantity and quality of these sweet compounds may be changed during the leaf drying process. The present study was conducted to survey the SVglys changes during different leaf drying methods applied for stevia leaves. The stevia leaves were similarly harvested before flowering and dried by different methods including oven (45 and 75 ˚C), shade, infrared, microwave, sun, and freeze-dry, and thereafter the SVglys were assessed. The results showed that the SVglys content and its compositions such as stevioside (Stev) and rebaudioside A (Reb A) were significantly affected by leaf drying methods. The highest content of SVglys and Stev was stabilized using infrared, microwave, sun, and freeze-dry methods while the other methods showed a lower content of SVglys and Reb A. The significant high content of Reb A was recorded by freeze-drying and sun methods. Our results conclusively showed that freeze-dry and sun drying methods were seen more effective than other drying methods in SVglys retention in leaves and recorded the lowest SVglys losses during the drying process.
Afshari, F., F. Nakhaei, S. Mosavi and M. Seghatoleslami, 2020. Evaluating the role of nutri-priming in improving PEG-induced drought stress tolerance of stevia (Stevia rebuadiana Bertoni). Iranian Journal of Plant Physiology. 11, (1) 3509-3522.
Angelini, L. G., A. Martini, B. Passera and S. Tavarini, 2018. Cultivation of Stevia rebaudiana Bertoni and associated challenges. Sweeteners. 35-85.
Avent, A. G., J. R. Hanson and B. H. De Oliveira, 1990. Hydrolysis of the diterpenoid glycoside, stevioside. Phytochemistry. 29, (8) 2712-2715.
Baroni-Nezhad, H., M. Karimi, H. Motaghian and F. Direkvand-Moghadam, 2021. Response of stevia (stevia rebaudiana) to copper, iron and zinc. Journal of Plant Nutrition. 1-11.
Bender, C., S. Graziano and B. F. Zimmermann, 2015. Study of Stevia rebaudiana Bertoni antioxidant activities and cellular properties. International Journal of Food Sciences and Nutrition. 66, (5) 553-558.
Bhatta, S., T. Stevanovic Janezic and C. Ratti, 2020. Freeze-Drying of Plant-Based Foods. Foods. 9, (1) 87.
Brandle, J. and P. Telmer, 2007. Steviol glycoside biosynthesis. Phytochemistry. 68, (14) 1855-1863.
Chong, K. and Y. Y. Lim, 2012. Effects of drying on the antioxidant properties of herbal tea from selected vitex species. Journal of Food Quality. 35, (1) 51-59.
Clemente, C., L. G. Angelini, R. Ascrizzi and S. Tavarini, 2021. Stevia rebaudiana (Bertoni) as a multifunctional and sustainable crop for the mediterranean climate. Agriculture. 11, (2) 123.
Criado, M. N., F. J. Barba, A. Frígola and D. Rodrigo, 2014. Effect of Stevia rebaudiana on oxidative enzyme activity and its correlation with antioxidant capacity and bioactive compounds. Food and bioprocess technology. 7, (5) 1518-1525.
Gasmalla, M. a. A., R. Yang, I. Amadou and X. Hua, 2014. Nutritional composition of Stevia rebaudiana Bertoni leaf: effect of drying method. Tropical Journal of Pharmaceutical Research. 13, (1) 61-65.
Haard, N. F. 1984. Postharvest physiology and biochemistry of fruits and vegetables. ACS Publications
Haas, G., H. Prescott Jr and C. Cante, 1974. On rehydration and respiration of dry and partially dried vegetables. Journal of Food Science. 39, (4) 681-684.
Hajihashemi, S. and J. M. Geuns, 2013. Free radical scavenging activity of steviol glycosides, steviol glucuronide, hydroxytyrosol, metformin, aspirin and leaf extract of Stevia rebaudiana. Free Radicals and Antioxidants. 3, S34-S41.
Halim, A. A., Z. M. Zain, A. Mubarak and F. T. Ahmad, 2019. Effect of different drying methods on antioxidant properties, stevioside and rebaudioside A contents of stevia (Stevia rebaudiana bertoni) leaves. Asian J Agric & Biol. 7, (1) 61-68.
Hsu, F.-L., C.-C. Hou, L.-M. Yang, J.-T. Cheng, T.-C. Chi, P.-C. Liu and S.-J. Lin, 2002. Microbial transformations of isosteviol. Journal of Natural Products. 65, (3) 273-277.
Javed, R., B. Yücesan and E. Gurel, 2018. Hydrogen peroxide-induced steviol glycosides accumulation and enhancement of antioxidant activities in leaf tissues of Stevia rebaudiana Bertoni. Sugar tech. 20, (1) 100-104.
Karimi, M., A. Ahmadi, J. Hashemi, A. Abbasi, S. Tavarini, A. Pompeiano, L. Guglielminetti and L. G. Angelini, 2016. The positive role of steviol glycosides in stevia (Stevia rebaudiana Bertoni) under drought stress condition. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology. 150, (6) 1323-1331.
Karimi, M., J. Hashemi, A. Ahmadi, A. Abbasi and M. Esfahani, 2014. Study on the bioactivity of steviol and isosteviol in stevia (Stevia rebaudiana Bertoni). Acta physiologiae plantarum. 36, (12) 3243-3248.
Kennelly, E. J. 2001. Sweet and non-sweet constituents of Stevia rebaudiana. In Stevia:81-99: CRC Press. Number of 81-99 pp.
Kovačević, D. B., F. J. Barba, D. Granato, C. M. Galanakis, Z. Herceg, V. Dragović-Uzelac and P. Putnik, 2018. Pressurized hot water extraction (PHWE) for the green recovery of bioactive compounds and steviol glycosides from Stevia rebaudiana Bertoni leaves. Food Chemistry. 254, 150-157.
Kroyer, G., 2010. Stevioside and Stevia-sweetener in food: application, stability and interaction with food ingredients. Journal für Verbraucherschutz und Lebensmittelsicherheit. 5, (2) 225-229.
Lemus-Mondaca, R., K. Ah-Hen, A. Vega-Gálvez, C. Honores and N. O. Moraga, 2016. Stevia rebaudiana leaves: effect of drying process temperature on bioactive components, antioxidant capacity and natural sweeteners. Plant Foods for Human Nutrition. 71, (1) 49-56.
Lewicki, P. P., 1998. Effect of pre‐drying treatment, drying and rehydration on plant tissue properties: A review. International Journal of Food Properties. 1, (1) 1-22.
Momtazi-Borojeni, A., S.-A. Esmaeili, E. Abdollahi and A. Sahebkar, 2017. A review on the pharmacology and toxicology of steviol glycosides extracted from Stevia rebaudiana. Current Pharmaceutical Design. 23, (11) 1616-1622.
Pandey, M. and S. Chikara, 2015. Effect of salinity and drought stress on growth parameters, glycoside content and expression level of vital genes in steviol glycosides biosynthesis pathway of Stevia rebaudiana (Bertoni). International Journal of Genetics, ISSN. 0975-2862.
Periche, A., M. L. Castelló, A. Heredia and I. Escriche, 2015. Influence of drying method on steviol glycosides and antioxidants in Stevia rebaudiana leaves. Food Chemistry. 172, 1-6.
Shock, C., 1982. Rebaudi's stevia: natural noncaloric sweeteners. California Agriculture. 36, (9) 4-5.
Singh, S. and G. Rao, 2005. Stevia: The herbal sugar of 21 st century. Sugar tech. 7, (1) 17-24.
Srivastava, S. and M. Srivastava, 2014. Morphological changes and antioxidant activity of Stevia rebaudiana under water stress. American Journal of Plant Sciences. 5, (22) 3417.
Xu, D., Y. Li, J. Wang, A. K. Davey, S. Zhang and A. M. Evans, 2007. The cardioprotective effect of isosteviol on rats with heart ischemia-reperfusion injury. Life Sciences. 80, (4) 269-274.
Zamani, A., M. Karimi, A. Abbasi-Surki and F. Direkvand-Moghadam, 2021. The effect of humic acid application on Stevia (Stevia rebaudiana) growth and metabolites under drought stress. Iranian Journal of Plant Physiology. 11, (3) 3651-3658.