Effects of Concentrate Source (Cottonseed vs. Barley)on Milk Performance and Fatty Acids Profile of Holstein-Friesian Dairy Cows

Subject Areas : Camel

A.I. Roca-Fernandez ¹ (Centro de Investigaciones Agrarias de Mabegondo, INGACAL, Apdo, 10, 15080, A Coruña, Spain)
A. Gonzalez-Rodriguez ² (Centro de Investigaciones Agrarias de Mabegondo, INGACAL, Apdo, 10, 15080, A Coruña, Spain)
O.P. Vazquez-Yanez ³ (Fondo Gallego de Garantía Agraria, Apdo, 15781, Santiago de Compostela, Spain)
J.A. Fernandez-Casado ⁴ (Laboratorio Agrario y Fitopatológico de Galicia, Apdo, 365, 15640, A Coruña, Spain)

Received: 2011-01-19 Accepted : 2011-03-27 Published : 2011-12-01

Keywords: lipid feed supplements, cottonseed, Linoleic acid, milk fat content, total mixed ration,

Abstract :

Milk performance (protein and fat content) and milk fatty acids (FA) profile of dairy cows under supplementation with two sources of concentrate, oilseeds (C, cottonseed) at two levels, low (5kg DM/cow/day) and high (7 kg DM/cow/day), and cereal grains (B, barley) at high rate (7 kg DM/cow/day), were studied in three herds (n=36) of spring calving Holstein-Friesian dairy cows (200 days in milk). Animals were randomly assigned to one of three indoors (n=12) silage feeding regimes (C5, C7 and B7), using a TMR (total mixed ration) basal diet, containing 70-80% silage (grass: maize, 36: 64) and 30-20% concentrate. Daily milk yield (MY) was higher (P<0.001) at high level of supplementation (B7, 18.1andC7, 17.9 kg/cow/day, respectively) compared to low level (C5, 15.7 kg/cow/day), and dairy cows at the highest level of concentrate showed the highest (P<0.05) body weight (B7, 605 and C7, 598, respectively). Milk protein content was lower (P<0.05) in the high level of cottonseed (C7, 30.7 g/kg DM) than in the barley treatment (B7, 32.7 g/kg DM). There were no differences among treatments in milk fat and milk urea content.Weekly milk FA profile of cow milk was determined using gas chromatography-mass spectrometry,during seventy days in autumn, and no differences were found among treatments in short, medium and long chain FA. Despite this, higher (P<0.05) contents of polyunsaturated fatty acids (PUFA) and linoleic acid were found in the C7 treatment compared to the C5 treatment (2.48 and 2.22 vs. 2.16 and 1.92 g/100 g of FA methyl esters, respectively). Including the high levels of cottonseed and barley as a concentrate source for feeding dairy cattle revealed similar MY and milk fat content. However, the high level of cottonseed in the diet of dairy cows showed decreased milk protein content and increased linoleic acid and PUFA levels.

References:

AbuGhazaleh A.A. and Holmes L.D. (2007). Diet supplementationwith fish oil and sunflower oil to increase conjugated linoleic acidlevels in milk fat of partially grazing dairy cows. J. Dairy Sci.90,2897-2904.
Bauman D.E. and Griinari J.M. (2001). Regulation and nutritional manipulation of milk fat: low-fat milk syndrome. Livest. Prod. Sci. 70, 15-29.
Bauman D.E. and Griinari J.M. (2003). Nutritional regulation ofmilk fat synthesis. Annu. Rev. Nutr. 23, 203-227.
Baumgard L.H., Weber W.J., Kazmer G.W., Zinn S.A., Hansen L.B., Chester-Jones H. and Crooker B.A. (2002). Trans-10, cis-12 conjugated linoleic acids decreases lipogenic rates and expression of genes involved in milk lipid synthesis in dairy cows.J. Dairy Sci. 85, 2155-2163.
Bligh E.G. and Dyer W.J. (1959). A rapid method of total lipid extraction and purification. Canadian  J. Biochem. Physiol. 37, 911-917.
Bu D.P., Wang J.Q., Dhiman T.R. and Liu S.J. (2007). Effectiveness of oils rich in linoleic and linolenic acids to enhance conjugated linoleic acid in milk from dairy cows. J. Dairy Sci. 90, 998-1007.
BurgosS., Dai M. and Cant J. (2010). Nutrient availability and lactogenic hormones regulate mammary protein synthesis through the mammalian target of rapamycin signaling pathway. J. Dairy Sci. 93, 153-161.
Cant J.P., DePeters E.J. and Baldwin R.L. (1993a). Mammary uptake of energy metabolites in dairy cows fed fat and its relationship to milk protein depression. J. Dairy Sci. 76(8), 2254-2265.
Cant J.P., DePeters E.J. and BaldwinR.L. (1993b). Mammary amino acid utilization in dairy cows fed fat and its relationship to milk protein depression. J. Dairy Sci. 76(3), 762-774.
Castro-García P. (1994). Espectroscopía de reflectancia en el infrarrojo próximo (NIRS) y evaluación nutritiva de pastos. Tesis doctorales en microfichas 408. Servicio de Publicaciones de la Universidad de Santiago, Galicia, España. Pp. 121.
Chilliard Y., Ferlay A. and Doreau M. (2001). Effect of different typesof forages, animal fat or marine oils in cow’s diet on milk fatsecretion and composition, especially conjugated linoleic acids(CLA) and polyunsaturated fatty acids. Livest. Prod. Sci.70,31-48.
Clapperton J.L. and Banks W. (1985). Factors affecting the yield of milk and its constituents, particularly fatty acids, when dairy cows consume diets containing added fat. J. Sci. Food Agric. 36, 1205-1211.
DePeters E.J. and Cant J.P. (1992). Nutritional factors influencing the nitrogen composition of bovine milk: A review. J. Dairy Sci. 75(8), 2043-2070.
Fagan P., Wijesundera C. and Watkins P. (2004). Determination of mono- and di-acylglycerols in milk lipids. J. Chromatogr. A 1054(1,2), 251-259.
Hansen H.O. and Knudsen J. (1987). Effect of exogenous long chain fatty acids on individual fatty acid synthesis by dispersed ruminant mammary gland cells. J. Dairy Sci. 70, 1350-1354.
Harfoot C.G. and Hazlewood G.P. (1997). Lipid metabolism in therumen. Pages 382–426 in The Rumen Microbial Ecosystem. P.N. Hobson and C.S. Stewart. (1997). Chapman and Hall, London, UK.
Harvatine K.J. and Allen M.S. (2006). Fat supplements affect fractional rates of ruminal fatty acid biohydrogenation and passage in dairy cows. J. Nutr. 136, 677-685.
Kalscheur K.F., Teter B.B., Piperova L.S. and Erdman R.A.(1997). Effect of dietary forage concentration and buffer addition onduodenal flow of trans-C18:1 fatty acids and milk fat production indairy cows.J. Dairy Sci. 80, 2104-2114.
Lock A.L. and Garnsworthy P.C. (2002). Independent effects of dietary linoleic and linolenic fatty acids on the conjugated linoleic acid content of cows’ milk. Anim. Sci. 74, 163-176.
Loor J.J., Ueda K., Ferlay A., Chilliard Y. and Doreau M. (2004). Biohydrogenation, duodenal flow, and intestinal digestibility of trans fatty acids and conjugated linoleic acids in response to dietary forage:concentrate ratio and linseed oil in dairy cows. J. Dairy Sci. 87, 2472-2485.
Loor J.J., Ferlay A., Ollier A., Doreau M. and Chilliard Y. (2005).Relationship among trans and conjugated fatty acids and bovinemilk fat yield due to dietary concentrate and linseed oil. J. DairySci.88, 726-740.
Mele M., Buccioni A., Petacchi F., Serra A., Banni S., Antongiovanni M. and Sechiari P. (2006). Effect of forage/concentrate ratio and soybean oil supplementation on milk yield, and composition from Sarda ewes. Anim. Res. 55, 273-285.
Piperova L.S., Teter B.B., Bruckental I., Sampugna J., Mills S.E., Yurawecz M.P., Fritsche J., Ku Y. and Erdman R.A. (2000). Mammary lipogenic enzyme activity, trans fatty acids and conjugated linoleic acids arc altered in lactating dairy cows fed a milk fat-depressing diet. J. Nutr. 130, 2568-2574.
Piperova L.S., Sampugna J., Teter B.B., Kalscheur K.F., Yurawecz M.P., Ku Y., Morehouse K.M. and Erdman R.A. (2002). Duodenal and milk trans octadecenoic acid and conjugated linoleic acid (CLA) isomers indicate that postabsorptive synthesis is the predominant source of cis-9- containing CLA in lactating dairy cows. J. Nutr. 132, 1235-1241.
Precht D. and Molketin J. (2000). Frequency of conjugated linoleic acid and trans fatty acid contents in European bovine milk fats. Milchwissenschaft 55, 687-691.
Pulina G., Nudda A., Battacone G. and Cannas A. (2006). Effects of nutrition on the contents of fat, Protein, somatic cells, aromatic compounds, and undesirable substances in sheep milk. Anim. Feed Sci. Technol. 131, 255-291.
Roca-Fernández A.I., González-Rodríguez A., Vázquez-Yáñez O.P. and Fernández-Casado J.A. (2010). High reliance on grass for an improved milk fatty acids composition.J. Dairy Sci. 93 (E-Suppl. 1), 757.
Roy A., Ferlay A., Shingfield K.J. and Chilliard Y. (2006). Examination of the persistency of milk fatty acid composition responses to plant oils in cows given different basal diets, with particular emphasis on trans-C18:1 fatty acids and isomers of conjugated linoleic acid. Anim. Sci. 82, 479-492.
Ruppert L.D., Drackley J.K., Bremmer D.R. and ClarkJ.H. (2003). Effects of tallow in diets based on corn silage or alfalfa silage on digestion and nutrient use by lactating dairy cows. J. Dairy Sci. 86, 593-609.
SAS Institute. (2005). SAS User's Guide: Statistics, SAS Institute Inc., Cary, NC, USA. 49 Pp.
Shingfield K.J., Reynolds C.K., Lupoli B., Toivonen V., Yurawecz M.P., Delmonte P., Griinari J.M., Grandison A.S. and Beever D.E. (2005). Effect of forage type and proportion of concentrate in the diet on milk fatty acid composition in cows given sunflower oil and fish oil. Anim. Sci. 80, 225-238.
Shingfield K.J., Reynolds C.K., Hervás G., Griinari J.M., Grandison A.S. and Beever D.E. (2006). Examination of the persistencyof milk fatty acid composition responses to fish oil and sunflower oil in the diet of dairy cows. J. Dairy Sci. 89, 714-732.
Shingfield K.J. and Griinari J.M. (2007). Role of biohydrogenation intermediates in milk fat depression. Eur. J. Lipid Sci. Tech. 109, 799-816.
Wildman E.E., Jones G.M., Wagner P.E., Boman R.L., Troutt H.F.Jr. and Lesch T.N. (1982). A dairy cow body condition scoring system and its relationship to selected production characteristics. J. Dairy Sci. 65, 495-501.