Effect of Pelleting Temperature, Probiotic and Wheat Grain on Growth Performance, Blood Biochemical Variables, Immune Responses and Mucin 2 Gene Expression of Broiler Chicks
Subject Areas : Camelو. ایقانی 1 , ع.ا. صادقی 2 , س.ن. موسوی 3 , پ. جعفری 4 , م. چمنی 5
1 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Animal Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
4 - Department of Microbiology, Arak Branch, Islamic Azad University, Arak, Iran
5 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
Keywords: broiler chicks, Cholesterol, immune responses, mucin 2,
Abstract :
Conditioning is a major stage in the pelleting process for feeds. Probiotics are known to have the ability for improvement of the immune system and growth performance. Wheat can be used as a replacement for corn. Thus, the present study was conducted to investigate the effects of pelleting temperature, probiotics and wheat grain on growth performance, blood biochemical variables, immune responses and mucin 2 gene expression of broiler chicks. Eight-hundred 1-d-old broiler chicks were assigned into 8 treatments with 4 replicates (n=25 chicks). A completely randomized design in a 2 × 2 × 2 factorial arrangement was used with two levels of wheat (0 and 500 g/kg), two levels of probiotic (0 and 200 mg/kg) and two levels of conditioning temperature (70 or 85 ˚C). Growth performance, immune responses, blood biochemical variables, and mucin 2 gene expression were evaluated. Main and interaction effects were not significant on growth performance, immune responses and blood biochemical variables (P>0.05). Wheat and corn-based diets containing probiotic up-regulated mucin 2 gene expression (p < 0.05). Wheat can be used as an alternative for corn.
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