In vitro Maturation and Fertilization of Buffalo Oocytes Cultured in Media Supplemented with Bovine Serum Albumin

Subject Areas : Camel

آ.ان.ام.ای. رحمان ¹ (Department of Animal Nutrition, Genetics and Breeding, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh)
ام.آ.ام.ی. خاندوکر ² (Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh)
ال. اسد ³ (Department of Animal Nutrition, Genetics and Breeding, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh)
اس. ساها ⁴ (Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh)
ر.س. پاوول ⁵ (Department of Genetics and Animal Breeding, Patuakhali Science and Technology University, Babugong, Barisal, Bangladesh)
اس. دبناس ⁶ (Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh)

Received: 2014-11-07 Accepted : 2015-01-31 Published : 2015-09-01

Keywords: Buffalo, BSA, cumulus-oocyte-complexes, IVF, IVM,

Abstract :

The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the maturation medium supplemented with 5% of BSA, the other without supplementation (control). Matured oocyte fertilized with capacitated frozen-thawed semen in Brackett and Oliphant (BO) medium for 5 hours, in an incubator at 38.5 °C with 5% CO2 underhumidified air. A significantly higher number of normal quality COCs per ovary (P<0.05) were obtained from ovaries devoid of corpus luteum (CL) compared to ovaries having CL (1.84 vs. 0.81) respectively. The percentage of oocytes reaching Metaphase-II (M-II) stages was 58.07±2.08 and 68.10±0.75% for control and 5% level of BSA respectively. The fertility level was assessed by pronuclei formation: the normal fertilization rate (2PN) obtained was 19.63±3.11 and 29.52±1.98% for control and BSA supplementation respectively. Significant differences (P<0.05) were observed in maturation (M-II) and fertilization (2PN) rate of buffalo oocyte by adding 5% level of BSA supplementation in culture media. Thus, data gathered in this study showed that 5% BSA supplementation in both maturation and fertilization media can be used for enhance the maturation and fertilization rate of buffalo oocytes, as well as to improve the grade of collected buffalo COCs.

References:

Amer H.A., Hegab A.O. and Zaabal S.M. (2008). Effects of ovarian morphology on oocyte quantity and quality, granulosa cells, in vitro maturation, and steroid hormone production in buffaloes. J. Anim. Reprod. 5, 55-62.

Anand T., Kumar D., Chauhan M.S., Manik R.S. and Palta P. (2008). Cysteamine supplementation on in vitro maturation medium, in vitro culture medium or both media promotes in vitro development of buffalo (Bubalus bublis). Reprod. Fertil. Dev. 20, 253-257.

Apu A.S., Khandoker M.A.M.Y., Husain S.S., Fkruzzaman M. and Notter D.R. (2012). A comparative study of fresh and frozen-thowed semen quality in relation to fertility of Black Bengal goats. Iranian J. Appl. Anim. Sci. 2 (2), 157-161.

Ball G.D., Leibried M.L., Ax R.L. and First N.L. (1984). Maturation and fertilization of bovine oocytes in vitro. J. Dairy Sci. 67, 2775-2785.

BER. (2012). Bangladesh Economic Review. Finance Division, Ministry of Finance, Government of Bangladesh.

Bracket B.G. and Oliphant G. (1975). Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. 12, 260- 274.

Bavister B.D., Hellekant T.A.R. and Pinnyomminter T.(1992). Development of in vitro and matured in vitro fertilized bovine embryos into morula and blastocyst in defined culture media. Theriogenology. 37, 127-146.

Drost M. (2007). Advanced reproductive technology in the water buffalo. Theriogenology. 68, 450-453.

Faruque M.O., Hasnath M.A. and Siddique N.U. (1990). Present status of buffaloes and their productivity. Asia-australs J. Anim. Sci. 3, 287-292.

Farin P.W., Crozier A.E. and Farin C.E. (2001). Influence of in vitro system on embryo survival and detail development in cattle. Theriogenology. 55, 151-170.

Fuki Y. and Ono H. (1989). Effect of sera, hormones and granulosa cells added to culture media for in vitro maturation, fertilization, cleavage and development of bovine oocytes. J. Reprod. Fertil. 86, 501-506.

Galli C. and Lazzari G. (2003). Achievements of research in the field of Reproduction Science. Istituto Sperimentale Italiano Lazzaro Spallanzani, Via Porcellasco 7/F, 26100 Cremona Italia.

Gasparrini B., Boccia L., De Rosa A., Vecchio D., Di Palo R. and Zicrelli L. (2004). In vitro fertilization of buffalo (Bubalus bubalis) oocytes: effects of media and sperm motility inducing agents. Reprod. Fertil. Dev. 16 (2), 255-256.

Gordon I. (1994). Laboratory Production of Cattle Embryo. CAB International, Wallingford, Oxon OX 108 DE, UK.

Hammam A.M., Whisnant C.S., Elias A., Zaabel S.M., Hegab A.O. and Abu-El Naga E.M. (2010). Effect of media, sera and hormones on in vitro maturation and fertilization of Water Buffalos (Bubalus bubalis). J. Anim. Vet. Adv. 9, 27-31.

Hafez E.S.E. (1993). Reproduction in Farm Animals. Lea and Febiger. Philadelphia.

Hoque S.A.M., Kabiraj S.K., Khandoker M.A.M.Y., Mondal A. and Tareq K.M.A. (2011). Effect of collection techniques on cumulus oocyte complexes (COCs) recovery, in vitro maturation and fertilization of goat oocytes. African J. Biotechnol. 10, 9177-9181.

Jamil H., Samad A., Qureshi Z.I., Rehman N.U. and Lodhi L.A. (2008). Harvesting and evaluation of riverine buffalo follicular oocytes. Turkish J. Vet. Anim. Sci. 32, 25-30.

Khandoker M.A.M.Y., Imai K., Takahashi T. and Hashizume K. (2001). Role of gelatinase on follicular atresia in the bovine ovary. Biol. Reprod. 65, 720-732.

Khandoker M.A.M.Y., Jahan N., Asad L., Hoque S.A.M., Ahmed S. and Faruque M.O. (2011). Qualitative and quantitative analysis of buffalo ovaries, follicles and oocytes in view of in vitro production of embryos.Bangladesh J. Anim. Sci. 40 (1& 2), 23-27.

Khandoker M.A.M.Y., Reza M.M.T., AsadL.Y., SahaS., Apu A.S. and Hoque S.A.M. (2012). In vitromaturation of buffalo oocytes and fertilization by cattle spermatozoa. Bang. J. Anim. Sci.. 41 (1), 6-12.

Krisher R.L. (2004). The effects of oocyte quality on development. J. Anim. Sci. 82, 14-23.

Kumar D., Palta P., Manik R.S., Singla S.K. and Chauhan M.S. ( 2007). Effect of culture media and serum supplementation on the development of in vitro buffalo embryos. Indian. J. Anim. Sci. 77, 697-701.

Mahesh Y.U., Rao M.M., Sudhakar P. and Rao K.R.S.S. (2014). Effect of harvesting technique and presence or absence of corpus luteum on in vitro development after parthenogenetic activation of oocytes recovered from buffalo ovaries. Vet. World. 7, 315-320.

Mahmoud G.M. and Nawito M.F. (203). Cytogenetic evaluation of in vitro matured buffalo oocytes in different culture condition. Egyptian J. Vet. Sci. 37, 105-116.

Nandi S., Raghu H.M., Ravindranatha B.M. and Chauhan M.S. (2002). Production of buffalo (Bubalus bubalis) embryos in vitro: premises and promises. Reprod. Domest. Anim. 37, 65-74.

Neglia G., Gasparrini B., Caracciolo di Brienza V., Di Palo R., Campanile G., Presicce G.A., Zicarelli L. (2003). Bovine and buffalo in vitro embryo production using oocytes derived from abattoir ovaries or collected by transvaginal follicle aspiration. Theriogenology. 59, 1123-1130.

Sahoo L. and Singla S.K. (2013). Effects of corpus luteum: quality and recovery of buffalo (Bubalus bubalis) oocytes. Turkish J. Vet. Anim. Sci. 37, 468-471.

Seaton A.D., Catt J.W., Rhodes S.L., McDonald M.F. and Welch R.A.S. (1991). The use of unfrozen semen for in vitro fertilization of in vitro matured bovine oocytes. Proc. New Zealand Soci. Anim. Prod. 51, 67-71. 

Singh S., Dhanda O.P. and Malik R.K. (2001). Effects of the presence of corpus luteum on oocyte recovery and subsequent in vitro maturation and fertilization in buffaloes. Asian-australas J. Anim. Sci. 14, 1675-1677.

Sirard M.A. and Lmbert R.D. (1985). In vitro fertilization of bovine follicular oocytes obtained by laparoscopy. Biol. Reprod. 3, 487-494.

Stubbings R.B. and Wosik C.P. (1991). Glass wool versus swim-up separation of bovine spermatozoa for in vitro fertilization. Theriogenology. 35, 276.

Totey S.M., Singh G., Taneja. M., Pawshe C.H. nd Talwar G.P. (1992). In vitro maturation, fertilization and development of follicular oocytes from buffalo (Bubalus bubalis). J. Reprod. Fertil. 95, 597-607.

Wang Z.G., Yu S.D. and Xu S.R. (2007). Effects of collection methods on recovery efficiency, maturation rate and subsequent embryonic developmental competence of oocytes in Holstein cow. Asian-australs J. Anim. Sci. 20, 496-500.

Wani N.A., Wani G.M., Khan M.Z. and Salahudin S. (2000). Effect of oocyte harvesting techniques on in vitro maturation and in vitro fertilization in sheep. Small Rumi. Res. 36, 63-67.

Webb R., Campbell B.K., Garveric H.A. and Gong J.G. (1999). Molecular mechanisms regulating follicular recruitment and selection. J. Reprod. Fertil. 45, 123-126.