Evaluation of the effect of levetiracetam drug on BSA structure
Subject Areas :
1 - Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran
Keywords: BSA, structural changes, Levetiracetam, Drug-protein interaction, Spectroscopic methods,
Abstract :
Levetiracetam (LV, [S]-alpha-ethyl-2-oxo-1-pyrrolidine acetamide) is a new antiepileptic that has been used as adjunctive therapy to treat patients with intractable epilepsy. Its interaction with bovine serum albumin (BSA) is of great use for the understanding of the pharmacokinetic and pharmacodynamic mechanisms of the drug. The effect of levetiracetam drug on native BSA structure has been studied by UV-visible and circular dichroism methods. In addition, fluorescence spectroscopy was used to investigate the effect of LV on F and B conformers of BSA. UV-visible results indicated that the interaction of LV with BSA may cause the conformational changes in BSA and change the polarity of the microenvironment around tyrosine and tryptophan residues of BSA. Analysis of CD spectrum displayed an increase in content of α-helical structure of BSA induced by LV and stabilized it. This study makes an important contribution to enhance our basic knowledge of the molecular basis of drug-protein interaction, which could be applicable for the development of more efficacious antiepileptic medicines.