Optimization of Culture Conditions for Keratinase Production in Streptomyces Sp. JRS19 for Chick Feather Wastes Degradation

Subject Areas : Agricultural Extension

T.  Jayalakshmi ¹ (Dept of Bioinformatics, Bharath University, Chennai, Tamilnadu - 600073, India.)
P.  Krishnamoorthy ² (Dept of Bioinformatics, Bharath University, Chennai, Tamilnadu - 600073, India.)
G.  Ramesh kumar ³ (Dept of Bioinformatics, MIT Campus, Anna University, Chennai, Tamilnadu-600044, India)
P.  Sivamani ⁴ (Microlabs, Vellore, Tamilnadu -632 521, India.)

Received: 2015-12-25 Accepted : 2015-12-25 Published : 2011-09-01

Keywords: Enzyme, Streptomyces sp, feather, Keratinase,

Abstract :

The aim of this study was to find the optimal conditions of a newly isolated Streptomyces strain JRS 19. Screening for keratinase producing bacteria and their keratinase production were investigated. The keratinase produced by the isolate was purified by chromatographic techniques and the optimum pH maintained was 7.0 and maintained at different temperatures Viz., 20ºC, 25ºC, 30ºC, 35ºC and 40ºC. Besides temperature and pH ,the enzyme is active upon different sources and its effect on carbon, nitrogen , aminoacids were determined and recorded the protein content and enzyme activity. The carbon sources revealed that sucrose was found highly significant, whereas peptone was found highly significant in nitrogen source. The effect of keratinase on amino acid shows that valine was found highly significant. Based on this study, the physio-chemical properties of keratinase were analyzed and the results were interpreted. [T. Jayalakshmi et al. Optimization of Culture Conditions for Keratinase Production in Streptomyces Sp. JRS19 for Chick Feather Wastes Degradation. International Journal of Agricultural Science, Research and Technology, 2011; 1(3):121-125 ].

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