Methyl jasmonate and salicylic acid effect on the production of metabolites in Portulaca oleracea L. suspension culture

Subject Areas : Phytochemistry

Fereshte Enaiate ¹ , Monireh Ranjbar ² , Sarvenaz Bigham Soostani ³

1 - Department of biology, Falavarjan Branch, Islamic Azad University
2 - Department of Biology, Falavarjan Branch, Islamic Azad University
3 - Department of Biology, Faculty of Marian Science and Technology, , Hormozgan University

Received: 2023-10-25 Accepted : 2024-03-04 Published : 2024-08-05

Keywords: Antioxidant capacity, Alkaloids, JA, Purslane, SA,

Abstract :

Purslane is a medicinal herb that is used as an antioxidant mostly because of its secondary metabolites. One of the most important biotechnological methods for changing the production pattern of secondary metabolites is the elicitation of cell suspensions. In the present study, the effects of JA (Jasmonic Acid ) and SA (Salicylic acid ) growth hormones were investigated on bioactive compounds of Portulaca oleracea L. suspension culture. Stem segments were cultured in MS medium with BAP and 2,4-D for callus induction. Cell suspensions were initiated from the callus and after elicitation with JA and SA, metabolites were extracted. Antioxidant capacity, total phenol, flavonoids, saponins, alkaloids and terpenoids were measured in the treated groups. Concomitant use of JA and SA increased antioxidant capacity compared to individual treatments. The increase in total phenol and flavonoids was almost proportional to the increase in antioxidant capacity. The amount of saponin in combined JA treatments (250 μM) with both concentrations of SA was the same as in single SA treatments. JA and SA combination treatment at both concentrations had the same effect on alkaloid production. The highest amount of terpenoids was induced with SA treatment with a concentration of 50 μM. Simultaneous treatment of P. oleracea L. suspension with JA and SA, increased antioxidant capacity by increasing the bioactive compounds of total phenol, alkaloids and terpenoids

References:

Ali, M.B., E.J. Hahn and K.Y. Paek. 2007. Methyl jasmonate and salicylic acid induced oxidative stress and accumulation of phenolics in Panax ginseng bioreactor root suspension cultures. Molecules 12, 607–621.
Ali, M. and H. Abbasi. 2014. Light-induced fluctuations in biomass accumulation, secondary metabolites production and antioxidant activity in cell suspension cultures of Artemisia absinthium L. J. Photochem. Photobiol. B: Biology 140, 223- 227.
Ali, M., B.H. Abbasi and G.S. Ali. 2015. Elicitation of antioxidant secondary metabolites with jasmonates and gibberellic acid in cell suspension cultures of Artemisia absinthium L. Plant Cell Tiss Org Cult. (PCTOC). 120,1099-106.
AÇikgÖz, M.A., S.M. Kara, A. Aydün, M.M. Özcan and E. Batiay. 2019. Effects of methyl jasmonate and salicylic acid on the production of camphor and phenolic compounds in cell suspension culture of endemic Turkish yarrow (Achillea gypsicola) species. Turk. J. Agric. For. 43,351-359.
Ajanal, M., M. Gundkalle and Sh. Nayak. 2012. Estimation of total alkaloid in Chitrakadivati by UV-spectrophotometer. Anc. Sci. Life. 31, (4) 198-201.
Andrys, D., D. Kulpa, M. Grzeszczuk, B. Białecka. 2018. Influence of jasmonic acid on the growth and antimicrobial and antioxidant activities of Lavandula angustifolia Mill. Propagated in vitro. Folia. Hortic. 30, 3.
Arias, M., M. Angarita, J.M. Restrepo and L.M. Caicedo. 2010. Elicitation with methyl-jasmonate stimulates peruvoside production in cell suspension culture of Thevetia peruviana. In vitro Cell Dev. Biol. Plant 46,233-238.
Caarls, L., C.M.J. Pieterse and S.C.M. Van Wees. 2015. How salicylic acid takes transcriptional control over jasmonic acid signaling. Front Plant Sci. 6,170.
Chaves, N., A. Santiago and J.C. Alias. 2020. Quantification of the antioxidant activity of plant extracts: analysis of sensitivity and hierarchization based on the method used. Antioxidants (Basel). 9,(1) 76.
Cheruvathur, M.K. and T.D. Thomas. 2014. Effect of plant growth regulators and elicitors on rhinacanthin accumulation in hairy root cultures of Rhinacanthus nasutus (L.) Kurz. Plant Cell Tiss. Org. Cult. 118,169-177.
Chung, I-M., K. Rekha and G. Rajakumar. 2017. Thiruvengadam M. Jasmonic and salicylic acids enhanced phytochemical production and biological in cell suspension culture of spine gourd (Momordica dioiicaroxb). Acta Biol. Hung. 68(1),88-100.
De Geyter, N., A. Gholami, S. Goormachtig and A. Goossens. 2012. Transcriptional machineries in jasmonate-elicited plant secondary metabolism. Trends Plant Sci. 17(6), 349-59.
Dogan, M. 2022. Influence of different concentrations of murashige and skoog medium on multiple shoot regeneration of Staurogyne repens (nees) kuntze. Eng. Technol. Appl. Sci. Res. 7, 61-67.
Fernández-Poyatos, M.D.P., E. Llorent-Martínez and A. Ruiz-Medina. 2021. Phytochemical composition and antioxidant activity of Portulaca oleracea: Influence of the steaming cooking process. Foods 10, 94.
Gadzovska, S., S. Maury, A. Delaunay, M. Spasenoski, C. Joseph and D. Hagège. 2007. Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones. Plant Cell Tiss. Org. Cult. 89, 1-13.
Gadzovska, S., S. Maury, A. Delaunay, M. Spasenoski, D. Hagège, D. Courtois and C. Joseph. 2013. The influence of salicylic acid elicitation of shoots, callus, and cell suspension cultures on production of naphtodianthrones and phenylpropanoids in Hypericum perforatu L. Plant Cell Tiss. Org. Cult. 113,25–39.
Ghanati, F., S. Bakhtiarian, B.M. Parast and M.K. Behrooz. 2014. Production of new active phytocompounds by Achillea millefolium L. after elicitation with silver nanoparticles and methyl jasmonate. Biosci. Biotechnol. Res. Asia. 11,391-399. https://doi.org/10.13005/bbra/1287
Halder, M., S. Sayantik, S. Jha. 2019. Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures. Eng. Life Sci. 19(12), 880-895.
Ho, T-T., H.N. Murthy and S-Y. Park. 2020. Methyl jasmonate induced oxidative stress and accumulation of secondary metabolites in plant cell and organ cultures. Int. J Mol. Sci. 21(3),716.
Khan, T., T. Khan, C. Hano and B.H. Abbasi. 2019 Effects of chitosan and salicylic acid on the production of pharmacologically attractive secondary metabolites in callus cultures of Fagonia indica. Ind. Crops Prod. 129,525-535.
Le, A.V., S.E. Parks, M. Nguyen and P.D. Roach. 2018. Improving the vanillin-sulphuric acid method for quantifying total saponins. Technologies, 6, 84.
Li, Q., J. Zheng, S. Li, G. Huang, S.J. Skilling and L. Wang. 2017. Transporter-mediated nuclear entry of jasmonoyl-isoleucine is essential for jasmonate signaling. Mol. Plant 10,695–708.
Lu, M.B., H.L. Wong and W.L. Teng. 2001. Effects of elicitation on the production of saponin in cell culture of Panax ginseng. Plant Cell. Rep. 20,674–677.
Matsuura, H.N., S. Malik, F. Costa, M. Yousefzadi, M.H. Mirjalili and R. Arroo. 2017. Specialized plant metabolism characteristics and impact on target molecule biotechnological production. Mol. Biotechnol. 60, 169–183.
Miclea, I., A., Suhani, M. Zahan and A. Bunea. 2020. Effect of jasmonic acid and salicylic acid on growth and biochemical composition of In-vitro propagated Lavandula angustifolia Mill. Agronomy 10,1722.
Moghadam, Y.A., K. Piri, B. Bahramnejad and T. Ghiasvand. 2014. Dopamine production in hairy root cultures of Portulaca oleracea (Purslane) using Agrobacterium rhizogenes. J. Agr. Sci. Tech. 16,409-420.
Naik, P.M. and J.M., Al-Khayri. 2016. Abiotic and biotic elicitor’s role in secondary metabolites production through in vitro culture of medicinal plants. In abiotic and biotic stress in plants recent advances and future perspectives; Shanker, A.K., Shankar, C., Eds.; InTech: Rijeka, Croatia, pp. 247–277
Ncube, B., J. Finnie and J. Van Staden. 2012. Quality from the field: the impact of environmental factors as quality determinants in medicinal plants. S. Afr. J Bot. 82, 11-20.
Park, C.H., H.J. Yeo, Y.E. Park, S.W. Chun, Y.S. Ching and S.Y. Lee. 2019. Influence of chitosan, salicylic acid and jasmonic acid on phenylpropanoid accumulation in ggerminated buckwheat (Fagopyrum esculentum Moench). Foods. 8(5), 153.
Pękal, A. and K., Pyrzynska. 2014. Evaluation of aluminum complexation reaction for flavonoid content assay. Food Anal. Methods. 7,1776-1782.
Rbelo, M.C., W.Y. Bang, V .Nair, R.E. Alves and D.A. Jacobo-Velazquez. 2020. UVC light modulates vitamin C and phenolic biosynthesis in acerola fruit: role of increased mitochondria activity and ROS production. Sci. Rep. 10,21972
Rincón-Pérez, J., L. Rodríguez-Hernández, V.M. Ruíz-Valdiviezo, M. Abud-Archila, M.C. Luján-Hidalgo and N. Ruiz-Lau. 2016. Fatty acids profile, phenolic compounds and antioxidant capacity in elicited callus of Thevetia peruviana (Pers.) K. Schum. J. Oleo. Sci. 65,311-318.
Rodríguez-Sáncheza, L.K., J.E. Pérez-Bernala, M.A. Santamaría-Torresc, X. Marquínez-Casasb, L.E. Cuca-Suáreza and J.A. Prieto-Rodríguezc. 2020. Effect of methyl jasmonate and salicylic acid on the production of metabolites in cell suspensions cultures of Piper cumanense (Piperaceae). Biotechnol. Rep. 28,e00559.
Saeed, S., H. Ali, T. Khan, W. Kayani and M.A. Khan. 2017. Impacts of methyl jasmonate and phenyl acetic acid on biomass accumulation and antioxidant potential in adventitious roots of Ajuga bracteosa Wall ex Benth., a high valued endangered medicinal plant. Physiol. Mol. Biol. Plants. 23(1), 229-237.
Samrot, A.V. and T., Sean. 2022. Investigating the antioxidant and antimicrobial Activity of Artocarpus heterophyllus Lam. (Jackfruit) Late. Biointerface Res. Appl. Chem. 12(3), 3019- 3033.
Scarpa, G.M., V. Prota, N. Schianchi and F. Manunta. 2022. In vitro cultures for the production of secondary metabolites. Secondary Metabolites. Eds., Ramasamy Vijayakumar, Suresh Selvapuram and Sudalaimuthu Raja.
Sedaghati, B., R. Haddad and M. Bandehpour. 2018. Efficient plant regeneration and agrobacterium-mediated transformation via somatic embryogenesis in purslane (Portulaca oleracea L.): an important medicinal plant. Plant Cell Tiss. Org. Cult. (PCTOC). 13, 1-15.
Shabani, L., A.A. Ehsanpour, G.H. Asghari and J. Emami. 2009. Glycyrrhizin production by in vitro cultured Glycyrrhiza glabra elicited by methyl jasmonate and salicylic acid. Russ. J. Plant Physiol. 56(5), 621-626.
Suzuki, H., M.S. Reddy, M. Naoumkina, N. Aziz, G.D. May and D.V. Huhman. 2005. Methyl jasmonate and yeast elicitor induce differential transcriptional and metabolic re-programming in cell suspension cultures of the model legume Medicago truncatula. Planta 220,696-707.
Taj, F., M. Ali Khan, H. Ali and R. Sher Khan. 2019. Improved production of industrially important essential oils through elicitation in the adventitious roots of Artemisia amygdalina. Plants (Basel). 8(10),430
Tukowski, A., R. Jagietto, P. Robakowski and D. Adamczyk. 2022. Adaptation of a simple method to determine the total terpenoid content in needles of coniferous trees. Plant Sci. 314,111090.
Twaij, B.M. and N. Hasan. 2022. Bioactive secondary metabolites from plant sources: Types, synthesis, and their therapeutic uses. Int J Plant Biol. 13(1), 4-14.
Wang, J., J.L. Li, J. Li, J.X. Li, S. Liu and L. Huang. 2017. Production of active compounds in medicinal plants: from plant tissue culture to biosynthesis. Chin. Herb Med. 9,115-125.
Wang, F., G. Yu and P. Liu. 2019. Transporter-Mediated subcellular distribution in the metabolism and signaling of jasmonates. Front Plant Sci. 2, 1-8.
Xu, Y., Z. Wei, C. Xue and Q. Huang. 2022. Assembly of zein–polyphenol conjugates via carbodiimide method: Evaluation of physicochemical and functional properties. Food Sci. Technol. 154,112708.
Zhao, J., C.D. Lawrence and R. Verpoorte. 2005. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol. Adv. 23(4), 283-333.
Zhang, X. and C-J., Liu. 2015. Multifaceted regulations of gateway enzyme phenylalanine ammonialyase in the biosynthesis of phenylpropanoids. Mol. Plant. 8(1), 17- 27.