Identification of QTLs related to rice seedling traits under K deficiency stress in Iranian inbred lines population

Subject Areas : Genetics

Hossein Sabouri ¹ , Abdollatif Gholizadeh ² , Sharifeh Alegh ³ , , Somayyeh Sanchouli ⁴ , Mahnaz Katouzi ⁵

1 - Department of Plant Production, Collage of Agriculture Science and Natural Resources, Gonbad Kavous University, Gonbad, Golestan, Iran
2 - Department of Plant production, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, Gonbad, Iran
3 - Department of Plant Production, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, Gonbad, Iran
4 - Department of Plant production, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, Gonbad
5 - Department of plant breeding and genetic resource, Agroscope, Route de Duillier 50, Case Postale 1012, 1260 Nyon 1, Switzerland

Received: 2022-01-02 Accepted : 2022-02-06 Published : 2022-07-01

Keywords: Marker assisted breeding, Potassium deficiency, Gene mapping, Seedling, Major QTL,

Abstract :

A mapping population of 96 inbred line derived by cross Neda (NAD) and Ahlamitaroum (ATM), was used to detect quantitative trait loci (QTLs) for fresh biomass (FB), root length (RL), shoot length (SL), root number (RN), leaf width (LW), root fresh weight (RFW), root dry weight (RDW) and K content (KC) under K deficiency condition in rice. Two parents and 96 inbred lines were phenotyped for the traits by growing them in K deficiency nutrient solution. In K deficiency, 16 QTL was able to explain a great deal of phenotypic variation in features. qSL-7a, qRL-6, qRL-10b, qRL-12b, qRN-6b, qRN-12b, qLL-6, qLL-7, qLW-6b, qLW-10b, qLW-12a, qLW-12b, qLW-12c, qKC-6b, qKC-10b and qKC-12c were on chromosomes 6, 7, 10 and 12. Their LOD are 4.732, 5.826, 5.01, 5.067, 11.346, 5.867, 5.973, 5.85, 7.077, 8.055, 5.577, 6.439, 8.012, 14.057, 6.432 and 858/6, respectively. In normal conditions, 5 QTL with a large effect were identified. qKCN-6c, qKCN-7a, qKCN-10, qKCN-12b and qKUN-6 were located on chromosomes 6, 7, 10, 12 and 6, respectively. Owing to the high percentage of explanation, the major QTLs can be a suitable candidate for marker assistance programs in recombinant Iranian rice lines after validation.

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