Effects of Oregano (Origanum vulgare) and Thyme (Thymus vulgaris) Oils on Growth Performance and Blood Parameters in Holstein Suckling Calves
Subject Areas : Camel
1 - Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2 - Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Keywords: growth performance, blood metabolites, calf, oregano oil, thyme oil,
Abstract :
This experiment was conducted to compare the effect of supplementation of milk with thyme (THY) and oregano (ORE) essences separately or a mixture of THY and ORE essences on growth performance, haematological parameters and some blood metabolites of Holstein suckling calves. Forty-eight Holstein calves (48.31±5.82 kg) were randomly allocated to four treatments including (1) control without essential oil supplement; (2) THY oil mixed in milk at 5 mL/d/calf; (3) ORE oil mixed in milk at 5 mL/d/calf; (4) 2.5 mL THY oil + 2.5 mL/d/calf ORE oil mixed in milk (TOM). According to the results, the experimental diets had no significant effect on body weight, average daily gain, and feed conversion rate in the entire experiment (P>0.05), although the parameters of starter and total feed intake were increased (P<0.0001) in the ORE and TOM groups. Values of packed cell volume, haemoglobin, mean corpuscular volume, and lymphocyte percentage were increased (P<0.05) in the ORE group compared with the control. Also, our results demonstrate the supplementation of either oregano or thyme essential oils increased (P<0.05) serum iron concentration, alanine aminotransferase, and aspartate transaminase activities. Concentrations of total cholesterol and total triglyceride were decreased (P<0.05) in calves given both oils compared to the control group. Further confirmation of these results is warranted, and more research is needed to identify and clearly explain in the contribution of oregano or thyme oils (individual or mixtures); however our results suggested that oregano oil might be more useful as the promising feed additive for suckling calves nutrition.
Alcicek A., Bozkurt M. and Cabuk M. (2003). The effect of an essential oil combination derived from selected herbs growing wild in Turkey on broiler performance. South Africa J. Anim. Sci. 33, 89-94.
Alsaht A.A., Bassiony S.M., Abdel Rahman G.A. and Shehata S.A. (2014). Effect of cinnamaldehyde thymol mixture on growth performance and some ruminal and blood constituents in growing lambs fed high concentrate diet. Life Sci. J. 11, 240-248.
AOAC. (2005). Official Methods of Analysis. Vol. I. 18th Ed. Association of Official Analytical Chemists, Arlington, VA, USA.
Bampidis V.A., Christodoulou V., Florou-Paneri P., Christaki E., Spais A.B. and Chatzopoulou P.S. (2005). Effect of dietary dried oregano leaves supplementation on performance and carcass characteristics of growing lambs. Anim. Feed Sci. Technol. 121, 285-295.
Baranauskiene R., Venskutonis P.R., Viskelis P. and Dambrauskiene E. (2003). Influence of nitrogen fertilizers on the yield and composition of thyme (Thymus vulgaris). J. Agric. Food Chem. 51, 7751-7758.
Benchaar C., Calsamiglia S., Chaves A.V., Fraser G.R., Colombatto D., McAllister T.A. and Beauchemin K.A. (2008). A review of plant-derived EO in ruminant nutrition and production. Anim. Feed Sci. Technol. 145, 209-228.
Calsamiglia S., Busquet M., Cardozo P., Castillejos L. and Ferret A. (2007). Essential oils as modifiers of rumen microbial fermentation: a review. J. Dairy Sci. 90, 2580-2595.
Cardozo P.W., Calsamiglia S., Ferret A. and Kamel C. (2006). Effects of alfalfa extract, anise, capsicum, and a mixture of cinnamaldehyde and eugenol on ruminal fermentation and protein degradation in beef heifers fed a high-concentrate diet. J. Anim. Sci. 84, 2801-2808.
Chaves A.V., Dugan M.E.R., Stanford K., Gibson L.L., Brystom J.M., McAllister T.A., Van Herk F. and Benchaar C. (2011). A dose – response of cinnamaldehyde supplementation on intake, ruminal fermentation, blood metabolites, growth performance, and carcass characteristics of growing lambs. Livest. Sci. 141, 213-220.
Chaves A.V., Stanford K., Dugan M.E.R., Gibson L.L., McAllister T.A., Van Herk F. and Benchaar C. (2008). Effects of cinnamaldehyde, garlic and juniper berry essential oils on rumen fermentation, blood metabolites, growth performance, and carcass characteristics of growing lambs. Livest. Sci. 117, 215-224.
Davidson P.M. (2001). Chemical preservatives and naturally antimicrobial compounds. Pp. 593-627 in Food Microbiology: Fundamentals and Frontiers. M.P. Beuchat and L.R. Montville, Eds. ASM Press, Washington, DC, USA.
Davidson S., Hopkins B.A., Diaz D.E., Bolt S.M., Brownie C., Fellner V. and Whitlow L.W. (2003). Effects of amounts and degradability of dietary protein on lactation, nitrogen utilization, and excretion in early lactation Holstein cows. J. Dairy Sci. 86, 1681-1689.
Elson C.E. and Qureshi A.A. (1995). Coupling the cholesterol- and tumor-suppressive actions of palm oil to the impact of its minor constituents on 3-hydroxy-3-methylglutaryl coenzyme A reductase activity. Prostaglandins Leukot. Essent. Fatty Acids. 52, 205-208.
European Commission. (2003). Regulation (EC). No. 1831/2003 of the European Parliament and of the Council of 22 September 2003 on additives for use in animal nutrition. Off. J. European Union. 268, 29-43.
Exarchou V., Nenadis N., Tsımıdou M., Gerothanassis I.P., Troganis A. and Boskou D. (2002). Antioxidant activities and phenolic composition of extracts from greek oregano, greek sage, and summer savory. J. Agric. Food Chem. 50, 5294-5299.
Fathi M.H., Riasi A. and Allahresani A. (2009). The effect of vanilla flavoured calf starter on performance of Holstein calves. J. Anim. Feed Sci. 18, 412-419.
Franchini M., Salvagno G.L., Montagnana M. and Lippi G. (2007). Serum ferritin levels correlate with haemoglobin concentration: a report on 589 outpatients from a single centre. Blood Transfus. 5(4), 244-245.
Geraci J.I., Garciarena A.D., Gagliostro G.A., Beauchemin K.A. and Colombatto D. (2012). Plant extracts containing cinnamaldehyde, eugenol and capsicum oleoresin added to feedlot cattle diets: ruminal environment, short term intake pattern and animal performance. Anim. Feed Sci. Technol. 176, 123-130.
Jain N.C. (1998). Essentials of Veterinary Hematology. Lea and Febiger Publication, Philadelphia, USA.
Manzanilla E.G., Perez J.F., Martin M., Kamel C., Baucells F. and Gasa J. (2004). Effect of plant extracts and formic acid on the intestinal equilibrium of early weaned pigs. J. Anim. Sci. 82, 3210-3218.
Marino M., Bersani C. and Comi G. (2001). Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. Int. J. Food Microbiol. 67, 187-195.
Morrill J.L. and Dayton A.D. (1978). Effect of feed flavor in milk and calf starter on feed consumption and growth. J. Dairy Sci. 61, 229-232.
Ncube N., Afolayan S.A.J. and Okoh A.I. (2008). Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. African J. Biotechnol. 7(12), 1797-1806.
Nostro A. and Papalia T. (2012). Antimicrobial activity of carvacrol: current progress and future prospectives. Recent Pat. Antiinfect. Drug Discov. 7, 28-35.
NRC. (2001). Nutrient Requirements of Dairy Cattle. 7th Ed. National Academy Press, Washington, DC, USA.
Özguven M. and Tans S. (1998). Drug yield and essential oil of Thymus vulgaris as in influenced by ecological and on to genetical variation. Turkish J. Agric. For. 22, 537-542.
Petit H.V. and Flipot P.M. (1992). Feed utilization of beef steers fed grass as hay or silage with or without nitrogen supplementation. J. Anim. Sci. 70, 876-883.
Santos F.H.R., De Paula M.R. and Lezier D. (2015). Essential oils for dairy calves: effects on performance, scours, rumen fermentation and intestinal fauna. Animal. 9(6), 958-965.
Savoini G., Bontempo V., Cheli F., Baldi A., Sala V., Mancin G., Agazzi A. and Dell'Orto V. (2002). Alternative antimicrobials in the nutrition of postweaning piglets. Vet. Rec. 151(19), 577-580.
SAS Institute. (2004). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Thomas L.C., Wright T.C., Formusiak A., Cant J.P. and Osborne V.R. (2007). Use of flavored drinking water in calves and lactating dairy cattle. J. Dairy Sci. 90, 3831-3837.
Vakili A.R., Khorrami B., Danesh Mesgaran M. and Parand E. (2013). The effects of thyme and cinnamon essential oils on performance, rumen fermentation and blood metabolites in Holstein calves consuming high concentrate diet. Asian Australasian J. Anim. Sci. 26(7), 935-944.
Yang W.Z., Benchaar C., Ametaj B.N. and Beauchemin K.A. (2010). Dose response to eugenol supplementation in growing beef cattle: ruminal fermentation and intestinal digestion. Anim. Feed Sci. Technol. 158, 57-64.