In vitro Maturation and Fertilization of Buffalo Oocytes Cultured in Media Supplemented with Bovine Serum Albumin
Subject Areas : Camelآ.ان.ام.ای. رحمان 1 , ام.آ.ام.ی. خاندوکر 2 , ال. اسد 3 , اس. ساها 4 , ر.س. پاوول 5 , اس. دبناس 6
1 - Department of Animal Nutrition, Genetics and Breeding, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh
2 - Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
3 - Department of Animal Nutrition, Genetics and Breeding, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh
4 - Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
5 - Department of Genetics and Animal Breeding, Patuakhali Science and Technology University, Babugong, Barisal, Bangladesh
6 - Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
Keywords: Buffalo, BSA, cumulus-oocyte-complexes, IVF, IVM,
Abstract :
The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the maturation medium supplemented with 5% of BSA, the other without supplementation (control). Matured oocyte fertilized with capacitated frozen-thawed semen in Brackett and Oliphant (BO) medium for 5 hours, in an incubator at 38.5 °C with 5% CO2 underhumidified air. A significantly higher number of normal quality COCs per ovary (P<0.05) were obtained from ovaries devoid of corpus luteum (CL) compared to ovaries having CL (1.84 vs. 0.81) respectively. The percentage of oocytes reaching Metaphase-II (M-II) stages was 58.07±2.08 and 68.10±0.75% for control and 5% level of BSA respectively. The fertility level was assessed by pronuclei formation: the normal fertilization rate (2PN) obtained was 19.63±3.11 and 29.52±1.98% for control and BSA supplementation respectively. Significant differences (P<0.05) were observed in maturation (M-II) and fertilization (2PN) rate of buffalo oocyte by adding 5% level of BSA supplementation in culture media. Thus, data gathered in this study showed that 5% BSA supplementation in both maturation and fertilization media can be used for enhance the maturation and fertilization rate of buffalo oocytes, as well as to improve the grade of collected buffalo COCs.
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