Evaluation of Nano and Microcapsules of Silymarin in Simulated Gastrointestinal Conditions for Animal Target Delivery
Subject Areas : Camelس. یوسفدوست 1 , ف. صمدی 2 * , س.م. جعفری 3 , س.س. رمضانپور 4 , ف. گنجی 5 , س. حسنی 6
1 - Department of Animal and Poultry Physiology, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
2 - Department of Animal and Poultry Physiology, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
3 - Department of Food Materials and Process Design Engineering, Faculty of Food Science and Technology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
4 - Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
5 - Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran
6 - Department of Animal and Poultry Physiology, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
Keywords: nano/microcapsule, simulated gastrointestinal conditions, ultrasonication, <i>Silybum marianum</i>,
Abstract :
The main goal of this research was to compare the in vitro release rates of nano- and microcapsules of Silybum marianum extract (SME) in animal simulated gastric and intestinal medium conditions. The extract was encapsulated within sodium alginate carriers using emulsification/internal gelation method. Particle size, zeta potential, polydispersity index (PDI) and morphology of nanocapsules were analysed via dynamic light scattering (DLS) and transmission electron microscopy (TEM). In addition, the effect of ultrasonication on nanocapsule properties and the release profiles of SME-loaded nano/microcapsules were evaluated. Results showed that ultrasonication reduced the size of capsules from 657.5 nm to 169.1 nm which resulted in uniform particles with a low PDI. Encapsulation efficiency for nanocapsules was 61%. Alginate nano/microcapsules protected polyphenols in simulated gastric medium as observed by 10% and 12% release, respectively. Nanocapsules released their contents higher and faster than microcapsules in simulated intestinal fluid (P<0.05). In conclusion, alginate nanocapsules containing SME were made successfully with a release rate of over 90% of extract within simulated intestinal medium which can be used for animal target delivery purposes.
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