Genotypic and Allelic Frequencies of IGF1 and IGF2 Genes in Broilers Analysed by Using PCR‐RFLP Techniques

Subject Areas : Camel

T. Shah ¹ , S. Deshpande ² , U. Dasgupta ³ , K.M. Singh ⁴ , C.G. Joshi ⁵

1 - Department of Animal Biotechnology, College of Veterinary Science and A.H. Anand Agricultural University, Anand, 388001, Gujarat, India
2 - Marshall Breeders Pvt Limited, C and M House, N.D. Patel R.d. Nasik, 422001, Maharashtra, India
3 - Marshall Breeders Pvt Limited, C and M House, N.D. Patel R.d. Nasik, 422001, Maharashtra, India
4 - Department of Genetics, ARIBAS, New V.V. Nagar, Anand, India
5 - Department of Animal Biotechnology, College of Veterinary Science and A.H. Anand Agricultural University, Anand, 388001, Gujarat, India

Received: 2011-09-08 Accepted : 2011-10-30 Published : 2012-12-01

Keywords: PCR-RFLP, genotype, <i>IFG1</i>, <i>IGF2</i>,

Abstract :

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test was performed to investigate the allele frequencies of the insulin-like growth factor (IGF1 and IGF2 genes) in 90 broilerchickens. A 793 bp fragment of IGF1 gene and 1146 bp fragment of IGF2 gene were amplified and digested with HinfI and Hsp92II restriction enzymes,respectively. Two types of alleles of A and B and three types of genotypes of AA, AB and BB were observed in both genes. The results indicated predominant B-allele (0.85) was higher for IGF1 butA-allele (0.5611) IGF2 in the broiler population.

References:

Amills M., Jime´nez N., Villalba D., Tor M., Molina E., Cubilo D., Marcos C., Francesch A., Sa`nchez A. and Estany J. (2003). Identification of three single nucleotide polymorphisms in the chicken insulin-like growth factor 1 and 2 genes and associations with growth and feeding traits. Poult.Sci. 82, 1485-1493.
Beccavin C., Chevalier B., Cogburn L.A., Simon J. and Duclos M.J. (2001). Insulin-like growth factors and body growth in chickens divergently selected for high or low        growth rate. J. Endocrinol. 168, 297-306.
Darling D.C. and Brickell P.M. (1996). Nucleotide sequence and genomic structure of the chicken insulin-like growth factor-II (IGF-II) coding region. Gen. Comp. Endocrinol. 102, 283-287.
DeChiara T.M., Robertson E.J. and Efstratiadis A. (1991). Parental imprinting of the mouse insulin-like growth factor II gene. Cell. 64, 849-859.
Gouda E.M., Essawy G.S. (2010). Polymorphism of insulin-like growth factor Igene among chicken breeds in Egypt. Z. Naturforsch. C. 65, 284-288.
Hoeflich A., Mohan S., Foll J., Wanke R., Froehlich T., Arnold G.J., Lahm H., Kolb H.J. and Wolf E. (1999).Overexpression of insulin-like growth factor-binding protein-2 in transgenic mice reduces postnatal BW gain. Endocrinology. 140, 5488-5496.
Huybrechts L.M., Decuypere E., Buyse J., Kuhn E.R. and Tixier-Boichard M. (1992). Effect of recombinant human insulinlike growth factor-I on weight gain, fat content and hormonal parameters in broiler chickens. Poult. Sci. 71, 181-187.
John S.W.M., Weitzner G.,Rozen R. and Scriver C.R. (1991). A rapid procedure for extracting genomic DNA from leukocytes. NucleicAcids Res. 19(2), 408-413.
Kajimoto Y. and Rotwein P. (1991). Structure of the chicken insulin-like growth factor I gene reveals conserved promoter elements. J. Biol. Chem. 266, 9724-9731.
Killian J.K., Nolan C.M., Stewart N., Munday B.L., Andersen N.A., Nicol S. and Jirtle R.L. (2001). Monotreme IGF2 expression and ancestral origin of genomic imprinting. J. Exp.Zool. 291, 205-212.
Klein S., Morrice D.R., Sang H., Crittenden L.B. and Burt D.W. (1996). Genetic and physical mapping of the chicken IGF1 gene to chromosome 1 and conservation of synteny with other vertebrate genomes. J. Hered. 87, 10-14.
Leng L., Wang S., Li Z., Wang Q. and Li H. (2009). A polymorphism in the 3'-flanking region of insulin-like growth factor binding protein 2 gene associated with abdominal fat in chickens. Poult. Sci. 88, 938-942.
Li Z.H., Li H., Zhang H., Wang S.Z., Wang Q.G. and Wang Y.X. (2006). Identification of a single nucleotide polymorphism of the insulin-like growth factor binding protein 2 gene and its association with growth and body composition traits in the chicken. J. Anim. Sci. 84, 2902-2906.
McMurtry J.P. (1998). Nutritional and developmental roles of insulin-like growth factors in poultry. J. Nutr. 128, 302-305.
Nagao K., Oki M., Tsukada A. and Kita K. (2011). Alleviation of body weight loss by dietary methionine is independent of insulin-like growth factor I in protein-starved young chickens. Anim. Sci. J. 82, 560-564.
Nagaraja S.C., Aggrey S.E., Yao J., Zadworny D., Fairfull R.W. and Kuhnlein U. (2000). Trait association of a genetic marker near the IGF-I gene in egg-laying chickens. J. Hered. 91, 150-156.
Nolan C.M., Killian J.K., Petitte J.N. and Jirtle R.L. (2001). Imprint status ofM6P /IGF2R and IGF2 in chickens. Dev. Genes. Evol. 211, 179-183.
O′Neill M.J., Ingram R.S., Vrana P.B. and Tilghman S.M. (2000). Allelic expression of IGF2 in marsupials and birds. Dev.Genes. Evol. 210, 18-20.
Spencer G.S., Decuypere E., Buyse J., Hodgkinson S.C., Bass J.J. and Zeman M. (1995). Passive immunization of insulin like growth factor (IGF)-1 and of IGF1 and IGF2 in chickens. Comp. Biochem. Physiol. C. Pharmacol. Toxicol. Endocrinol. 110, 29-33.
Tanaka M., Hayashida Y., Sakaguchi K., Ohkubo T., Wakita M., Hoshino S. and Nakashima K. (1996). Growth hormone-independent expression of insulin-like growth factor I messenger ribonucleic acid in extra hepatic tissues of the chicken. Endocrinology. 137, 30-34.
Tixier-Boichard M., Huybrechts L.M., Decuypere E., Kuhn E.R., Monvoisin J.L., Coquerelle G., Charrier J. and Simon J. (1992). Effects of insulin-like growth factor-I (IGF-I) infusion and dietary tri-iodothyronine (T3) supplementation on growth, body composition and plasma hormone levels in sex-linked dwarf mutant and normal chickens. J. Endocrinol. 133, 101-110.
Tomas F.M., Pym R.A., McMurtry J.P. and Francis G.L. (1998). Insulin-like growth factor (IGF)-I but not IGF-II promotes lean growth and feed efficiency in broiler chickens. Gen. Comp. Endocrinol. 110, 262-275.
Wu G., Siegel B.P., Gilbert R.E., Yang N. and Wong A.E. (2011). Expression profiles of somatotropic axis genes in lines of chickens divergently selected for 56-day body weight. Anim. Biotech. 22,100-110.
Yan B.X., Li N., Deng X.M., Hu X.X., Liu Z.L., Zhao X.B., Lian Z.X. and Wu C.X. (2002). Single nucleotide polymorphism analysis in chicken insulin-like growth factor-II gene and its associations with growth and carcass traits. Yi. Chuan Xue Bao Acta. genetica Sinica. 29, 30-33.
Yokomine T., Kuroiwa A., Tanaka K., Tsudzuki M., Matsuda Y. and Sasaki H. (2001). Sequence polymorphisms, allelic expression status and chromosome locations      of the chicken IGF2 and MPR1 genes. Cytogenet. Cell Genet. 93, 109-113.
Zhou M., Ma Z. and Sly W.S. (1995). Cloning and expression of the cDNA of chicken cation-independent mannose-6-phosphate receptor. Proc. Natl. Acad. Sci. 92, 9762-9766.