Genetic Variation of Cytochrome b Gene in Three Local Goat Breeds in Central Java of Indonesia
Subject Areas : Camelس. سوتوپو 1 , ا. پوربوواتی 2 , ا. کورنیانتو 3
1 - Department of Animal Production, Faculty of Animal and Agricultural Science, Diponegoro University, Tembalang Campus, Semarang, Indonesia
2 - Department of Animal Production, Faculty of Animal and Agricultural Science, Diponegoro University, Tembalang Campus, Semarang, Indonesia
3 - Department of Animal Production, Faculty of Animal and Agricultural Science, Diponegoro University, Tembalang Campus, Semarang, Indonesia
Keywords: genetic diversity, mitochondrial DNA, indigenous goat,
Abstract :
This study was conducted to elucidate the genetic diversity and phylogenic relationship of three local goat breeds in Central Java of Indonesia based on the cytochrome b (cyt b) gene sequence. Blood samples of 19, 15 and 12 heads of Kacang, Kejobong and Ettawa Grade breeds, respectively, were used as materials of the study. The multiple alignment of cyt b gene was conducted by comparing under study goat breeds and one deposited sequence in GeneBank (Capra hircus, accession number AB004072). Of the 16 sites of different nucleotide bases found, there were 12 sites that could be used as a marker to distinguish the three breeds of local goat in Indonesia with the Capra hircus. Genetic distance among local goats was quite close compared to genetic distance between those local goats to GenBank’s Capra hircus. Phylogeny tree showed two main clusters, that was cluster A for Capra hircus and cluster B for three local goat breeds of Indonesia.
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