Phenotypes, Performance, and Insulin Gene (INS) Single Nucleotide Polymorphism (SNP) C1549T Genotyping of Indonesian Meat-Type Chicken Breed

Subject Areas : Camel

D.N. Arini ¹ , M.D. Pratama ² , G.I. Firmansyah ³ , I.W.S. Mahardhika ⁴ , A.B.I. Perdamaian ⁵ , B.S. Daryono ⁶

1 - Gama Ayam Research Team, Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55281 DI Yogyakarta, Indonesia
2 - Gama Ayam Research Team, Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55281 DI Yogyakarta, Indonesia
3 - Gama Ayam Research Team, Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55281 DI Yogyakarta, Indonesia
4 - Gama Ayam Research Team, Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55281 DI Yogyakarta, Indonesia
5 - Gama Ayam Research Team, Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55281 DI Yogyakarta, Indonesia
6 - Gama Ayam Research Team, Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55281 DI Yogyakarta, Indonesia

Received: 2021-04-25 Accepted : 2021-09-16 Published : 2022-06-01

Keywords: Genotyping, indigenous breed, insulin gene, phenotypes, selective breeding,

Abstract :

Pelung chicken, as one of the Indonesian indigenous chicken breeds, is known for its distinct characteristics. Pelung chicken has been an object of selective breeding programs due to its slow-growing performance, particularly in live weight gain. Through selective breeding, the backcross and its reciprocal generations have been produced. This study was aimed to identify the phenotypes, performance in live weight gain, and insulin gene (INS) single-nucleotide polymorphism (SNP) C1549T genotyping. The phenotypes consisted of morphometrics and morphological traits. The tetra-primeramplificationrefractory mutation system polymerase chain reaction (T-ARMS-PCR) method was used to detect the SNP C1549T of the INS gene. The live weight gain of reciprocal backcross (RBC1) outperformed the first backcross (BC1) chickens. Morphometrics and morphological traits of BC1 and RBC1 indicated a directional selection effect towards pelung chicken. The transition mutation of SNP C1549T was only detected in RBC1 chickens as CT genotype (44.44%) and TT genotype (55.56%). The presence of SNP C1549T might have a significant association with the live weight gain of RBC1. T-ARMS-PCR method is suitable for the rapid detection of single nucleotide polymorphisms. Additional studies are required to confirm the association of INS gene polymorphism and live weight gain with a larger population size.

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