Effect Analysis of Prolactin (PRL) Gene Polymorphisms on Chicken Egg Productivity (Gallus gallus domesticus) BC1 from Crossbreeding between Pelung and Layer Chicken

Subject Areas : Camel

R. Kilatsih ¹ , A.B.I. Perdamaian ² , T. Joko ³ , S.H. Purwanto ⁴ , B.S. Daryono ⁵

1 - Department of Tropical Biology, Genetics and Breeding Laboratory, Faculty of Biology, Universitas Gadjah Mada, Teknika Selatan, Sleman, Daerah Istimewa Yogyakarta, Indonesia
2 - Department of Tropical Biology, Genetics and Breeding Laboratory, Faculty of Biology, Universitas Gadjah Mada, Teknika Selatan, Sleman, Daerah Istimewa Yogyakarta, Indonesia
3 - Department of Tropical Biology, Animal Systematic Laboratory, Faculty of Biology, Universitas Gadjah Mada, Teknika Selatan, Sleman, Daerah Istimewa Yogyakarta, Indonesia
4 - Department of Tropical Biology, Animal Systematic Laboratory, Faculty of Biology, Universitas Gadjah Mada, Teknika Selatan, Sleman, Daerah Istimewa Yogyakarta, Indonesia
5 - Department of Tropical Biology, Genetics and Breeding Laboratory, Faculty of Biology, Universitas Gadjah Mada, Teknika Selatan, Sleman, Daerah Istimewa Yogyakarta, Indonesia

Received: 2020-01-21 Accepted : 2020-04-15 Published : 2020-12-01

Keywords: Layer, egg, Proximate analysis, pelung, promoter gene PRL,

Abstract :

Prolactin (PRL) Gene in chicken was closely related to broodiness and brooding behavior that located at chromosome 2. This research was conducted to detect insertion/deletion (in/del) 24-bp polymorphism in the prolactin promoter gene and its association with the BC1 hybrid chicken egg productivity. Procedures of this research conducted are chicken maintenance, egg collection for 23 weeks of production, eggs proximate quality (water, ash, protein, carbohydrate, fat, and fiber percentage), DNA isolation, PRL gene amplification, and visualizing DNA bands on PRL promoters. The results showed the average number of cumulative eggs of BC1 chicken for 23 weeks was 42.9 eggs, lower than layer (104.34 eggs), and higher than pelung (30.17 eggs). Analysis using the pearson correlation test shows the frequency of insertion-deletion polymorphism alleles on the PRL promoter has a signification value of 0.521 and correlation value 0,684, so it is concluded that there is no correlation (P>0.05). Proximate test results of BC1 whole raw egg chicken has characteristics of low fat (3.2%) and high carbohydrate (9.1%) compared to commercial egg and has a calorie content of 98.37/100 g of the sample.

References:

Badan Pusat Statistik. (2017). Jumlah Penduduk Indonesia melalui tingkat Provinsi. Available at: http://www.bps.go.id/.

Cui J.X., Du H.L., Liang Y., Deng X.M., Li N. and Zhang X.Q. (2006). Association polymorphism in the promoter region of chicken prolactin with egg production. Poult. Sci. 85, 26-31.

Direktorat Jenderal Peternakan. (2017). Statistika Peternakan. Available at: http://ditjennak.deptan.go.id/.  

Emamgholi-Begli H., Zerehdaran S., Hassani S., Abbasi M.A. and Khan-Ahmadi A. (2010). Polymorphism in prolactin and PEPCK-Cgenes and its association with economic traits in native fowl of Yazd province. Iranian J Biotechnol. 8, 172-177.

Ernanto A. (2017). Asosiasi Polimorfisme gen PRL dan IGF-1 terhadap produktivitas telur ayam(Gallus gallus domesticus) F₁ hasil persilangan Ayam Pelung dan Layer. Ph D. Tesis. Gadjah Mada Univ., Yogyakarta, Indonesia.

Freeman M.E., Kanyicska B., Lerant A. and Nagy G. (2000). Prolactin: Structure, function, and regulation of secretion. Physiol. Rev. 80, 1523-1631.

Haryono H. (2000). Langkah-Langkah Teknis Uji Kualitas Telur Konsumsi Ayam Ras. Temu Teknis Fungsional Non Peneliti. Balai Penelitian Ternak, Bogor.

Jiang R.S., Xu G.Y., Zhang X.Q. and Yang N. (2005). Association of polymorphisms for prolactin and prolactin receptor genes with broody traits in chickens. Poult. Sci. 84, 839-845.

Kondrashov A.S. and Bogozin I.B. (2004). Context of deletions and insertions in human coding sequence. Hum. Mutat. 23, 177-185.

Lewin B. (1997). Genes VI. Oxford University Press, Inc., New York.

Lin X.J., Zhang R., Jiang S., Elmashad H.M. and Mitloehner F. (2016). Nutrient flow and distribution in conventional cage, enriched colony, and aviary layer houses. Poult. Sci. 95, 213-224

Liu L., Zhao X., Li D. and Liu Y. (2011). Polymorphism of prolactin receptor gene and its association with egg production traits in erlang mountainous chicken. Asian J. Anim. Vet. Adv. 7(11), 1183-1190.

Miao Y.W., Burt D.W., Paton I.R., Sharp P.J. and Dunn I.C. (1999). Mapping of the prolactin gene to chicken chromosome 2. Anim. Genet. 30, 473-480.

Montaldo H.H. and Meza-Herrera C.A. (1998). Use of molecular markers and major genes in the genetic improvement of livestock. Elect. J. Biotechnol. 1(2), 59-74.

Nataamijaya A.G., Sitorus P., Bintang I.A.K., Haryono H., and Bunyamin E. (1993). Pertumbuhan badan ayam silangan (pelung×pelung) yang di pelihara di pedesaan. Pp. 232-235 in Proc. Natl. Semin. Domest. Chicken Livest. Dev. Through Welcom. Cooperat., Bandung, Indonesia.

Rashidi H., Rahimi-Mianji G., Farhadi A. and Gholizadeh M. (2012). Association of prolactin and prolactin receptor gene polymorphisms with economic traits in breeder hens of indigenous chickens of Mazandaran province. Iranian J. Biotechnol. 10, 129-135.

Romanoff A.L. and Romanoff A.J. (1997). The Avian Eggs. John Willey and Sons, New York.

Saragih H.T.S.S.G. and Daryono B.S. (2012). Effect of high-protein diet on body weight and pectoralis thoracicus muscle performance on pelung and broiler chicken (Gallus gallus domesticus). Anim. Prod. J. 14(3), 199-204.

Sarica M. and Erensayin C. (2014). Poultry Products. Pp. 89-138 in Poultry Science. M. Turkoglu and M. Sarica, Eds., Bey Ofset, Ankara, Turkey.

Singh U.A., Kumari M. and Iyengar S. (2018). Method for improving the quality of genomic DNA obtained from minute quantities of tissue and blood samples using Chelex 100 resin. Biol. Proc. Online. 20, 12-21.

SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 20. Chicago SPSS Inc., USA.

Suceveanu A.I., Mazilu L. and Suceveanu A.P. (2014). COL11A1-genetic biomarker targeted in stool samples for early diagnosis of colorectal cancer in patients at risk. Pp. 406-407in Colorectal Cancer-Surgery, Diagnostics and Treatment. J.S. Khan, Ed. InTech Publisher, United States.

Upchuch D.A., Shankarappa R. and Mullins I. (2000). Position and degree of mismatches and the mobility DNA heteroduplexes. Nucleic Acids Res. 28(12), 1-7.

Utama I.V., Perdamaian A.B.I. and Daryono B.S. (2018). Plumage uniformity, growth rate and growth hormone polymorphism in Indonesian hybrid chickens. Int. J. Poult. Sci. 17, 486-492.

Wilkanowska A., Mazurowski A., Mroczkowski S. and Kokoszyñski D. (2014). Prolactin (PRL) and prolactin receptor (PRLR) genes and their role in poultry production traits. Folia Biologica. 62(1), 1-8.