Effects of Centrifugation and Different Levels of Soybean Lecithin-Based Extender on Post-Thaw Sperm Quality of Ghezel Ram Semen
Subject Areas : Camel
م.
شمسالهی
1
(Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
ح.
دقیقکیا
2
(Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
غ.
مقدم
3
(Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
ا.
تقیزاده
4
(Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
Keywords: sperm, freeze-thawing, centrifugation, soybean lecithin,
Abstract :
The objective of this study was to specify the effects of centrifugation and different surface of soybean lecithin-based extender on post-thaw Sperm quality of Ghezel ram semen. Semen samples were gathered from 5 mature Ghezel ram two/week for 3 weeks. After initial evaluation, the approved semen samples were pooled together and split into equal parts in Falcon tubes. Three samples were combined with Tris buffer at 30 ˚C and then centrifuged to remove the seminal plasma. After centrifugation and removal of the supernatant, samples were diluted with Tris-citrate-fructose extender + different concentrations of soybean lecithin (1%, 1.5% and 2% weight/volume) with 7% glycerol; and residual one samples, which were not centrifuged, were diluted with the (1.5% weight/volume) soybean lecithin. Samples then cooled to 5 ˚C and frozen in 0.25 mL French straws and straws were thawed in a 37 ˚C water bath. The results of different concentrations of soybean lecithin showed that total motility (TM) and motion parameters of average path velocity (VAP), straight-line velocity (VSL), curvilinear velocity (VCL) and lateral head displacement (ALH) and and the percentage of spermatozoa with intact acrosomes were significantly higher in soybean lecithin (SL) 1.5% compared to other semen extenders (P<0.05). Total motility and sperm-motion parameters (VAP, VSL, VCL and ALH), in SL1.5% without centrifugation were higher than the groups with 1%, 1.5% or 2% and with centrifugation (P<0.05). Generally, the results suggest that SL1.5% extender was better (with or without centrifugation) than other extenders in most in vitro evaluated sperm parameters.
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