Intestinal Histomorphology Changes and Serum Biochemistry Responses of Broiler Chickens Fed Herbal Plant (Euphorbia hirta) and Mix of Acidifier

Subject Areas : Camel

س.ر.  هاشمی ¹ (Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)
ا.  زولکیفلی ² (Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, Malaysia)
ه.  داودی ³ (Department of Microbiology and Immunology, Golestan University of Medical Science, Gorgan, Iran)
م.  هیر بجو ⁴ (Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia)
ت.س.  لوه ⁵ (Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, Malaysia)

Received: 2012-09-23 Accepted : 2013-02-12 Published : 2014-03-01

Keywords: Broiler, gut morphology, Euphorbia hirta, feed additive, herbal plants,

Abstract :

The aim of this study was to evaluate the effect of dietary Euphorbia hirta and an acidifier mixture supplementation on gut morphology and some blood parameters of broiler chickens.A total of 240 day old male broiler chicks were randomly assigned to one ofthe four dietary treatment groups including: (1) basal diet (control), (2) basal diet + 7.5 g/kg E. hirta (Eh7.5), (3) basal diet + 1.5 g/kg acidifier (OA) and (4) basal diet + 7.5 g/kg E. hirta and1.5 g/kg acidifier (EhOA). The Eh 7.5, OA and EhOA supplementation significantly improved overall feed conversion ratio compared to the control group. The addition ofEh 7.5, OA and their combination increased the villus height compare to the control birds. Crypt depth was markedly decreased by OA treatment. The highest ratio of villi to crypt was observed in OA fed broilers. Blood serum biochemical parameters did not influenced by the dietary treatments. In conclusion, the results indicated thataddition of Eh 7.5 and OA to the broiler diet enhanced maintenance and function of the small intestine and broiler performance.

References:

Abdel Fattah S.A., El Sanhoury M.H., El Mednay N.M. and Abdel Azeem F. (2008). Thyroid activity, some blood constituents, organs morphology and performance of broiler chicks fed supplemental organic acids. Int. J. Poult. Sci. 7, 215-222.
AdibmoradiM., Navidshad B., Seifdavati J. and Royan M. (2006). Effect of dietary garlic meal on histological structure of small intestine in broiler chickens. Poult. Sci. 43, 378-383.
Ao T.A. Cantor H., Pescatore A.J., Ford M.J., Pierce J.L. and Dawson K.A. (2009). Effect of enzyme supplementation and acidification of diets on nutrient digestibility and growth performance of broiler chicks. Poult. Sci. 88, 111-117.
Aptekmann K.P., Baraldi Arton S.M., Stefanini M.A. and Orsi M.A. (2001). Morphometric analysis of the intestine of domestic quails (Coturnix coturnix japonica) treated with different levels of dietary calcium. Anat. Histol. Embryol. 30, 277-280.
Awad W., Ghareeb K. and Böhm J. (2008). Intestinal structure and function of broiler chickens on diets supplemented with a synbiotic containing Enterococcus faecium and oligosaccharides. Int. J. Mol. Sci. 9, 2205-2216.
Bi Y.U. and Chiou P.W.S. (1996). Effects of crude fibre level in the diet on the intestinal morphology of growing rabbits. Lab. Anim. 30, 143-148.
Canibe N., Engberg R.M. and Jensen B.B. (2001). An Overview of the Effect of Organic Acids on Gut Flora and Gut Health. Danish Institute of Agricultural Science. Research Center Foulum, Denmark.
Choct M. (2009). Managing gut health through nutrition. Br. Poult. Sci. 7, 9-15.
Cowan M.M. (1999). Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12, 564-582.
Cook R.H and Bird F.H. (1973). Duodenal villus area and epithelial cellular migration in conventional and germ-free chicks. Poult. Sci. 52, 2276-2280.
Draughon F.A. (2004). Use of botanicals as biopreservatives in foods. Food. Technol. 58, 20-28.
Fulton R.M., Nersessian B.N. and Reed W.M. (2002). Prevention of Salmonella enteritidis infection in commercial ducklings by oral chicken egg derived antibody alone or in combination with probiotics. Poult. Sci. 81, 34-40.
Frankel W.L., Zhang W., Singh A., Klurfeld D.M., Don S., Sakata T. and Rombeau J.L. (1994). Mediation of the trophic effects of short chain fatty acids on the rat jejunum and colon. Gastroenterology. 106, 385-390.
Fukunaga T.M., Sasaki Y., Araki T., Okamoto T., Yasuoka T., Tsujikawa T., Fujiyama Y. and Bamba T. (2003). Effects of the soluble fiber pectin on intestinal cell proliferation, fecal short chain fatty acid production and microbial population. Digestion. 67, 42-49.
Garcia V., Catala Gregori P., Hernandez F., Megias M.D. and Madrid J. (2007). Effect of formic acid and plant extracts on growth, nutrient digestibility, intestine mucosa morphology and meat yield of broilers. J. Appl. Poult. Res. 16, 555-562.
Griggs J.P. and Jacob J.P. (2005). Alternatives to antibiotics for organic poultry production. J. Appl. Poult. Res.14, 750-756.
Guo F.C., Williams B.A., Kwakkel R.P., Li H.S., Li X.P., Luo J.Y., Li W.K. and Verstegen M.W.A. (2004). Effects of mushroom and herb polysaccharides, as alternatives for an antibiotic, on the cecal microbial ecosystem in broiler chickens. Poult. Sci.83, 175-182.
Hashemi S.R., Zulkifli I., Hair Bejo M., Farida A. and Somchit M.N. (2008a). Acute toxicity study and phytochemical screening of selected herbal aqueous extract in broiler chickens. Int. J. Pharmacol. 4, 352-360.
Hashemi S.R., Zulkifli I., Zunita Z. and Somchit M.N. (2008b). The effect of selected sterilization methods on antibacterial activity of aqueous extract of herbal plants. J. Biol. Sci. 8, 1072-1076.
Hashemi S.R., Zulkifli I., Hair Bejo M., Karami M. and Soleimani A.F. (2009a). The effects of Euphorbia hirta and acidifier supplementation on growth performance and antioxidant activity in broiler chickens. Pp. 215-217 in Proc. 21^st Vet. Assoc. Malaysia (VAM) Cong., Port Dickson, Malaysia.
Hashemi S.R., Zulkifli I., Zunita Z., Hair Bejo M., Loh T.C., Somchit M.N., Kok P.C. and Davoodi H. (2009b). Effects of dietary supplementation with Euphorbia hirta and acidifier on performance and Salmonella colonization in broiler chickens. Pp. 69-70 in Proc. 30^th Malaysia Soc. Anim. Prod. Annu. Conf., Kota Kinabalu, Malaysia.
Hashemi S.R. (2010). Selected herbal plants as growth and health promoters in broiler chickens. Ph D Thesis. Univ., Putra Malaysia (UPM), Malaysia.
Hashemi S.R and Davoodi H. (2010) Phytogenics as new class of feed additive in poultry industry. J. Anim. Vet. Adv. 9,2955-2304.
Hashemi S.R and Davoodi H. (2011) Herbal plants and their derivatives as growth and health promoters in animalnutrition.Vet. Res. Commun. 35, 169-180.
Hermes C., Azevedo J.F., Araujo E.J.A. and Santana D.M.G. (2008). Intestinal ascending colon morphometrics in rats submitted to severe protein malnutrition. Int. J. Morphol. 26, 5-11.
Hughes R.J. (2003). Energy Metabolism of Chickens Physiological Limitations. A report for the Rural Industries Research and Development Corporation, RIRDC Publication.
Jamroz D., Orda J., Kamel C., Wiliczkiewicz A., Wertelecki T. and Skorupinska J. (2003). The influence of phytogenic extracts on performance, nutrient digestibility, carcass characteristics and gut microbial status in broiler chickens. J. Anim. Feed Sci.12, 583-596.
Kawalilak L.T., Ulmer Franco A.M. and Fasenko G.M. (2011). Impaired intestinal villi growth in broiler chicks with unhealed navels. Poult. Sci. 89, 82-87.
Kirchgessner M., Roth F.X. and Steinruck U. (1991). Nutritive wirkung von fumarsäure bei anderung der proteinqualität und des proteingehaltes im futter auf die mastleistung von broilern. Arch. Geflügelkd. 55, 224-232.
Kum S., Eren U., Onol A.G. and Sandikci M. (2010). Effects of dietary organic acid supplementation on the intestinal mucosa in broilers. Rev. Med. Vet. 10, 463-468.
Langhout P. (2000). New additives for broiler chickens. World's Poult. 16, 22-27.
Le Blay G., Blottiere H.M., Ferrier L., Le Foll E., Bonnet C., Galmiche J.P. and Cherbut C. (2000). Short-chain fatty acids induce cytoskeletal and extracellular protein modification associated with modulation of proliferation on primary culture of rat intestinal smooth muscle cells. Dig. Dis. Sci. 45, 1623-1630.
Loddi M.M., Maraes V.M.B., Nakaghi I.S.O., Tucci F., Hannas M.I. and Ariki J.A. (2004). Mannan oligosaccharide and organic acids on performance and intestinal morphometric characteristics of broiler chickens. Pp. 45 in Proc. 20^th Annu. Symp. Comp. Geom., Brooklyn, NY.
Luckstadt C., Nizamettin S., Hasan A. and Aylin A. (2004). Acidifier a modern alternative for antibiotic free feeding in livestock production, with special focus on broiler production. Vet. Zootech. 27, 91-95.
Miles R.D. Janky D.M. and Harms R.H. (1984). Virginiamycin and broiler performance. Poult. Sci. 63, 1218-1221.
Montagne L., Pluske J.R. and Hampson D.J. (2003). A review of interactions between dietary fibre and the intestinal mucosa,and their consequences on digestive health in young non-ruminant animals. Anim. Feed Sci. Technol. 108, 95-117.
Nabuurs M.J., Hoogendoorn A., Van Der Molen E.J. and Van Osta A.L.M. (1993). Villus height and cryp depth in weaned pigs reared under various circumstances in the Netherland. Res. Vet. Sci. 55, 78-84.
NRC. (1994). Nutrient Requirements of Poultry, 9^th Rev. Ed. National Academy Press, Washington, DC.
Ogbulie J.N., Ogueke C.C., Okoli I.C. and Anyanwu1 B.N. (2007). Antibacterial activities and toxicological potentials of crude ethanolic extracts of Euphorbia hirta. African J. Biotechnol. 6, 1544-1548.
Owens B., Tucker L., Collins M.A. and Mc Cracken K.J. (2008). Effects of different feed additives alone or in combination on broiler performance, gut microflora and ileal histology. Br. Poult. Sci. 49, 202-212.
Papatsiros V.G., Christodoulopoulos G. and Filippopoulos L.C. (2012). The use of organic acids in monogastric animals (swine and rabbits). J. Cell Anim. Biol. 6, 154-159.
Patte J.D. and Waldroup P.W. (1998). Use of organic acids in broiler diets. Poult. Sci. 67, 1178-1182.
Paul S.K., Halder G., Mondal M.K. and Samanta G. (2007). Effect of organic acid salt on the performance and gut health of broiler chicken. Poult. Sci. 44, 389-395.
Pelicano E.R.L., Souza P.A., Souza H.B.A., Figueiredo D.F., Boiagom M., Carvalho S.R. and Bordon V.F. (2005). Intestinal mucosa development in broiler chicken fed natural growth promoters. Brazilian J. Poult. Sci. 4, 221-229.
Radecki S.V. and Yokoyama M.T. (1991) Intestinal bacteria and their influence on swine nutrition. Pp. 439-447 in Swine Nutrition. E.R. Miller, E.U. Duane and A.J. Lewis, Eds. Boston: Butter Worth Heinemann.
Ratcliff J. (2000). Antibiotic bans A European perspective. Pp. 135-152 in Proc. 47^th Maryland Nutr. Conf., Feed Manufac. Univ. Maryland, College Park.
Roser U. (2006) Effects of organic acids in liquid and solid forms on the survival rate of Salmonella in pelleted compound feed after recontamination. Immunology. 82, 12-19.
Sakamoto K., Hirose H., Onizuka A., Hayashi M., Futamura N., Kawamura Y. and Ezaki T. (2000). Quantitative study of changes in intestinal morphology and mucus gel on total parenteral nutrition in rats. J. Surg. Res. 94, 99-106.
SAS. (2005). SAS^®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC.
Scheppach W., Bartram H.P. and Richter F. (1995). Role of short-chain fatty acids in the prevention of colorectal cancer. European J. Cancer. 31, 1077-1080.
Schneeman B.D. (1982). Pancreatic and digestive function. Pp. 73-83 in Dietary Fibre in Health and Disease. G.V. Vahuoung and D. Kritchevsky, Eds. Plenum Press, New York.
Sharma N.K., Dey S. and Prasad R. (2007). In vitroantioxidant potential evaluation of Euphorbia hirta. Pharmacologyonline.1, 91-98.
Silva M.A., Pessotti B.M.S., Zanini S.F., Colnago G.L., Rodrigues M.R.A., Nunes L.C., Zanini M.S. and Martins I.V.F. (2009). Intestinal mucosa structure of broiler chickens infected experimentally with Eimeria tenella and treated with essential oil of oregano. Cienc. Rural. 5, 1471-1477. 
Skinner J.T., Izat A.L. and Waldroup P.W. (1991). Research note: fumaric acid enhances performance of broiler chickens. Poult. Sci. 70, 1444-1447.
Skrivanova E. and Marounek M. (2007). Influence of pH on antimicrobial activity of organic acids against rabbit enteropathogenic strain of Escherichia coli. Folia Microb. 52, 70-72.
Solisde los Santos F., Tellez G., Farnell M.B., Balog J.M., Anthony N.B., Pavlidis H.O. and Donoghue A.M. (2005). Hypobaric hypoxia in ascites resistant and susceptible broiler genetic lines influences gut morphology. Poult. Sci. 84, 1495-1498.
Tappenden K.A. and McBurney M.I. (1998). Systemic short-chain fatty acids rapidly alter gastrointestinal structure, function and expression of early response genes. Dig. Dis. Sci. 43, 1526-1536.
Truscott R.B. and Al-Sheikhly F. (1997). The production and treatment of necrotic enteritis in broilers. Am. J. Vet. Res. 38, 857-861.
Vieira S.L., Oyarzabal O.A., Freitas D.M., Berres J., Pena J.E.M., Torres C.A. and Coneglian J.L.B. (2008). Performance of broilers fed diets supplemented with sanguinarine-like alkaloids and organic acids. J. Appl. Poult. Res. 17, 128-133.
Vijaya K., Ananthan S. and Nalini R. (1995). Antibacterial effect of theaflavin, polyphenon 60 (Camellia siensis) andEuphorbia hirta on Shigella spp. A cell culture study. J. Ethnopharmacol. 49, 115-118.
Vogt H., Matthes S. and Harnisch S. (1981). The effect of organic acids in the rations on the performance of broilers and laying hens. Arch. Geflugelkd. 45, 221-232.
Vogt H., Matthes S., Harnisch S. and Ristic M. (1979). Fumaric acid in broiler rations. Arch. Geflugelkd. 43, 54-60.
Waldroup A., Kaniawati S. and Mauromoustakos A. (1995). Performance characteristics and microbiological aspects of broiler fed diets supplemented with organic acids. J. Food Protec. 58, 482-489.
Williams L.A.D., Williams M.G., Sajabi A., Barton E.N. and Fleischhacker R. (1997). Angiotensin converting enzyme inhibiting and anti dipsogenic activities of Euphorbia hirta extracts. Phyto. Res. 11, 401-405.
Windisch W., Schedle K., Plitzner C. and Kroismayr A. (2008). Use of phytogenic products as feed additives for swine and poultry. J. Anim. Sci. 86, 140-148.
Yakhkeshi S., Rahimi S. and Gharib Naseri K. (2011). The effects of comparison of herbal extracts, antibiotic, probiotic and organic acid on serum lipids, immune response, GIT microbial population, intestinal morphology and performance of broilers. J. Med. Plants. 10, 80-95.
Yang Y., Iji P.A. and Choct M. (2009). Dietary modulation of gut microflora in broiler chickens: a review of the role of six kinds of alternatives to in-feed antibiotics. World's Poult. Sci. J. 65, 97-114.