Moringa and Sesbania Leaves in Layer Chicken Pens as an Edible Environmental Enrichment: Effects on Performance and, Physical and Organoleptic Properties of Eggs

Subject Areas :

S. Vijayakumar ¹ , S.T.D. De Silva ² , N.S.B.M. Atapattu ³ , D. Senaratne ⁴ , M. Pagthinathan ⁵

1 - Department of Animal Science, Faculty of Agriculture, University of Ruhuna, Sri Lanka
2 - Department of Animal Science, Faculty of Agriculture, Eastern University, Sri Lanka
3 - Department of Animal Science, Faculty of Agriculture, University of Ruhuna, Sri Lanka
4 - Department of Animal Science, Faculty of Agriculture, University of Ruhuna, Sri Lanka
5 - Department of Animal Science, Faculty of Agriculture, Eastern University, Sri Lanka

Received: 2023-12-11 Accepted : 2024-07-08 Published : 2024-09-24

Keywords: cage, egg physical, enrichment, hanging, Moringa oleifera, organoleptic, Sesbania grandiflora,

Abstract :

Enrichment of layer chicken environment with physical, sensory, or stimulatory additions reported to have numerous beneficial effects. Hanging readily available tropical leguminous leafy materials such as Moringa and Sesbania in layer chicken pens can provide an additional amount of nutrients while serving as an envi-ronmental enrichment means. This study investigated the effects of hanging Moringa and Sesbania leaves as edible cage enrichments on performance, physical and organoleptic properties of egg and litter character-istics. Giving a completely randomized design experiment, 75 weeks old RIR layers (n=80) were allocated into 16 pens. Treatments were hanging two bundles of Moringa oleifera (MOL) leaves (T1), one bundle of MOL and one bundle of Sesbania grandiflora (SGL) leaves (T2), two bundles of SGL (T3) and no leaves hanging (T4). Egg production, and feed and leafy material intake were recorded for 42 days. Eggs were analyzed for physical properties. A semi-trained panel (n=25) conducted a sensory evaluation for eggs. The frequency of visiting feeders, drinkers, and leaves bundles was recorded using scan sampling techniques. Results showed that birds more preferred to eat SGL than MOL. Hanging of MOL or SGL alone or as two separate bundles increased body weight, egg weight, percentage of egg albumen, egg length, and eggshell thickness significantly. Colour, flavor, and overall acceptability of yolk color and flavor and aroma of egg white were positively influenced (P<0.05) by the hanging of leafy materials. Birds in pens with MOL and SGL exhibited more exploratory behaviors toward leaf bundles. Hanging of leafy materials increased the litter nitrogen content (P<0.05) as well. The study concludes that hanging MOL or MOL MOL leaves in layer chicken pens has positive impacts on physical and organoleptic properties and welfare while increas-ing litter nitrogen content.

References:

Abbas T.E. (2013). The use of Moringa oleifera in poultry diets. Turkish J. Vet. Anim. Sci. 37(5), 492-496.
Abdel-Wareth A.A.A. and Lohakare J. (2021). Moringa oleifera leaves as eco-friendly feed additive in diets of hy-line brown hens during the late laying period. Animals. 11(4), 1-10.
Abou-Elezz F.M.K., Sarmiento-Franco L., Santos-Ricalde R. and Solorio-Sanchez F. (2011). Nutritional effects of dietary inclu-sion of Leucaena leucocephala and Moringa oleifera leaf meal on Rhode Island Red hens’ performance. Cuban J. Agric. Sci. 45(2), 163-170.
Abou-Elkhair R., Abdo Basha H., Slouma Hamouda Abd El Naby W., Ajarem J.S., Maodaa S.N., Allam A.A. and Naiel M.A. (2020). Effect of a diet supplemented with the Moringa oleif-era seed powder on the performance, egg quality, and gene expression in Japanese laying quail under heat-stress. Animals. 10(5), 809-817.
Bakare A.G., Zindove T.J. Iji P.A., Stamatopoulos K. and Cowi-eson A.J. (2021). A review of limitations to using cassava meal in poultry diets and the potential role of exogenous mi-crobial enzymes. Trop. Anim. Health Prod. 53(4), 426-435.
Bari M.S., Cohen-Barnhouse A.M. and Campbell D.L.M. (2020). Early rearing enrichments influenced nest use and egg quality in free-range laying hens. Animals. 14(6), 1249-1257.
Bhokre C., Gadhe K. and Joshi A. (2022). Assessment of nutritional and phytochemical properties of Sesbania grandiflora flower and leaves. J. Pharm. Innov. 11(6), 90-94.
Bhosale T.R., Chavan M.K. and Dnyandeo T. (2021). Proximate analysis of Azadirachta indica and Moringa oleifera L. leaves as feed additives for ruminants. J. Pharm. Innov. 10(12), 1884-1886.
Bidura I.G.N.G., Partama I.B.G., Utami I.A.P., Candrawati D.P.M.A., Puspani E., Suasta I.M. and Warmadewi D.A. (2020). Effect of Moringa oleifera leaf powder in diets on laying hens performance, β-carotene, cholesterol, and minerals contents in egg yolk. IOP Conf. Ser. Mater. Sci. Eng. 823(1), 1-9.
Campbell D.L.M., Hinch G.N., Downing J.A. and Lee C. (2018). Early enrichment in free-range laying hens: Effects on ranging behaviour, welfare and response to stressors. Animals. 12(3), 575-584.
Campbell D.L.M., De Haas E.N. and Lee C. (2019). A review of environmental enrichment for laying hens during rearing in relation to their behavioral and physiological development. Poult. Sci. 98(1), 9-28.
Cronin G.M. and Glatz P.C. (2020). Causes of feather pecking and subsequent welfare issues for the laying hen: a review. Anim. Prod. Sci. 61(10), 990-1005.
Cronin G.M., Hopcroft R.L., Groves P.J., Hall E.J.S., Phalen D.N. and Hemsworth P.H. (2018). Why did severe feather pecking and cannibalism outbreaks occur? An unintended case study while investigating the effects of forage and stress on pullets during rearing. Poult. Sci. 97(5), 1484-1502.
Da Silva L.M.I., Almeida Paz I.C.D.L., Chaves G.H.C., Almeida I.C.D.L., Ouros C.C.D., Souza S.R.L.D., Milbradt E.L., Cal-dara F.R., Satin A.J.G., Costa G.A.D. and Glavina A.S.G. (2021). Behaviour and animal welfare indicators of broiler chickens housed in an enriched environment. PLoS One. 16(9), e0256963.
Esonu B.O., Opara M.N., Okoli I.C., Obikaonu H.O., Udedibie C. and Iheshiulor O.O.M. (2006). Physiological response of lay-ing birds to neem (Azadirachta indica) leaf meal-based diets: Body weight organ characteristics and hematology. Online J. Health Allied Sci. 5(2), 1-7.
Garcia R.G., Gandra E.R.S., Burbarelli M.F.C., Valentim J.K., Felix G.A., Lopes B.A., Bacha F.B., Melo C.M.F., Silva J.P., Komiyama C.M. and Caldara F.R. (2021). Moringa oleifera: An alternative ingredient to improve the egg quality of Japa-nese quail. Arq. Bras. Med. Vet. Zootec. 73, 721-732.
Glover-Amengor M., Aryeetey R., Afari E. and Nyarko A. (2017). Micronutrient composition and acceptability of Moringa oleif-era leaf-fortified dishes by children in Ada-East district, Gha-na. Food Sci. Nutr. 5(2), 317-323.
Gobezie E. (2021). Supplementation of Moringa oleifera leaf meal in layer chickens’ feed: A review. World's Vet. J. 11(2), 202-207.
Gobezie E. (2022). Effect of Neem (Azadirachta indica) leaf powder on the growth performance and carcass quality of broiler chicken-A review. J. Livest. Sci. 21(13), 152-158.
Hayat Z., Cherian G., Pasha T.N., Khattak F.M. and Jabbar M.A. (2010). Sensory evaluation and consumer acceptance of eggs from hens fed flax seed and 2 different antioxidants. Poult. Sci. 89(10), 2293-2298.
Henuk Y.L. and Dingle J.G. (2002). Practical and economic ad-vantages of choice feeding systems for laying poultry. Worlds Poult. Sci. 58(2), 199-208.
Ige A.O., Odunsi A.A., Akinlade J.A. and Ojedapo L.O. (2006). Gliricidia leaf meal in layer’s diet: Effect on performance, nu-trient digestibility and economy of production. J. Anim. Vet. Adv. 5(6), 483-486.
Kejela M., Diba D., Yusuf H., Ferede B. and Duguma G. (2020). Feed intake, weight gain and profitability of sasso chickens fed locally formulated rations at Wollega university, Nekemte, Ethiopia. World Appl. Sci. J. 38(5), 404-415.
Khan M.K.A., Akbar M.A., Khaleduzzaman A.B.M. and Rahman M.E. (2009). Utilization of Leucaena and Sesbania leaf meals as protein. Bangladesh J. Anim. Sci. 38(1), 123-131.
Kumar U., Murthy H.N.N., Singh K.C., Mahadevappa D., Ra-jeshwari Y.B., Siddeshwara N.C., Mateen A. and Guruprasad R. (2017). Biomass yield and chemical composition of sesba-nia. Int. J. Sci. Environ. 6(6), 3264-3269.
Liebers C.J., Schwarzer A., Erhard M., Schmidt P. and Louton H. (2019). The influence of environmental enrichment and stock-ing density on the plumage and health conditions of laying hen pullets. Poult. Sci. 98(6), 2474-2488.
Liyanage R., Jayathilaka C., Perera O.S., Kulasooriya S.A., Jay-awardana B.C. and Wimalasiri S. (2014). Protein and micro-nutrient contents of Moringa oleifera (Murunga) leaves col-lected from different localities in Sri Lanka. Asian J. Agric. Food Sci. 2(4), 264-269.
Lu W., Wang J., Zhang H.J., Wu S.G. and Qi G.H. (2016). Evalu-ation of Moringa oleifera leaf in laying hens: Effects on laying performance, egg quality, plasma biochemistry and organ his-topathological indices. Italian J. Anim. Sci. 15(4), 658-665.
Mabusela S.P., Nkukwana T.T., Mokoma M. and Muchenje V. (2018). Layer performance, fatty acid profile and the quality of eggs from hens supplemented with Moringa oleifera whole seed meal. South African J. Anim. Sci. 48(2), 234-243.
Mahfuz S. and Piao X.S. (2019). Application of Moringa (Mor-inga oleifera) as natural feed supplement in poultry diets. Animals. 9(7), 431-437.
McAdie T.M., Keeling L.J., Blokhuis H.J. and Jones R.B. (2005). Reduction in feather pecking and improvement of feather con-dition with the presentation of a string device to chickens. Appl. Anim. Behav. Sci. 93(1), 67-80.
Melesse A., Tiruneh W. and Negesse T. (2011). Effects of feeding Moringa stenopetala leaf meal on nutrient intake and growth performance of Rhode Island Red chicks under tropical cli-mate. Trop. Subtrop. Agroecosyst. 14(2), 485-492.
Okereke C.O., Okereke I.H., Olaleru I.F. and Ukonu C.E. (2022). Potentials of sweet potato foliage as feed ingredient in Broiler diets. Nigerian Agric. J. 53(3), 171-174.
Paguia H.M., Paguia R.Q., Balba C. and Flores R.C. (2014). Utilization and Evaluation of Moringa oleifera L. as Poultry Feeds. APCBEE Proc. 8(2013), 343-347.
Putri F., Suryantini A. and Mulyo J.H. (2021). Consumer preference towards layer chicken eggs and native chicken eggs in Yogyakarta. Adv. Econ. Bus. Manag. 199, 181-189.
Schreiter R., Damme K., Klunker M., Raoult C., von Borell E. and Freick M. (2020a). Effects of edible environmental enrich-ments during the rearing and laying periods in a littered avi-ary-Part 2: Physical development of pullets and performance, egg quality, and carcass composition in laying hens. Poult. Sci. 99(12), 6685-6696.
Schreiter R., Damme K. and Freick M. (2020b). Edible environ-mental enrichments in littered housing systems: Do their ef-fects on integument condition differ between commercial lay-ing hen strains? Animals. 10(12), 2434-2441.
Sharmin F., Sarker M.S.K., Sarker N.R. and Faruque S. (2021). Dietary effect of Moringa oleifera on native laying hens’ egg quality, cholesterol and fatty-acid profile. Italian J. Anim. Sci. 20(1), 1544-1553.
Son J.H., Ragland D. and Adeola O. (2002). Quantification of digesta flow into de caeca. Br. Poult. Sci. 43, 322-324.
SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 20. Chicago SPSS Inc., USA.
Steenfeldt S., Kjaer J.B. and Engberg R.M. (2007). Effect of feed-ing silages or carrots as supplements to laying hens on produc-tion performance, nutrient digestibility, gut structure, gut mi-croflora and feather pecking behaviour. Br. Poult. Sci. 48(4), 454-468.
Sugano M. and Matsuoka R. (2021). Nutritional viewpoints on eggs and cholesterol. Foods. 10(3), 494-503.
Tamiru M., Ashagrie S., Alkhtib A., Getachew M., Demeke S., Hassen W., Worku Z. and Burton E. (2020). Performance of broilers and layers supplemented with Moringa stenopetala leaf meal under hot humid tropical conditions. Anim. Prod. Sci. 60(17), 1987-1994.
Ung P., Kang K., Neang S., Cheat S. and Mob S. (2012). Effect of using Sun-Dried sesbania leaves in concentrate on growth rate of quail. Int. J. Environ. Rural Dev. 3(2), 109-113.
van Staaveren N., Ellis J., Baes C.F. and Harlander-Matauschek A. (2021). A meta-analysis on the effect of environmental en-richment on feather pecking and feather damage in laying hens. Poult. Sci. 100(2), 397-411.
Voemesse K., Teteh A., Nideou D., N’nanlé O., Tété-Benissan A., Oke O., Gbeassor M., Decuypere E. and Tona K. (2019). Chemical composition and some functional properties of Mor-inga, Leucaena and Gliricidia leaf meals. European J. Poult. Sci. 83, 1-12.
Xu D., Shu G., Liu Y., Qin P., Zheng Y., Tian Y., Zhao X. and Du X. (2022). Farm environmental enrichments improve the wel-fare of layer chicks and pullets: A comprehensive review. An-imals. 12(19), 2610-2617.