Identification of Pseudohermaphrodite Polled Apparently Female Goats Based on ZFX and SRY Sex Determination
Subject Areas : CamelH. Abyar 1 , B. Qasemi-Panahi 2 , G. Moghaddam 3 , S.A. Rafat 4 , A. Javanmard 5
1 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
4 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
5 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Keywords: Goat, intersexuality, polled intersex syndrome, <i>SRY</i>, <i>ZFX</i>,
Abstract :
The presence of intersexuality in goat breeds is one of the most popular, and challenging issues. Therefore, understanding the genetic complexity of this phenomenon at the DNA level may help to discover the molecular mechanisms of sexual differentiation in this species. A solution to this problem is proposed in the development of a DNA test using sex-determination candidate genes. With this motivation, the main goal of the present report was to identify the pseudohermaphrodite polled goats based on ZFX and SRY sex-determination candidate gene findings. Overall, data were collected from 11 individuals (4 females, 4 males, and 3 anatomically female goats (intersex) were chosen for PCR analysis using ZFX and SRY specific primers sequence. For this reason, genomic DNA extraction and PCR amplification were done according to routinely available laboratory protocol. Two ZFX and SRY-specific primer sets were applied for PCR amplification. Interestingly, our results were re-confirmed, and PCR products were amplified by ZFX primer yielding a band of 445 bp in both female and intersex goats. Furthermore, SRY expressed 169 bp in both male and intersex goats. In summary, these results suggest that SRY candidate gene may provide insight into the factors affecting the intersexuality issue in goat breeds.
Abbas N.A., Toublanc J.E., Boucekkine G., Toublanc M., Affara N.A., Job J.C. and Fellous M. (1990). A possible common origin of negative' human XX males and XX true hermaphrodites. Hum. Genet. 84, 356-360.
Aasen E. and Medrano J.F. (1991). Amplification of the Zfy and Zfx genes for sex identification in humans, cattle, sheep and goats. Biotechnology. 8, 1279-1281.
Bosu W.T.K. and Basrur P.K. (1984). Morphological and hormonal features of an ovine and a caprine intersex. Canadian J. Compar. Med. 48, 402-409.
Cribiu E.P. and Chaffaux S. (1990). Intersexuality in domestic mammals. Reprod. Nutr. Dev. 1, 51-61.
Dhanoa J.K., Mukhopadhyay C.S. and Arora J.S. (2016). Y-chromosomal genes affecting male fertility. Vet. World. 9(7), 783-795.
Eaglesome M.D., Hare W.C.D. and Singh E.L. (1979). Studies on obtaining meiotic chromosomes for analysis in the bull. I. Testicular biopsy. Theriogenology. 12(5), 263-270.
Fábián R., Kovács A., Stéger V., Frank K., Egerszegi I., Oláh J. and Bodó S. (2017). X-and Y-chromosome-specific variants of the amelogenin gene allow non-invasive sex diagnosis for the detection of pseudohermaphrodite goats. Hungarica Act. Vet. 65(4), 500-504.
Galan-Caridad J.M., Harel S., Arenzana T.L., Hou Z.E., Doetsch F.K., Mirny L.A. and Reizis B. (2007). ZFX controls the self-renewal of embryonic and hematopoietic stem cells. Cell. 129(2), 345-357.
Graves J.A.M. (2015). Twenty-five years of the sex-determining gene: The discovery that the gene SRY on the mammalian Y chromosome drives testis development marked a turning point in the decades-long quest to understand the genetic underpinnings and evolution of sex determination. Nature. 582(7582), 343-345.
Hafez S.A., Huckle W.R. and Caceci T. (2005). Anatomical, histological and genetic investigations of a sexually anomalous goat. Vet. Rec. 157(17), 513-516.
Hamerton J.L., Dickson J.M., Pollard C.E., Grieves S.A. and Short R.V. (1969). Genetic intersexuality in goats. J. Reprod. Fertil. 7, 25-51.
He Y., Zhang Y., Li X., Zhou R., Li L. and Wang Z. (2019). SRY and DelE detection in polled intersex Tangshan dairy goat. Indian J. Anim. Res. 53(7), 860-863.
Hunter R.H.F. (1996). Aetiology of intersexuality in female (XX) pigs, with novel molecular interpretations. Mol. Reprod. Dev. 45, 392-402.
McElreavey K., Vilain E., Abbas Herskowitz I. and Fellous M. (1993). A regulatory cascade hypothesis for mammalian sex determination: SRY represses a negative regulator of male development. Proc. Natl. Acad. Sci. USA. 90, 3368-3372.
Montgomery G.W., Henry H.M., Dodds K.G., Beattie A.E., Wuliji T. and Crawford A.M. (1996). Mapping the Horns (Ho) locus in sheep: A further locus controlling horn development in domestic animals. J. Hered. 87(5), 358-363.
Pailhoux E., Cribiu E.P., Chaffaux S., Darre R., Fellous M. and Cotinot C. (1994). Molecular analysis of 60, XX pseudohermaphrodite polled goats for the presence of SRY and ZFY genes. J. Reprod. Fertil. 100, 491-496.
Palmer M.S., Berta P., Sinclair A.H., Pym B. and Goodfellow P.N. (1990). Comparison of human ZFY and ZFX transcripts. Proc. Natl. Acad. Sci. 87(5), 1681-1685.
Pannetier M., Elzaiat M., Thépot D. and Pailhoux E. (2012). Telling the story of XX sex reversal in the goat: highlighting the sex-crossroad in domestic mammals. Sex. Dev. 6(1), 33-45.
Petit C., de la Chapelle A., Levilliers J., Castillo S., Noel B. and Weissenbach J. (1987). An abnormal terminal -Y interchange accounts for most but not all cases of human XX maleness. Cell. 49, 565-602.
Ramadan R.O. and El Hassan A. (1988). Intersexuality in goats. New Zealand J. Vet. 36(3), 120-124.
Ramadan R., Gameel A., Dafalla E. and Galil A. (1991). Bilateral scrotal hysterocele intersex goat. J. Vet. Med. A. 38, 441-444.
Seboun E., Leroy P., Casanova M., Magenis E., Boucekkine C., Disteche C. and Fellous M. (1986). A molecular approach to the study of the human Y chromosome and anomalies of sex determination in man. Cold Spring Harb. Symp. Quant. Biol. 51, 237-248.
Sekido R. (2010). SRY: A transcriptional activator of mammalian testis determination. Int. J. Biochem. Cell Biol. 42(3), 417-420.
Shams S.S., Vahed S.Z., Soltanzad F., Kafil V., Barzegari A., Atashpaz S. and Barar J. (2011). Highly effective DNA extraction method from fresh, frozen, dried and clotted blood samples. BioImpacts. 1, 183-195.
Song C.F.L.Y., Zhang D.X., Jiao L., Wang G.M. and Liu X.J. (2015). Anatomical observation of reproductive organs in intersex goats. Heilongjiang Anim. Sci. Vet. Med. 16, 81-91.
Strah R. and Kunej T. (2019). Molecular sexing assays in 114 mammalian species: In silico sequence reanalysis and a unified graphical visualization of diagnostic tests. Ecol. Evol. 9(8), 5018-5028.
Wang Y.Q. and Zhang X. (1993). Study on cytogenetics of intersex livestock. J. Zhengzhou Pastoral. Sci. 13, 20-23.
Xiao C., Li J., Xie T., Chen J., Zhang S., Elaksher S.H. and Du X. (2021). The assembly of caprine Y chromosome sequence reveals a unique paternal phylogenetic pattern and improves our understanding of the origin of domestic goat. Ecol. Evol. 11(12), 7779-7795.
Zhan T., Tian Y., Lou M., Liu J., Wang Y. and Zhao X. (1994). The genetic mechanism of intersexuality in milk goats of Saanen breed of Xinong. Acta Genet Sin. 21(5), 356-361.