Association of IGF-I Gene Polymorphisms with Carcass Traits in Iranian Mehraban Sheep Using SSCP Analysis

Subject Areas : Camel

S. Behzadi ¹ ( Department of Animal Science, Faculty of Agricultureand Natural Resources, University of Tehran, Karaj, Iran )
S.R. Miraei-Ashtiani ² ( Department of Animal Science, Faculty of Agricultureand Natural Resources, University of Tehran, Karaj, Iran )
M. Sadeghi ³ ( Department of Animal Science, Faculty of Agricultureand Natural Resources, University of Tehran, Karaj, Iran )
P. Zamani ⁴ ( Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran )
R. Abdoli ⁵ ( Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran )

Received: 2014-01-08 Accepted : 2014-04-30 Published : 2015-03-01

Keywords: carcass traits, single nucleotide polymorphism, insulin-like growth factor I, Mehraban sheep, single-strand conformational polymorphism,

Abstract :

Molecular genetics selection on individual genes is a promising method to genetically improve economically important traits in livestock. The insulin like growth factor-I (IGF-I) gene may play important roles in growth of multiple tissues, including muscle cells, cartilage and bone. The objectives of the present study were the estimate the haplotype frequencies of the IGF-I gene polymorphisms in Iranian Mehraban sheep and to determine associations between polymorphisms and carcass traits in 439 individuals. Polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) and DNA sequencing methods were employed in screening for genetic variation. Two single nucleotide polymorphisms (C114G and G116A) and two amino acid exchange (S5T and G6S) withthree patterns were found in IGF-I gene fragment. Associations of IGF-I patterns with blood cholesterol and blood triglycerides were significant (P<0.05) and tended to be significant (P<0.1) for dorsal fat thick and carcass weight. The other studied traits were not significantly affected by different patterns of IGF-I. This finding indicates that IGF-I polymorphisms may be used as DNA markers for selection in the breeding process of Mehraban sheep.

References:

Adam C.L., Gadd T.S., Findlay P.A. and Wathes D.C. (2000). IGF-I stimulation of luteinizing hormone secretion, IGF binding proteins (IGFBPs) and expression of mRNAs for IGFs, IGF receptors and IGFBPs in the bovine pituitary gland. J. Endocrinol. 166, 247-254.
Akers R.M. (2006). Major advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows. J. Dairy Sci. 89, 1222-1234.
Anderson P.T., Bergen W.G., Merkel R.A., Enright W.J., Zinn S.A., Refsal K.R. and Hawkins D.R. (1988). The relationship between composition of gain and circulating hormones in growing beef bulls fed three dietary crude protein levels. J. Anim. Sci. 66, 3059-3067.
Andrade P.C., Grossi D.A., Paz C.C., Alencar M.M., Regitano L.C. and Munari D.P. (2008). Association of an insulin-like growth factor 1 gene microsatellite with phenotypic variation and estimated breeding values of growth traits in Canchim cattle. Anim. Genet. 39, 480-485.
Barash H., Aharoni Y., Brosh A. and Holzer Z. (1998). Effect of low energy diets followed by a compensatory diet on bodyweight gain and plasma hormone concentrations in bull calves. J. Dairy Sci. 81, 250-254.
Bryne P.F. and McMullen M.D. (1996). Defining genes for agricultural traits: QTL analyses and the candidate gene approach. Probe. 7, 24-27.
Burgos S.A. and Cant J.P. (2010). IGF-I stimulates protein synthesis by enhanced signaling through mTORC1 in bovine mammary epithelial cells. Domest. Anim. Endocrinol. 38, 211-221.
Casas-Carrillo E., Prill-Adams A., Price S.G., Clutter A.C. and Kirkpatrick B.W. (1997). Relation of growth hormone and insulin-like growth factor-I genotypes with carcass traits in swine. Anim. Genet. 28, 88-93.
Curi R.A., Oliveira H.N., Silveira A.C. and Lopes C.R. (2004). Effects of polymorphic microsatellites in the regulatory region of IGF-I and GHR on growth and carcass traits in beef cattle.Anim. Genet. 6, 58-62.
Davis M.E. and Simmen R.C.M. (1997). Genetic parameter estimates for serum insulin-like growth factor concentration and performance traits in Angus beef cattle. J. Anim. Sci. 75, 317-324.
Eulalia S., Lech Z., Jolanta O., Nina S., Emilia B. and Krzyzewski J. (2006). Effect of polymorphism in IGF-I gene on production traits in Polish Holstein-Friesian cattle. Anim. Sci. Pap. Rep. 24, 225-237.
Ge W., Davis M.E., Hines H.C., Irvin K.M. and Simmen R.C.M. (2001). Associations of a genetic marker with blood serum insulin-like growth factor-I concentration and growth traits in Angus cattle. J. Aim. Sci. 79, 1757-1762.
Hines H.C., Ge W., Zhao Q. and Davis M.E. (1998). Association of genetic markers in growth hormone and insulin-like growth factor I loci with lactation traits in Holsteins. Anim. Genet. 29, 69-74.
Islam K.K., Vinsk M., Crews R.E., Okine E., Moore S.S., Crews Junior D.H. and Li C. (2009). Association analyses of a SNP in the promoter of IGF-I with fat deposition and carcass merit traits in hybrid, Angus and Charolais beef cattle. Anim. Genet. 40, 766-769.
Kim E.S., Shi X., Cobanoglu O., Weigel K., Berger P.J. and Kirkpatrick B.W. (2009). Refined mapping of twinning-rate quantitative trait loci on bovine chromosome 5 and analysis of insulin-like growth factor-I as a positional candidate gene. J. Anim. Sci. 87, 835-843.
Li C., Basarab J., Snelling W.M., Benkel B., Murdoch B., Hansen C. and Moore S.S. (2004). Assessment of positional candidate genes myf5 and IGF-I for growth on bovine chromosome 5 in commercial lines of Bos taurus. J. Anim. Sci. 82, 1-7.
Miller S.A., Dykes D.D. and Polesky H.F. (1988). A simple salting out procedure for extraction DNA from human nucleotide cells. Nucl. Acid. Res. 6, 3-4.
Moody D.E., Pomp D., Newman S. and Macheil M.D. (1996). Characterization of DNA polymorphisms in three populations of Hereford cattle and their associations with growth and maternal EPD in line 1 Herefords. J. Anim. Sci. 74, 1784-1793.
Moyes T.E. (2004). Variation in concentrations of insulin-like growth factor-I (IGF-I) in pasture-fed Holstein-Friesian cows. Ph D. Thesis. University of Melbourne, Melbourne, Australia.
Owens F.N., Dubeski P. and Hanson C.F. (1993). Factors that alter the growth and development of ruminants. J. Anim. Sci. 71, 3138-3150.
Rajkumar K., Modric T. and Murphy L.J. (1999). Impaired adipogenesis in insulin-like growth factor binding protein-I transgenic mice. J. Endocrinol. 162, 457-465.
SAS Institute. (2004). SAS^®/STAT Software, Release 6.1. SAS Institute, Inc., Cary, NC.
Schwerin M., Brockmann G., Vanselow J. and Seyfert H.M. (1995). Perspectives of molecular genome analysis in livestock improvement. Arch. Fur. Tierz. 38, 21-31.
Shen W., Wisniowski P., Ahmed L., Boyle D.W. Denne S.C. and Liechty E.A. (2003). Protein anabolic effects of insulin and IGF-I in the ovine fetus. Am. J. Physiol. Endocrinol. Metab. 284, 48-56.
Sirotkin A.V., Chrenek P., Makarevich A.V., Huba J. and Bulla J. (2000). Interrelationships between breed, growth hormone, plasma IGF-I level and meat performance in bulls of different ages. Arch. Fur. Tierz. 6, 591-596.
Yao J., Aggrey S.E., Zadworny D., Hayes J.F. and Kuhnlein U. (1996). Sequence variations in the bovine growth hormone gene characterized by single-strand conformation polymorphism (SSCP) analyses and their association with milk production traits in Holstein. Genetic. 144, 1809-1816.
Yilmaz A., Davis M.E., Hines C.H. and Chung H. (2005). Detection of two nucleotide substitutions and putative promoters in the 5’ flanking region of the ovine IGF-I gene. J. Appl. Genet. 46, 307-309.
Zhang Y., Du H., Chen J., Yang G. and Zhang X. (2008). Porcine growth differentiation factor 9 gene polymorphisms and their associations with litter size. J. Genet. Genomic. 35, 163-169.