Influence of Fibrolytic Enzymes on the in vitro Hydrolysis and Fermentation of Different Types of Roughages Treatment
Subject Areas : Camel
1 - Department of Animal Science, FacultyofAgricultur, Shahrekord University, Shahrekord, Iran
Keywords: alkaline treatment, fibrolytic enzymes, roughages, <i>in vitro</i> degradation,
Abstract :
The effects of pre-treating different types of roughages with alkali on the efficacy of exogenous fibrolytic enzymes for improving their digestibility were studied in vitroin factorial arrangement 4 × 3 × 5 (enzyme, treatment and roughage types). Two fibrolytic feed enzymes novozyme (N) and celloclast (C) their combination (N+C) were evaluated for their potential to improve in vitrodegradation of different roughages including two straws (wheat straw: WS; barley straw: BS), grass hay (GH), corn stover (CS) and corn cobs (CC) as untreated or alkaline treated (NaOH or urea). The enzyme products were in liquid form and applied at a rate of 1 mg enzyme protein/g DM substrate. Anaerobic buffer medium and strained ruminal fluid were added to the in vitroincubations. Degradabilities of dry matter (DM), neutral detergent fibre (NDF), cellulose, soluble protein and reducing sugar profiles were determined at the end of the 24 h incubation. Overall NDF degradability of roughages were slightly increased by alkaline treatment (P<0.05) but, the addition of fibrolytic enzymes directly to the ruminal fluid at the application rates could not increase significantly DM or cellulose degradation (P>0.05). Use of fibrolytic enzymes released higher amounts of reducing sugars and lower soluble protein compared to control (P<0.05). Alkali treatment increased (P<0.05) the degradability of DM and fiber degradability. It was higher in NaOH treated forage compared to urea treated forages (P<0.05).
Arakawa T. and Timasheff S.N. (1984). Protein stabilization and destabilization by guanidinium salts. Biochemistry. 23, 5924-5929.
Beauchemin K.A., Colombatto D., Morgavi D.P. and Yang W.Z. (2003). Use of exogenous fibrolytic enzymes to improve feed utilization by ruminants. J. Anim. Sci. 81, 37-47.
Cline T.R., Garrigus U.S. and Hatfield E.E. (1966). Addition of branched and straight chain volatile fatty acids to purified lamb diets and effects on utilization of certain dietary components. J. Anim. Sci. 25, 734-739.
Dias Da Silva A., Mascarenhas Ferreira A. and Guedes C.V.M. (1988). Effects of moisture level, treatment time and soyabean addition on the nutritivevalue of urea treated maize stover. Anim. Feed Sci. Technol. 19, 67-77.
Euna J.S. Beauchemin K.A. Hong S.H. and Bauer M.W. (2006). Exogenous enzymes added to untreated or ammoniated rice straw: Effects on in vitro fermentation characteristics and degradability. Anim. Feed Sci. Technol. 131, 86-101.
FAO. (2002). Agricultural Statistics. Food and Agriculture Organization of the United Nations,Rome. http://apps1.fao.org.
Lee S.S., Ha J.K. and Cheng K.J. (2000). Relative contributions of bacteria, protozoa, and fungi to in vitro degradation of orchard grass cell walls and their interactions. Appl. Environ. Microb. 66, 3807-3813.
Liu J.X. and Orskov E.R. (2000). Cellulase treatment of untreated and steam pre-treated rice straw effect on in vitro fermentation characteristics. Anim. Feed Sci. Technol. 88, 189-200.
Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J. (1951). Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265-275.
Mertens D.R. (2002). Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: collaborative study. J. AOAC. Int. 85, 1217-1240.
Miller G.L. (1959). Use of dinitrosalicilic acid reagent for determination of reducing sugar. Anal. Chem. 31, 426-428.
Moharrery A., Hvelplund T. and Weisbjerg M.R. (2009). Effect of forage type, harvesting time and exogenous enzyme application on degradation characteristics measured using in vitro technique. Anim. Feed Sci. Technol. 153, 178-192.
SAS Institute. (2009). User’s Guide: Statistics, Version 9.2 Edition. SAS Inst., Inc., Cary, NC, USA.
Taiwo A.A., Adebowale E.A., Greenhalgh J.F.D. and Akinsoyinu A.O. (1995). Techniques for trapping ammonia generated from urea treatment of barley straw. Anim. Feed Sci. Technol. 56, 133-141.
Tuah A.K. and Ørskov E.R. (1987). The degradation of untreated and treated maize cobs and cocoa pod husks in the rumen. Pp 363-378 in: Overcoming Constraints to the Efficient Utilization of Agricultural by-Products as Animal Feed. International Livestock Research Institute, Ed. ILRI (aka ILCA and ILRAD).
Turner M.B., Spear S.K., Huddleston J.G., Holbrey J.D. and Rogers R.D. (2003). Ionic liquid salt induced inactivation and unfolding of cellulose from Trichoderma reesei.Green Chem. 5,443-447.
Van Soest P.J. (1982). Nutritional Ecology of the Ruminant. Corvallis, Oregon.
Wang Y., Spratling B.M., Wiedmeier D.R. and McAllister T.A. (2004). Effect of alkali pretreatment of wheat straw on the efficacy of exogenous fibrolytic enzymes. J. Anim. Sci. 82, 198-208.
Williams P.E.V., Innes G.M. and Brewer A. (1984). Ammonia treatment of straw via the hydrolysis of urea (II). Addition of soyabean (urease), sodium hydroxide and molasses: effects on the digestibility of urea treated straw. Anim. Feed Sci. Technol. 11, 115-124.