The Effect of Cattle Seminal Plasma Crude Protein on the Cryopreservation of Goat Semen
Subject Areas : Camelت. وایو سوپرایوگی 1 , س. سوسیلواتی 2
1 - Department of Reproduction, Faculty of Veterinary, Airlangga University, Surabaya, East Java, Indonesia
2 - Department of Reproduction, Faculty of Veterinary, Airlangga University, Surabaya, East Java, Indonesia
Keywords: viability, cattle seminal plasma crude protein, cytochrome C, motility,
Abstract :
This study aimed to improve the quality of cryopreserved goat semen for artificial insemination by the addition of cattle seminal plasma crude protein. Semen collected from cattle and centrifuged to obtain seminal plasma. Then it was purified to obtain the crude protein. The diluent used in this study was milk-yolk. There were three groups, control group without crude protein (P0), the addition of 2.5 mg crude protein/mL diluent (P1); and the addition of 5 mg crude protein/mL diluent (P2). The results showed that P1 had the highest percentage of progressive motility and viability. Meanwhile, P1 had the lowest percentage of cytochrome C expression. The lowest cytochrome C expression indicates that the mitochondria have a good function. So, by the addition of 2.5 mg crude protein/mL diluent, the quality of frozen goat semen in terms of sperm motility, viability, and cytochrome C expression in the spermatozoa could be improved.
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