Association between Ovoinhibitor (OIH) Gene Polymorphism and Egg Quality Traits in Golden Kamper Hybrid Chickens (Gallus gallus domesticus)

Subject Areas : Camel

D. Retnosari ¹ , B.S. Daryono ²

1 - Genetics and Breeding Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan Sekip Utara, 55281, Indonesia
2 - Genetics and Breeding Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan Sekip Utara, 55281, Indonesia

Received: 2022-01-10 Accepted : 2022-04-29 Published : 2022-09-01

Keywords: chicken, polymorphism, egg quality, ovoinhibitor gene,

Abstract :

The ovoinhibitor (OIH) has been identified in all egg compartments including egg white, egg yolk, and eggshell. The objective of this study was to analyze the association between OIH gene polymorphism and egg quality in the Golden Kamper Hybrid chickens. The chickens used in this study were BC Golden Kamper and F4 Golden Kamper. Chickens were maintained in the individual cage to observe egg quality. Chicken blood samples were taken for DNA isolation by Chelex 5% method followed by OIH gene amplification and polymorphism detection with sanger sequencing. Significant differences in egg quality were analyzed using one-way ANOVA. The relationship between genotype and haplotype with egg quality was analyzed using Pearson correlation. Average egg weight, egg length, shell weight, and shell thickness of BC Golden Kamper were 47.06 ± 2.91 g, 5.13 ± 0.3 mm, 4.58 ± 0.52 g, 0.30 ± 0.03 mm and F4 Golden Kamper was 47.42 ± 3.74 g, 5.16 ± 0.2 mm, 5.18 ± 0.33 g and 0.315 ± 00 mm. The mean albumen height, haugh unit, and yolk color of BC Golden Kamper was 5.4 ± 1.14 mm, 85.86 ± 8.71, 8.80 ± 0.45 and F4 Golden Kamper was 6.13 ± 0.33 mm, 79.42 ± 2.21, 8.00 ± 0. There was a single nucleotide polymorphisms (SNP) G4363T in the OIH gene intron 7. Genotype (GG and TT) and haplotype (H1 and H2) were significantly negatively correlated with albumen weight (P<0.05), shell weight (P<0.05), and shell thickness (P<0.05).

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