Morphological and Molecular Identification of Botrytis Cinerea Causal Agent of Gray Mold in Rose Greenhouses in Centeral Regions of Iran

Subject Areas : International Journal of Agricultural Science and Research

P. Khazaeli ¹ (nadarad)
H. Zamanizadeh ² (nadarad)
B. Morid ³ (nadarad)
H. Bayat ⁴ (nadarad)

Received: 2014-12-02 Accepted : 2014-12-02 Published : 2012-05-14

Keywords: Botrytis cinerea, Gray mold, Morphological and Molecular identification,

Abstract :

Botrytis cinerea is an important pathogen that causes diseases in ornamental crops. In presentresearch several greenhouses of roses located in central region of Iran were surveyed toidentify the Botrytis cinerea. A total of 80 isolates were collected from rose greenhouses incentral region of Iran. Morphological identification was based on characters such asconidiophore and conidial length. According to the results conidiophores length was [phone] (3896) μm, conidial dimension were in the range of (4)813(16)×(2)47(10) μm.Based on these characteristics, all isolates belonged to morphospecies Botrytis cinerea.Using specific primer pairs C729 and PCR molecular method, a DNA fragment of 700bpwas amplified to the gene isolates for 30 representative isolates identified by morphologicalassessment indicating the presence of Botrytis cinerea rose samples.