Effects of Sodium Butyrate and Rosemary Leaf Meal on General Performance, Carcass Traits, Organ Sizes and Nutrient Digestibility of Broiler Chickens
Subject Areas : CamelM.C. Ogwuegbu 1 , C.E. Oyeagu 2 , H.O. Edeh 3 , C.E. Dim 4 , A.O. Ani 5 , F.B. Lewu 6
1 - Department of Animal Science, University of Nigeria Nsukka, Nsukka, Nigeria
2 - Department of Agriculture, Faculty of Applied Science, Cape Peninsula University of Technology, Wellington Campus, Private Bag X8, Wellington 7654, Cape Town, South Africa
3 - Department of Animal Science, University of Nigeria Nsukka, Nsukka, Nigeria
4 - Department of Animal Science, University of Nigeria Nsukka, Nsukka, Nigeria
5 - Department of Animal Science, University of Nigeria Nsukka, Nsukka, Nigeria
6 - Department of Agriculture, Faculty of Applied Science, Cape Peninsula University of Technology, Wellington Campus, Private Bag X8, Wellington 7654, Cape Town, South Africa
Keywords: Organic acids, nutrient digestibility, birds, Antibiotics, phytogenic,
Abstract :
The effect of sodium butyrate, rosemary meal and oxytetracycline supplementation on growth performance, carcass characteristics, visceral organs sizes and nutrient digestibility of broilers was investigated. A total of 320 one-day-old male “Arbor acres strain” broiler chicks were allotted to 10 dietary treatments with 4 replicates of 8 birds each. The treatments include: T1: basal diet (BD; negative control), T2: BD + 1 g/kg diet of oxytetracycline (positive control), T3: BD + 2 g sodium butyrate (SB)/kg diet, T4: BD + 4 g SB/kg diet, T5: BD + 2.5 g rosemary meal (RM)/kg diet, T6: BD + 5.0 g RM/kg diet, T7: BD + 2 g SB + 2.5 g RM/kg diet, T8: BD + 2 g SB + 5.0 g RM/kg diet, T9: BD + 4 g SB + 2.5 g RM/kg diet and T10: BD + 4 g SB + 5.0 g RM/kg diet. Birds fed T3, T4, and T5 had the highest (p <0.05) body weight with an improved feed conversion ratio. The dressed weight, breast weight and drumstick/thigh weight were better (p <0.05) for birds fed T3, T4 and T5. The weights of gizzard, liver and lungs were higher for birds fed T3, while, the length of small and large intestine were lower for birds fed T3, T4, and T5. The digestibility of the crude fiber, crude protein, crude fat and dry matter were higher (p <0.05) for birds fed T3, T4, and T5. It was concluded that, T3, T4, and T5 can be safely included for an improved performance; cut yields and nutrient digestibility. They also accelerated the development of health promoting (heart, kidney, and liver) and immune related (spleen) organs.
Abouelezz K., Abou-Hadied M., Yuan J., Elokil A., Wang G., Wang S., Wang J. and Bian G. (2019). Nutritional impacts of dietary oregano and Enviva essential oils on the performance, gut microbiota and blood biochemicals of growing ducks. Animal. 13(10), 2216-2222.
Abudabos H. and Yehia M. (2013). Effect of dietary mannan oligosaccharide from saccharomyces cerevisiae on live performance of broilers under clostridium perfringens challenge. Italian J. Anim. Sci. 12, 231-235.
Adil S., Banday T., Ahmad Bhat G., Salahuddin M., Raquib M. and Shanaz S. (2011). Response of broiler chicken to dietary supplementation of organic acids. J. Centr Europn Agric. 12(3), 498-508.
Adil S., Banday T., Bhat G.A., Mir M.S. and Rehman M. (2010). Effect of dietary supplementation of organic acids on performance, intestinal histomorphology, and serum biochemistry of broiler chicken. Vet. Med. Int. 10, 406-413.
Afsharmanesh M., Lotfi M. and Mehdipour Z. (2016). Effects of wet feeding and early feed restriction on blood parameters and growth performance of broiler chickens. Anim Nutr. 2, 168-172.
Aghazadeh A.M. and Tahayazdi M. (2012). Effect of butyric acid supplementation and whole wheat inclusion on the performance and carcass traits of broilers. South African J. Anim. Sci. 42, 241-248.
Ahsan U., Cengiz O., Raza I., Kuter E., Chacher M.F.A., Iqbal Z., Umar S. and Cakir S. (2016). Sodium butyrate in chicken nutrition: The dynamics of performance, gut microbiota, gut morphology, and immunity. World’s Poult. Sci. J. 72, 265-275.
Alagawany M., Elnesr S.S. and Farag M.R. (2019). Use of liquorice (Glycyrrhiza glabra) in poultry nutrition: Global impacts on performance, carcass and meat quality. World’s Poult. Sci. J. 75, 293-303.
Antongiovanni M., Buccioni A., Petacchi F., Leeson S., Minieri S., Martini A. and Cecchi R. (2007). Butyric acid glycerides in the diet of broiler chickens: Effects on gut histology and carcass composition. Italian J. Anim. Sci. 6(1), 19-25.
AOAC. (1990). Official Methods of Analysis. Vol. I. 15th Ed. Association of Official Analytical Chemists, Arlington, VA, USA.
Arbab S., Hafsa Z., Muhammad Y., Saima M., Asim A., Farina K., Saima A., Muhammad S.Y. and Habib R. (2017). Effect of sodium butyrate on performance, immune status, microarchitecture of small intestinal mucosa and lymphoid organs in broiler chickens. Asian-Australasian J. Anim. Sci. 30(5), 690-699.
Attia Y.A., Abd El-Hamid A.E., Ellakany H.F., Bovera F., Al-Harthi M.A. and Ghazaly S.A. (2013). Growing and laying performance of Japanese quail fed diet supplemented with different concentrations of acetic acid. Italian J. Anim. Sci. 12, 221-230.
Azza M.K. and Naela M.R. (2014). Effect of dietary supplementation of organic acids on performance and serum biochemistry of broiler chicken. Nat. Sci. 12(2), 38-45.
Behzad N., Ali Ahmad A.Q., Alireza S., Achille S. and Andres L.M.M. (2015). Effect of different dietary levels of rosemary (Rosmarinus officinalis) and yarrow (Achillea millefolium) on the growth performance, carcass traits and ileal micro-biota of broilers. Italian J. Anim. Sci.14(3), 3930-3939.
Brambilla T., Cenci F.F., Roberta G., Agostino M., Francesco R., Marco S. and Alberto L. (2000). Clinical and pharmacological profile in a clenbuterol epidemic poisoning of contaminated beef meat in Italy. Toxicol. Lett. 114(1), 47-53.
Cabuk M., Bozkurt M., Alcicek A., Akbas Y. and Kucukyilmaz K. (2006). Effect of a herbal essential oil mixture on growth and internal organ weight of broilers from young and old breeder flocks. South African J. Anim. Sci. 36, 135-141.
Carvalho I.T. and Santos L. (2016). Antibiotics in the aquatic environments: a review of the European scenario. Environ. Int. 94, 736-757.
Chamba F., Puyalto M., Ortiz A., Torrealba H., Mallo J.J. and Riboty R. (2014). Effect of partially protected sodium butyrate on performance, digestive organs, intestinal villi and E.coli development in broiler chickens. Int. J. Poult. Sci. 13(7), 390-396.
Dalólio F.S., Vaz D.P., Moreira J., Albino L.F.T. and Valadares L.R. (2015). Carcass characteristics of broilers fed enzyme complex. Biotechnol. Anim. Husband. 31, 153-162.
Dehghani-Tafti N. and Jahanian R. (2016). Effect of supplemental organic acids on performance, carcass characteristics, and serum biochemical metabolites in broilers fed diets containing different crude protein levels. Anim. Feed Sci. Technol. 211, 109-116.
Diarra M.S. and Malouin F. (2014). Antibiotics in Canadian poultry production and anticipated alternatives. Front. Microbiol. 5, 282-290.
Dibner J.J. and Buttin R.J. (2002) .Use of organic acids as a model to study the impact of Gut microflora in nutrition and metabolism J. Appl. Poult. Res. 11, 453-463.
Farag M.R. and Alagawany M. (2019). The role of Astragalus membranaceus as immune-modulator in poultry. World’s Poult. Sci. J. 75, 43-54.
Forgetta V., Rempel H., Malouin F., Vaillancourt J.R., Topp E. and Dewar K. (2012). Pathogenic and multidrug-resistant Escherichia fergusonii from broiler chicken. Poult. Sci. 91, 512-525.
Geetha V. and Chakavarthula S.N. (2018). Chemical composition and anti-inflammatory activity of Boswellia ovalifoliolata essential oils from leaf and bark. J. Forest. Res. 29, 373-381.
Gema N., Gaspar R. and Julián C. (2018). Antioxidant and Antimicrobial Properties of Rosemary (Rosmarinus officinalis): A Review. Medicines (Basel). 5(3), 98-113.
Ghazalah A.A. and Ali A.M. (2008). Rosemary leaves as a dietary supplement for growth in broiler chickens. Int. J. Poult. Sci. 7, 234-239.
Guilloteau P., Martin L., Eeckhaut V., Ducatelle R., Zabielski R.and Van Immerseel F. (2010). From the gut to the peripheral tissues: The multiple effects of butyrate. Nutr. Res. Rev. 23, 366-384.
Hassan H.M.A., Samy A., Amani A., Youssef W. and Mohamed M.A. (2018). Using different feed additives as alternatives to antibiotics growth promoter to improve growth performance and Carcass traits of broilers. Int. J. Poult. Sci. 17(6), 255-261.
Hoffman R.J., Robert J.R., Hoffman S., Freyberg C.L., Poppenga R.H. and Lewis S.N. (2001). Clenbuterol ingestion causing prolonged tachycardia, hypokalemia, and hypophosphatemia with confirmation by quantitative levels. J. Toxicol. Clin. Toxicol. 39(4), 339-344.
Jackson M.E., Geronian K., Knox A., McNabJ J. and McCartney E. (2004). A dose-response study with the feed enzyme β-mannanase in broilers provided with corn-soybean meal based diets in the absence of antibiotic growth promoters. Poult. Sci. 83, 1992-1996.
Jia W., Slominski B.A., Bruce H.L., Blank G., Crow G. and Jones O. (2009). Effects of diet type and enzyme addition on growth performance and gut health of broiler chickens during subclinical Clostridium perfringens challenge. Poult. Sci. 88, 132-140.
Jørgensen H., Zhao X.Q., Knudsen K.E.B. and Eggum B.O. (1996). The influence of dietary fibre source and level on the development of the gastrointestinal tract, digestibility and energy metabolism in broiler chickens. British J. Nutr. 75, 379-395.
Kaczmarek S.A., Barri A., Hejdysz M. and Rutkowski A. (2016). Effect of different doses of coated butyric acid on growth performance and energy utilization in broilers. Poult. Sci. 95, 851-859.
Kamboh A., Hang S., Khan M. and Zhu W. (2016). In vivo immunomodulatory effects of plant flavonoids in lipopolysaccharide challenged broilers. Animal. 10, 1619-1625.
Kummerer K. (2009). Antibiotics in the aquatic environmentea review part I. Chemosphere. 75, 417-434.
Landers T.F., Cohen B., Wittum T.E. and Larson E.L. (2012). A review of antibiotic use in food animals: perspective, policy, and potential. Public. Health Rep. 127, 4-22.
Lee J.Y., Chae B.J., Kim S.Y. and Lee J.H. (2008). Effect of dietary DDGS and mannanase supplementation on the growth performances, nutrients utilizability and immune response of broilers fed either high or low energy diet. Pp. 38-42 in Proc. 13th Anim. Sci. Congr. Asian-Australas. Assoc. Anim. Prod. Soc., Hanoi, Vietnam.
Leeson S., Namkung H., Antongiovanni M. and Lee E.H. (2005). Effect of butyric acid on the performance and carcass yield of broiler chickens. J. Poult. Sci. 84, 1418-1422.
Liu J.D., Bayir H.O., Cosby D.E., Cox N.A., Williams S.M. and Fowler J. (2017). Evaluation of encapsulated sodium butyrate on growth performance, energy digestibility, gut development, and Salmonella colonization in broilers. Poult. Sci. 96, 3638-3644.
Mansoub N.H. (2011). Comparison of effects of using Nettle (Utrica dioica) and probiotic on performance and serum composition of broiler chickens. Glob. Vet. 6, 247-250.
Mashayekhi H., Mazhari M. and Esmaeilipour O. (2018). Eucalyptus leaves powder, antibiotic and probiotic addition to broiler diets: Effect on growth performance, immune response, blood components and carcass traits. Animal. 12, 2049-2055.
Mnisi C.M., Matshogo-Niekerk V.R. and Mlambo V. (2017). Growth performance, haemo- chemical parameters and meat quality characteristics of male Japanese quails fed a Lippa javanica –based diet. South African J. Anim. Sci. 47, 661-667.
Moquet P.C.A., Onrust L., Van Immerseel F., Ducatelle R., Hendriks W.H. and Kwakkel R.P. (2016). Importance of release location on the mode of action of butyrate derivatives in the avian gastrointestinal tract. World's Poult. Sci. J. 72, 61-80.
Namkung H., Yu H., Gong J. and Leeson S. (2011). Antimicrobial activity of butyrate glycerides toward Salmonella typhimurium and Clostridium perfringens. Poult. Sci. 90, 2217-2222.
Nieto G., Díaz P., Bañón S. and Garrido M.D. (2010). Dietary administration of ewe diets with a distillate from rosemary leaves (Rosmarinus officinalis): Influence on lamb meat quality. Meat Sci. 84, 23-29.
Norouzi B., Ahmad A.A.Q., Seidavi A.S. and Marin A.L.M. (2015). Effect of different dietary levels of rosemary (Rosmarinus officinalis) and yarrow (Achillea millefolium) on the growth performance, carcass traits and ileal microbiota of broilers. Italian J. Anim. Sci. 14, 448-453.
NRC. (1994). Nutrient Requirements of Poultry, 9th Rev. Ed. National Academy Press, Washington, DC., USA.
Okonkwo W.I. and Akubuo C.O. (2007). Trombe wall system for poultry Brooding. Int. J. Poult. Sci. 6, 125-130.
Olukosi O.A., Sands J.S. and Adeola O. (2007). Supplementation of carbohydrases or phytase individually or in combination to diets for weanling and growing-finishing pigs. J. Anim. Sci. 85, 1702-1711.
Oyeagu C.E., Mlambo V. and Muchenje V. (2019a). Effect of dietary Aspergillus xylanse on nutrient digestibility and utilization, growth performance and size of internal organs in broiler chickens offered maize-soybean meal based-diets. Pakistan J. Nutr. 18(9), 852-865.
Oyeagu C.E., Mlambo V., Muchenje V. and MarumeU. (2019b). Effect of dietary supplementation of Aspergillus xylanase on broiler chickens performance. Iranian J. Appl. Anim. Sci. 9(4), 693-708.
Oyeagu C.E., Ugwuanyi C.L, Onwujiariri E., Osita C.O., Akuru E.A., Ani A.O., Idamokoro E.M. and Falowo A.B. (2019c). Blood bio-maker, growth traits, carcass characteristics and income over feed cost of broiler birds fed enzyme fortified dried brewer’s grain. Pakistan J. Nutr. 18(9), 834-844.
Panda A.K., Rama Rao S.V., Raju M.V.L.N. and Shyam Sunder G. (2009). Effect of butyric acid on performance, gastrointestinal tract health and carcass characteristics in broiler chickens. Asian-Australasian J. Anim. Sci. 22(7), 1026-1031.
Pintore G., Usai M., Bradesi P., Juliano C., Boatto G., Tomi F., Chessa M., Cerri R. and Casanova J. (2002). Chemical composition and antimicrobial activity of Rosmarinus officinalis oils from Sardinia and Corsica. Flavour Fragr. J. 17, 15-19.
Qaisrani S.N., Van Krimpen M.M., Kwakkel R.P., Verstegen M.W.A. and Hendriks W.H. (2015). Diet structure, butyric acid, and fermentable carbohydrates influence growth performance, gut morphology, and cecal fermentation characteristics in broilers. Poult. Sci. 94, 2152-2164.
Raza M., Biswas A., Mir N.A. and Mandel A.B. (2019). Butyric acid as a promising alternative to antibiotic growth promoter in broiler chicken production. J. Agric. Sci. 157(1),1-8.
Rostami H., Seidavi A., Dadashbeiki M., Asadpour Y. and Sımoes J. (2015). Effects of different dietary Rosmarinus officinalis powder and vitamin E levels on the performance and gut gross morphometry of broiler chickens. Brazilian J. Poult. Sci. 10, 23-30.
Rui X.L., Si Q.L., Zhihui Z., Li S.Q. and L.A. (2020). Sodium butyrate as an effective feed additive to improve growth performance and gastrointestinal development in broilers. Vet. Med. Sci. 6(3), 1-9.
Saeed M., Alagawany M., Fazlani S.A., Kalhoro S.A., Naveed M., Ali N., Arain M.A. and Chao S. (2019). Health promoting and pharmaceutical potential of ferulic acid for the poultry industry. World's Poult. Sci. J. 75, 83-92.
Sheikh A., Tufail B., Gulam A.B., Masood S.M. and Manzoor R. (2010). Effect of dietary supplementation of organic acids on performance, intestinal histomorphology, and serum biochemistry of broiler chicken. Vet. Med Int. 2010, 1-7.
SAS Institute. (2010). SAS®/STAT Software, Release 9.4. SAS Institute, Inc., Cary, NC. USA.
Shaaban S.E., Mahmoud A., Hamada A.M.E., Mohamed A.F. and Mayada R.F. (2020). Effect of sodium butyrate on intestinal health of poultry – a review. Ann. Anim. Sci. 20(1), 29-41.
Sharifi S.D., Khorsandi S.H., Khadem A.A., Salehi A. and Moslehi H. (2013). The effect of four medicinal plants on the performance, blood biochemical traits and ileal microflora of broiler chicks. Vet. Arhiv. 83, 69-80.
Sikandar A., Zaneb H., Younus M., Masood S., Aslam A., Khattak F., Ashraf S., Yousaf M.S. and Rehman H. (2017). Effect of sodium butyrate on performance, immune status, microarchitecture of small intestinal mucosa and lymphoid organs in broiler chickens. Asian-Australasian J. Anim. Sci. 30, 690-699.
Sleman S.M., BeskiRobert A. and SwickPaul A.I. (2015). Specialized protein products in broiler chicken nutrition: A review. Anim. Nutr. 1, 45-53.
Steel R.G.D. and Torrie J.H. (1980). Principles and Procedures of statistics. A Biometric Approach. McGraw-Hill Publishers, New York.
Thirumeignanam D., Swain R.K., Mohanty S.P. and Pati P.K. (2006). Effects of dietary supplementation of organic acids on performance of broiler chicken. Indian J. Anim. Nutr. 23(1), 34-40.
Van Immerseel F., Fievez V., de Buck J., Pasmans F., Martel A., Haesebrouck F. and Ducatelle R. (2004). Microencapsulated short-chain fatty acids in feed modify colonization and invasion early after infection with Salmonella enteritidis in young chickens. Poult. Sci. 83, 69-74.
Veselin R., Milos L., Zdenka S., Simeon R., Vladimir D., Maja P. and Aleksandar S. (2018). The effect of using rosemary (Rosmarinus officinalis) in broiler nutrition on production parameters, slaughter characteristics, and gut microbiological population. Turkish J. Vet. Anim. Sci.42, 658-664.
Wu Y., Zhou Y., Lu C., Ahmad H., Zhang H. and He J. (2016). Influence of butyrate loaded clinoptilolite dietary supplementation on growth performance, development of intestine and antioxidant capacity in broiler chickens. PLoS One. 11(4), e0154410.
Wyatt C.L., Parr T. and Bedford M. (2008). Mechanisms of action for supplemental NSP and phytase enzymes in poultry diets. Pp. 1-11 in Proc. 35th Poult. Proc. Carolina Feed Indust. Association, North Carolina,USA.
Yakhkeshi S., Rahimi S. and Hemati Matin H.R. (2012). Effects of yarrow (Achillea millefolium), antibiotic and probiotic on performance, immune response, serum lipids and microbial population of broilers. J. Agric. Sci. Technol. 14, 799-810.
Yang Z. and Liao S.F. (2019). Physiological effects of dietary amino acids on gut health and functions of Swine. Front. Vet. Sci. 6, 169-175.
Yu D.J., Na J.C., Kim S.H., Kim J.H., Kang G.H., Kim H.K., Seo O.S. and Lee J.C. (2008). Effects of dietary selenium sources on the growth performance and selenium retention of meat in broiler chickens. Proc. 13th World’s Poult. Congr. Brisbane, Queensland, Australia.
Zhang W.H., Jiang Y., Zhu Q.F., Gao F., Dai S.F., Chen J. and Zhou G.H. (2011). Sodium butyrate maintains growth performance by regulating the immune response in broiler chickens. British Poult. Sci. 52, 292-300.
Zhang A.W., Lee B.D., Lee S.K., Lee K.W., An G.H., Song K.B. and Lee C.H. (2005). Effects of yeast (Saccharomyces cerevisae) cell components on growth performance, meat quality and ileal mucosal development of broiler chicks. Poult. Sci. 84, 1015-1021.