Association between Insulin–Like Growth Factor I Polymorphism and Early Growth Traits in Iranian Zandi Sheep, Found Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP)
Subject Areas : Camel
ف. نظری
1
(
Department of Animal Science, Faculty of Agriculture, Karaj Branch, Islamic Azad University, Karaj, Iran
)
ع. نوشری
2
(
Department of Animal Science, Faculty of Agriculture, Karaj Branch, Islamic Azad University, Karaj, Iran
)
ب. همتی
3
(
Department of Animal Science, Faculty of Agriculture, Karaj Branch, Islamic Azad University, Karaj, Iran
)
Keywords: Zandi sheep, early growth traits, allele frequency, genotype frequency, <i>IGF1</i>,
Abstract :
Early growth traits in sheep are economically important. Breeding for these traits has been shown to be problematic using quantitative genetic methods, particularly in native sheep herds. Molecular genetics is useful for sheep breeding. The gene for insulin-like growth factor 1 (IGF1) is one of the important candidate genes known for ovine early growth traits. The aim of this study was to investigate IGF1 polymorphism in exon 1 and its association with some early growth traits in Iranian Zandi sheep. For this purpose, 120 male and female lambs were selected randomly at up to 5 months old and blood samples were taken individually. Also, phenotypic records were collected from related farms. Genomic DNA was extracted by a salting-out method. A 265 bp region in exon 1 of IGF1 was amplified by polymerase chain reaction (PCR), then genotyped using the restriction endonuclease HaeII. Two alleles, here called A and B, were found. Examination using PopGene32 showed allele frequencies of 0.47 A and 0.53 B. Genotype % frequencies were 28.33 AA, 37.50 AB and 34.17 BB. Chi-squared and G-squared tests showed that the population was not at Hardy-Weinberg equilibrium (P<0.01). An association study in SAS 9.2 found no significant effect of IGF1 genotype on any of these early growth traits: birth weight, average daily gain up to age five months and weight at age five months. However the AA genotype was linked to the highest values of birth weight. IGF1 polymorphism should be investigated as a molecular marker along with other polygene effects for growth traits in native sheep breeding programs.
Adam C.L., Gadd T.S., Findlay P.A. and WathesD.C. (2000). IGF-I stimulation of luteinizing hormone secretion, IGF-binding proteins (IGFBPs) and expression of mRNAs for IGFs, IGF receptors and IGFBPs in the ovine pituitary gland. J. Endocrinol. 166, 247-254.
Chelongar R., Hajihosseinlo A. and Ajdary M. (2014). The Effect of Igf-1 and Pit-1 genes polymorphisms on fat-tail measurements (fat-tail dimensions in Makooei sheep). American-Eurasian Network. Sci. Inform. J. 4(8), 862-867.
Cianfarani S., Clemmons D.R. and Savage M.O. (2005). IGF1 and IGF Binding Proteins. Karger Publishing, Switzerland.
Ge W., DavisM.E. and Hines H.C. (1997). Two SSCP alleles detected in the 5'-flanking region of bovine IGFI gene. Anim. Genet. 28, 155-156.
Ge W., DavisM.E., Hines H.C., Irvin K.M. and Simmen R.C. (2001). Association of a genetic marker with blood serum insulin-like growth factor-I concentration and growth traits in Angus cattle. J. Anim. Sci. 79, 1757-1762.
Ghafouri-Kesbi F., Eskandarinasab M. and Hassanabadi A. (2008). Investigation of genetic variability and inbreeding characteristics in a population of Zandi sheep. Canadian J. Anim. Sci. 88, 409-417.
Gholibeikifard A., Aminafshar M. and Hosseinpour Mashhadi M. (2013). Polymorphism of IGFI and ADRB3 genes and their association with growth traits in the Iranian Baluchi sheep. J. Agric. Sci. Technol. 15, 1153-1162.
Grossi Ddo A., Buzanskas M.E., Grupioni N.V., de Paz C.C., Regitano L.C., de Alencar M.M., Schenkel F.S. and Munari D.P. (2015). Effect of IGF1, GH and PIT1 markers on the genetic parameters of growth and reproduction traits in Canchim cattle. Mol. Biol. Rep. 42(1), 245-251.
He J.N., Zhang B.Y., Chu M.X., Wang P.Q., Feng T., Cao G.L., Di R., Fang L., Huang D.W., Tang Q.Q. and Li N. (2012). Polymorphism of insulin-like growth factor 1 gene and its association with litter size in small Tail Han sheep.Mol Biol. Rep. 39(10), 9801-9809.
Honarvar M., Moradi-Shahrebabak M., Sadeghi H., Behzadi S.H. and Mohamadabadi M.R. (2012). Study of the polymorphism at the 5’flanking region of the ovine IGFI Gene and its association with carcass traits in Zel (tailed) and Lori-Bakhtiari (fat-tailed) breed sheep using PCR-SSCP. J. Agric. Biotechnol. 4(2), 71-82.
Khojasteh K.M., Emam Jome N.K. and Torshizy R.V. (2006). A study of pelt and growth traits in Zandi sheep. Proc. New Zealand Soc. Anim. Prod. 66, 206-208.
Mehmannavaz Y., Amirinia C., Bonyadi M. and Vaez Torshizi R. (2010). Association of IGF1 gene polymorphism with milk production traits and paternal genetic trends in Iranian Holstein bulls. African J. Microbiol. Res. 4, 110-114.
Munoz A., Trigo P., Riber C., Malonda V. and Castejon F. (2011). A study of serum insulin-like growth factor type 1 (IGF1) concentrations in resting untrained Andalusian horses: influence of age and gender. Vet. Med. 56(5), 31-242.
Purbayramian F., Hashemi A., Mardani K., Ghaderzadeh M., Azizi P., Farhadian M. and Biabani P. (2013). Association between polymorphism IGFI gene exon 1 and growth traits in Moghani sheep. Anim. Sci. J. (Pajouhesh and Sazandegi). 106, 265-272.
Ranjbari M., Hashemi A., Mardani K. and Darvishzadeh R. (2012). Allelic polymorphism of Makoei sheep calpastatin gene identified by polymerase chain reaction and single strand conformation polymorphism. J. Agric. Sci. Technol. 14, 533-538.
Salvetti N.R., Acosta J.C., Gimeno E.J., Müller L.A., Mazzini R.A., Taboada A.F. and Ortega H.H. (2007). Estrogen receptors alpha and beta and progesterone receptors in normal bovine ovarian follicles and cystic ovarian disease. Vet. Pathol. 44(3), 373-378.
SAS Institute. (2004). SAS®/STAT Software, Release 9.2. SAS Institute, Inc., Cary, NC. USA.
Shen W., Wisniowski P., Ahmed L., Boyle D.W. and DenneS.C. (2003). Protein anabolic effects of insulin and IGFI in the ovine fetus. Am. J. Physiol. Endocrinol. Metab. 284, 48-56.
Tahmoorespur M., Valeh M.V., Nassiry M.R., Heravi Moussavi A. and Ansary M. (2009). Association of the polymorphism in the 5’ flanking region of the ovine IGFI gene with growth traits in the Baluchi sheep. South African J. Anim. Sci. 39, 97-101.
Weller J.L. (2009). Quantitative Trait Loci Analysis in Animals. Centre for Agriculture and Biosciences International (CABI) Publications, Wallingford, UK.
Yilmaz A., DavisM.E. and Hines H.C. (2000). APCR-SSCP polymorphism detected in the 5 flanking regions of the ovine IGF1 gene. Research and reviews: beef and sheep. Spec. Circul. 5, 170-199.
Yilmaz A., Davis M.E., Hines H.C. and Chung H. (2005). Detection of two nucleotide substitutions and putative promoters in the 5’ flanking region of the ovine IGFI gene. J. Appl. Genet. 46, 307-309.