Effect of Aqueous-Alcoholic Extract of Hibiscus sabdariffa Calyx and Leaf Calyx and Leaf on Performance, Egg Quality, Immune System and Antioxidant Balance of Laying Hens
Subject Areas : Camelش. ثابت سروستانی 1 , س.م. حسینی 2 * , س.ه. فرهنگ فر 3
1 - Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran
3 - Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran
Keywords: yolk cholesterol, Malondialdehyde, egg shell, <i>H. sabdariffa</i>,
Abstract :
This experiment was conducted to obtain information on how aqueous-alcoholic extractof H. sabdariffa plant can affect laying hen performance, egg quality, immune system and antioxidant balance during thermo-neutrals conditions. Two hundred hens (23-wk-old) were divided into 5 experimental treatments with 4 replicates each, 10 white-egg Hy-Line (W36) hens per cage. Treatment 1 (control) birds were fed with a corn and soybean meal-based diet without extract, whereas treatments 2 and 3 received, 300 and 700 mg/kg of H. sabdariffa leaf extract (HSLE) and treatments 4 and 5, received 300 and 700 mg/kg of H. sabdariffa calyx extract (HSCE), respectively. The weekly recorded performance data included egg production, feed intake, egg mass and feed conversion ratio. The egg quality parameters were evaluated at each 28 days of the experimental periods. Two hens from each replicate were selected and the blood was gathered to determine the immune system and plasma malondialdehyde (MDA). Also, two egg yolks from each replicate were used to investigated yolk MDA, cholesterol and triglyceride. Comparing the results with the control showed, HSCE at 700 mg/kg promoted laying rate during peak production (p <0.05) up to 8%. However, antioxidant balance and immune function did not differ between treatment groups. Eggshell strength, eggshell thickness, eggshell weight and egg-shape index were affected by treatments (p <0.05). Hens receiving 700 mg/kg of HSLE significantly decreased yolk cholesterol (p <0.05) compared to the control group (44.74 vs. 47.67). The results suggest that the H. sabdariffa can improve laying performance, egg qualitative characteristics and reduce egg yolk cholesterol during peak production period. The beneficial effects of the H. sabdariffa should be further studied in commercial production conditions.
Al-Batshan H., Scheideler S., Black B., Garlich J. and Anderson K. (1994). Duodenal calcium uptake, femur ash, and eggshell quality decline with age and increase following molt. Poult. Sci. 73, 1590-1596.
Allan W. and Gough R. (1974). A standard haemagglutination inhibition test for Newcastle disease. (1). a comparison of macro and micro methods. Vet. Res. 95, 120-123.
AOAC. (2007). Official Methods of Analysis. 18th Ed. Association of Official Analytical Chemists, Arlington, Washington, DC., USA.
Calderon V.M. and Jensen L.S. (1990). The requirement for sulfur amino acid by laying hens as influenced by the protein concentration. Poult. Sci. 69, 934-944.
Carvajal-Zarrabal O., Waliszewski S.M., Barradas-Dermitz D.M., Orta-Flores Z., Hayward-Jones P.M., Nolasco-Hipólito C., Angulo-Guerrero O., Sánchez-Ricaño R., Infanzón R.M. and Trujillo P.R. (2005). The consumption of Hibiscus sabdariffa dried calyx ethanolic extract reduced lipid profile in rats. Plant Foods Hum. Nutr. 60, 153-159.
Cheng T.K., Coon C.N. and Hamre M.L. (1990). Effect of environmental stress on the ascorbic acid requirement of laying hens. Poult. Sci. 69, 774-780.
Cherian G., Wolfe F. and Sim J. (1996). Dietary oils with added tocopherols, effects on egg or tissue tocopherols, fatty acids, and oxidative stability. Poult. Sci. 75, 423-431.
Chuah A.M., Lee Y.C., Yamaguchi T., Takamura H., Yin L.J. and Matoba T. (2008). Effect of cooking on the antioxidant properties of coloured peppers. Food Chem. 111, 20-28.
El-Ghalid O. (2009). Exogenous estradiol, blood profile, productive and reproductive performance of female japanese quails at different stages of production. Asian J. Poult. Sci. 3, 1-8.
Fakeye T. (2008). Toxicity and immunomodulatory activity of fractions of Hibiscus sabdariffa (family Malvaceae) in animal models. African J. Tradit. Complement. Altern. Med. 5, 394-398.
Fakeye T.O., Pal A., Bawankule D. and Khanuja S. (2008). Immunomodulatory effect of extracts of Hibiscus sabdariffa (family Malvaceae) in a mouse model. Phytother. Res. 22, 664-668.
Ginter E., Kubec F., Vozar J. and Bobek P. (1979). Natural hypocholesterolemic agent, pectin plus ascorbic acid. Int. J. Vitam. Nutr. Res. 49, 406-412.
Gross W. and Siegel H. (1983). Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Dis. 27, 972-979.
Hirunpanich V., Utaipat A., Morales N.P., Bunyapraphatsara N., Sato H., Herunsalee A. and Suthisisang C. (2005). Antioxidant effects of aqueous extracts from dried calyx of Hibiscus sabdariffa (Roselle) in vitro using rat low-density lipoprotein (LDL). Biol. Pharm. Bull. 28, 481-484.
Hirunpanich V., Utaipat A., Morales N.P., Bunyapraphatsara N., Sato H., Herunsale A. and Suthisisang C. (2006). Hypocholesterolemic and antioxidant effects of aqueous extracts from the dried calyx of Hibiscus sabdariffa in hypercholesterolemic rats. J. Ethnopharmacol. 103, 252-260.
Kahraman Z. (2009). Herbal feed additive and their usage in laying hen diets. J. Poult. Res. 8, 34-41.
Keshavarz K. (1996). The effect of different levels of vitamin C and cholecalciferol with adequate or marginal levels of dietary calcium on performance and eggshell quality of laying hens. Poult. Sci. 75, 1227-1235.
Khan R., Naz S., Nikousefat Z., Selvaggi M., Laudadio V. and Tufarelli V. (2012). Effect of ascorbic acid in heat-stressed poultry. Worlds Poult. Sci. J. 68, 477-490.
Kim J.K., So H., Youn M.J., Kim H.J., Kim Y., Park C., Kim S.J., Ha Y.A., Chai K.Y., Kim S.M., Kim K.Y. and Park R. (2007). Hibiscus sabdariffa water extract inhibits the adipocyte differentiation through the PI3-K and MAPK pathway. J. Ethnopharmacol. 114, 260-267.
Kwari I., Igwebuike J., Mohammed I. and Diarra S. (2011). Growth, haematology and serum chemistry of broiler chickens fed raw or differently processed sorrel (Hibiscus sabdariffa) seed meal in a semi-arid environment. Int. J. Sci. Nat. 2, 22-27.
Lin H.H., Chen J.H. and Wang C.J. (2011). Chemopreventive properties and molecular mechanisms of the bioactive compounds in Hibiscus sabdariffa. Curr. Med. Chem. 18, 1245-1254.
Lubega A.M., Bbosa G.S., Musisi N., Erume J. and Ogwal-Okeng J. (2013). Effect of the total crude extracts of Hibiscus sabdariffa on the immune system in the Wistar albino rats. African J. Pharm. Pharmacol. 7, 1942-1949.
Luhman C.M., Miller B.G. and Beitz D.C. (1990). Research note, the effect of feeding lovastatin and colestipol on production and cholesterol content of eggs. Poult. Sci. 69, 852-855.
McReynolds J.L., Genovese K.J., He H., Swaggerty C.L., Byrd J.A., Ricke S.C., Nisbet D.J. and Kogut M.H. (2009). Alfalfa as a nutritive modulator in maintaining the innate immune response during the molting process. J. Appl. Poult. Res. 18, 410-417.
McFarlane J.M. and Curtis S.E. (1989). Multiple concurrent stressors in chicks. 3. effects on plasma corticosterone and the heterophil, lymphocyte ratio. Poult. Sci. 68, 522-527.
Miller N.J., Rice-Evans C., Davies M.J., Gopinathan V. and Milner A. (1993). A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin. Sci. 84, 407-412.
Minka N., Fayomi A. and Ayo J. (2007). Protective influence calyces of Hibiscus sabdariffa against heat stress in laying hens during the hot-dry season. Res. J. Poult. Sci. 1, 7-11.
Morton J. (1987). Roselle. Pp. 281-286 in Fruits of Warm Climates. J.F. Morton, Ed. Creative Resource Systems Inc., Miami, Florida.
Mungole A. and Chaturvedi A. (2011). Hibiscus sabdariffa a rich source of secondary metabolites. Int. J. Pharm. Sci. Rev. Res. 6, 83-87.
Ni Y., Zhu Q., Zhou Z., Grossmann R., Chen J. and Zhao R. (2007). Effect of dietary daidzein on egg production, shell quality, and gene expression of ER-α, GH-R, and IGF-IR in shell glands of laying hens. J. Agric. Food Chem. 55, 6997-7001.
North M.O. (1984). Commercial Chicken Production Manual. The Avi Publishing Company Inc., Dawsonville, Georgia.
Ochani P.C. and Mello P.D. (2009). Antioxidant and antihyperlipidemic activity of Hibiscus sabdariffa leaves and calyces extracts in rats. Indian J. Exp. Biol. 47, 276-282.
Okoko T. and Ere D. (2012). Hibiscus sabdariffa extractivities on cadmium-mediated alterations of human U937 cell viability and activation. Asian Pac. J. Trop. Med. 5, 33-36.
Olaleye M.T. (2007). Cytotoxicity and antibacterial activity of methanolic extract of Hibiscus sabdariffa. J. Med. Plants Res. 1, 9-13.
Olatunji L.A., Adebayo J.O., Oguntoye O.B., Olatunde N.O., Olatunji V.A. and Soladoye A.O. (2005). Effects of aqueous extracts of petals of red and green Hibiscus sabdariffa on plasma lipid and hematological variables in rats. Pharm. Biol. 43, 471-474.
Ologundudu A., Ologundudu A., Oluba O., Omotuyi I. and Obi F. (2010). Effect of Hibiscus sabdariffa anthocyanins on 2, 4-dinitrophenylhydrazine-induced tissue damage in rabbits. J. Toxicol. Environ. Health Sci. 2, 1-6.
Omotuyi I., Ologundudu A., Onwubiko V. and Wogu M. (2011). Hibiscus sabdariffa anthocyanins alter circulating reproductive hormones in rabbits. J. Diabetes Endocrinol. 1, 36-45.
Pandey K.B. and Rizvi S.I. (2009). Plant polyphenols as dietary antioxidants in human health and disease. Oxid. Med. Cell. Longev. 2, 270-278.
Picotto G., Massheimer V. and Boland R. (1996). Acute stimulation of intestinal cell calcium influx induced by 17β-estradiol via the cAMP messenger system. Mol. Cell. Endocrinol. 119, 129-134.
Radwan N.L., Hassan R., Qota E. and Fayek H. (2008). Effect of natural antioxidant on oxidative stability of eggs and productive and reproductive performance of laying hens. Int. J. Poult. Sci. 7, 134-150.
Renard C.M., Watrelot A.A. and Le Bourvellec C. (2017). Interactions between polyphenols and polysaccharides: mechanisms and consequences in food processing and digestion. Trend. Food Sci. Technol. 60, 43-51.
Rice-Evans C.A. and Miller N.J. (1996). Antioxidant activities of flavonoids as bioactive components of food. Biochem. Soc. Trans. 24, 790-795.
Sabet Sarvestani S., Hosseini S.M. and Farhangfar H. (2019). The effect of sour of tea (Hibiscus sabdariffa) plant extract on immune system, plasma antioxidant status, oxidant balance and blood biochemical and functional parameters of old laying hens. Iranian J. Anim. Sci. Res. 12, 22-31.
Saeed I.A., Ali L., Jabeen A., Khasawneh M., Rizvi T.A. and AshrafS.S. (2013). Estrogenic activities of ten medicinal herbs from the middle east. J. Chromatogr. Sci. 51, 33-39.
Sahin K., Orhan C., Tuzcu M., Ali S., Sahin N. and Hayirli A. (2010). Epigallocatechin-3-gallate prevents lipid peroxidation and enhances antioxidant defense system via modulating hepatic nuclear transcription factors in heat-stressed quails. Poult. Sci. 89, 2251-2258.
Sahin K., Ozercan R., Onderci M., Sahin N., Gursu M.F., Khachik F., Sarkar F.H., Munkarah A., Ali-Fehmi R. and Kmak D. (2004). Lycopene supplementation prevents the development of spontaneous smooth muscle tumors of the oviduct in Japanese quail. Nutr. Cancer. 50, 181-189.
Sahin K., Sahin N., Onderci M., Yaralioglu S. and Kucuk O. (2001). Protective role of supplemental vitamin E on lipid peroxidation, vitamins E, A and some mineral concentrations of broilers reared under heat stress. Vet. Med. 46, 140-144.
Saki A.A., Rahmati M.M., Zamani P., Zaboli K. and Matin H.R. (2010). Can vitamin C elevate laying hen performance, egg and plasma characteristics under normal environmental temperature? Italian J. Anim. Sci. 9, 313-317.
SAS Institute. (2015). SAS®/STAT Software, Release 9.4. SAS Institute, Inc., Cary, NC. USA.
Smith W.S., Green C. and Jerry M. (2003). Determination of ascorbic acid (vitamin C) in commerical tablets by iodometric titration. MS Thesis. Stevens Institute of Technology, Hoboken, USA.
Sukkhavanit P., Angkanaporn K. and Kijparkorn S. (2011). Effect of roselle (Hibiscus sabdariffa) calyx in laying hen diet on egg production performance, egg quality and TBARS value in plasma and yolk. Thai J. Vet. Med. 41, 337-344.
Sullivan A.C., Hamilton J.G., Miller O.N. and Wheatley V.R. (1972). Inhibition of lipogenesis in rat liver by (−)-hydroxycitrate. Arch. Biochem. Biophys. 150, 183-190.
Swain T. (1965). Analytical methods for flavonoids. Pp. 543-544 in The Chemistry and Biochemistry of Plant Pigments. T.W. Goodwin, Ed. Academic Press, London, United Kingdom.
Tseng T.H., Kao E.S., Chu C.Y., Chou F.P., Wu H.W.L. and Wang C.J. (1997). Protective effects of dried flower extracts of Hibiscus sabdariffa against oxidative stress in rat primary hepatocytes. Food Chem. Toxicol. 35, 1159-1164.
Turkmen N., Sari F. and Velioglu Y.S. (2005). The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Food Chem. 93, 713-718.
Vanzo A., Terdoslavich M., Brandoni A., Torres A.M., Vrhovsek U. and Passamonti S. (2008). Uptake of grape anthocyanins into the rat kidney and the involvement of bilitranslocase. Mol. Nutr. FoodRes. 52, 1106-1116.
Wang H., Cao G. and Prior R.L. (1997). Oxygen radical absorbing capacity of anthocyanins. J. Agric. Food Chem. 45, 302-309.
Wang J., Cao X., Jiang H., Qi Y., Chin K.L. and Yue Y. (2014). Antioxidant activity of leaf extracts from different Hibiscus sabdariffa accessions and simultaneous determination five major antioxidant compounds by LC-Q-TOF-MS. Molecules. 19, 21226-21238.
Wang J., Yue H., Wu S., Zhang H. and Qi G. (2017). Nutritional modulation of health, egg quality and environmental pollution of the layers. Anim. Nutr. 3, 91-96.
Wong P.K., Yusof S., Ghazali H. and Che Man Y. (2002). Physico-chemical characteristics of roselle (Hibiscus sabdariffa). Nutr. Food Sci. 32, 68-73.
Yoshioka T., Kawada K., Shimada T. and Mori M. (1979). Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am. J. Obstet. Gynecol. 135, 372-376.
Zhao R.Q., Zhou Y.C., Ni Y.D., Lu L.Z., Tao Z.R., Chen W.H. and Chen J. (2005). Effect of daidzein on egg-laying performance in Shaoxing duck breeders during different stages of the egg production cycle. British Poult. Sci. 46, 175-181.
Zhou E., Pan X. and Tian X. (2009). Application study of xylo-oligosaccharide in layer production. Mod. Appl. Sci. 3, 103-107.