Effect of Essential Oils of Zataria multiflora, Eucalyptus globolus and Their Combination on Fermentation Parameters Using Merghoz Goat Rumen Liquor

Subject Areas : Camel

س. میرزایی چشمه‌گچی ¹ , م.م. معینی ² , ف. هژبری ³ , م.ا. نوریان سرور ⁴

1 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran
2 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran
3 - عضو هیات علمی د انشگاه رازی
4 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran

Received: 2016-06-20 Accepted : 2016-10-15 Published : 2017-03-01

Keywords: partitioning factor, methane production, organic matter digestibility, protozoa popu-lation,

Abstract :

In this experiment, different doses of Zataria multiflora (ZM) and Eucalyptus globolus (EG) essential oils (EOs) (0, 100, 250, 500, 750 and 1000 µg/mL) and a mixture of them were added to alfalfa hay incubated with buffered rumen liquor of Merghoz goat to assess in vitro gas production, rumen fermentation and protozoa population. In all treatments the asymptotic gas volume (B) and the rate constant (c) were decreased while the lag phase was increased. There were no effects of ZM and EG on pH, but in high doses of ZE pH was higher than that of control (P<0.001). The ammonia-N concentration was decreased due to addition of combination of EOs (P<0.01) and volatile fatty acid concentration was reduced (P<0.01) following incorporation of EOs. Gas production and organic matter digestibility were decreased (P<0.01) 24 h after incubation, whereas the partitioning factor was increased. Metabolizable energy was decreased, (P<0.05). By inclusion of EOs, total protozoa population and individual genera reduced (P<0.001). The results revealed that EOs of ZM and EG could be potentially used to modulate rumen fermentation, but using them at high level doses have anti-protozoal effects.

References:

Barnett A.J.G. and Reid R.L. (1957). Studies on production of volatile fatty acids from grass by rumen liquid in an artificial rumen. J. Agric. Sci. 48, 315-321.

Beauchemin K.A. and Mcginn S.M. (2006). Methane emissions from beef cattle: effects of fumaric acid, essential oil and canola oil. J. Anim. Sci. 84, 1489-1496.

Benchaar C., Calsamiglia S., Chaves A.V., Fraser G.R., Colombatto D., McAllister T.A. and Beauchemin K.A. (2008). A review of plant-derived essential oils in ruminant nutrition and production. Anim. Feed Sci. Technol. 145, 209-228.

Benchaar C., Chaves A.V., Fraser G.R., Wang Y., Beauchemin K.A. and McAllister T.A. (2007). Effects of essential oils and their components on in vitro rumen microbial fermentation. Canadian J. Anim. Sci. 87, 413-419.

Blümmel M., Makkar H.P.S. and Becker K. (1997). In vitro gas production, a technique revisited. J. Anim. Physiol. Anim. Nutr. 77, 24-34.

British Pharmacopoeia. (1988). Her Majesty's Stationery Office. HMSO, London, UK.

Broderick G.A. and Kang J.H. (1980). Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J. Dairy Sci. 63, 64-75.

Cardozo P.W., Calsamiglia S., Ferret A. and Kamel C. (2006). Effects of alfalfa extract, anise, capsicum and a mixture of cinnamaldehyde and eugenol on ruminal fermentation and protein degradation in beef heifers fed a high concentrate diet. J. Anim. Sci. 84, 2801-2808.

Castillejos L., Calsamiglia S., Ferret A. and Losa R. (2007). Effects of dose and adaptation time of a specific blend of essential oil compounds on rumen fermentation. Anim. Feed Sci. Technol. 132, 186-201.

Dehority B.A. (1993). Laboratory Manual for Classification and Morphology of Rumen Ciliate Protozoa. CRC Press, Boca Raton, Florida, USA.

Demeyer D.I., Meulemeester M., de Graeve K. and Gupta B.W. (1988). Effect of fungal treatment of nutritive value of straw. Med. Fac. Landbouww. Rijksuniv. Gent. 53, 1811-1819.

Fievez V., Babayemi O.J. and Demeyer D. (2005). Estimation of direct and indirect gas production in syringes: a tool to estimate short chain fatty acid production that requires minimal laboratory facilities. Anim. Feed Sci. Technol. 123, 197-210.

Fraser G.R., Chaves A.V., Wang Y., McAllister T.A., Beauchemin K.A. and Benchaar C. (2007). Assessment of the effects of cinnamon leaf oil on rumen microbial fermentation using two continuous culture systems. J. Dairy Sci. 90, 2315-2328.

Gallucci M.N., Oliva M., Casero C., Dambolena J., Luna A. and Zygadlo J. (2009). Antimicrobial combined action of terpenes against the food-borne microorganisms Escherichia coli, Staphylococcus aureus and Bacillus cereus. Flavour Frag. J. 24, 348-354.

Garcia-González R., Lopez S., Fernandez M. and Gonzalez J.S. (2008). Dose response effects of Rheum officinale root and Frangula alnus bark on ruminal methane production in vitro. Anim. Feed Sci. Technol. 145, 319-334.

Goel G., Puniya A.K., Aguliar C.N. and Singh K. (2005). Interaction of gut microflora with tannins in feeds. Naturwissenschaften. 92, 497-503.

Jahani-Azizabadi H., Danesh Mesgaran M., Vakili A. and Rezayazdi K. (2014). Effect of some plant essential oils on in vitro ruminal methane production and on fermentation characteristics of a mid-forage diet. J. Agric. Sci. Technol. 16, 1543-1554.

Joch M., Cermak L., Hakl J., Hucko B., Duskova D. and Marounek M. (2016). In vitro screening of essential oil active compounds for manipulation of rumen fermentation and methane mitigation. Asian-Australas J. Anim. Sci. 29(7), 952-959.

Lopez S., Makkar H.P.S. and Soliva C.R. (2010). Screening plants and plant products for methane inhibitors. Pp. 191-231 in In vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies. P.E. Vercoe, H.P.S. Makkar and A.C. Schlink, Eds. Springer, Dordrecht, the Netherlands.

Macheboeuf D., Morgavi D.P., Papon Y., Mousset J.L. and Arturo-Schaan M. (2008). Dose-response effects of essential oils on in vitro fermentation activity of the rumen microbial population. Anim. Feed Sci. Technol. 145, 335-350.

Maciel M.V., Morais S.M., Bevilaqua C.M.L., Silva R.A., Barros R.S., Sousa R.N., Sousa L.C., Brito E.S. and Souza-Neto M.A. (2010). Chemical composition of Eucalyptus spp. essential oils and their insecticidal effects on Lutzomyia longipalpis. Vet. Parasitol. 167, 1-7.

Makkar H.P.S. (2010). In vitro screening of feed resources for efficiency of microbial protein synthesis. Pp. 107-144 in In vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies. P.E. Vercoe, H.P.S. Makkar and A.C. Schlink Eds. Springer, Dordrecht, Netherlands.

McAllister T.A., Bae H.D., Jones G.A. and Cheng K.J. (1994). Microbial attachment and feed digestion in the rumen. J. Anim. Sci. 72, 3004-3018.

Mcdonald P., Edwards R.A., Greenhalgh J.F.D., Morgan C.A., Sinclair L.A. and Wilkinson R.G. (2010). Animal Nutrition. Prentice Hall, London, UK.

Mcintosh F.M., Williams P., Losa R., Wallace R.J., Beever D.A. and Newbold C.J. (2003). Effects of essential oils on ruminal microorganisms and their protein metabolism. Appl. Environ. Microbiol. 69, 5011-5014.

Menke K.H. and Steingass H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev. 28, 7-55.

Mirzaei Z., Hozhabri F. and Alipour D. (2016). Thymus kotschyanus essential oil component and their effects on in vitro rumen fermentation, protozoal population and acidosis parameters. Iranian J. Appl. Anim. Sci. 6(1), 53-58.

Newbold C.J., McIntosh F.M., Williams P., Losa R. and Wallace R.J. (2004). Effects of a specific blend of essential oil compounds on rumen fermentation. Anim. Feed Sci. Technol. 114, 105-112.

Ørskov E.R. and McDonald I. (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. 92, 499-503.

Sallam S.M.A., Bueno I.C.S., Brigide P., Godoy P.B., Vitti D.M.S.S. and Abdalla A.L. (2009). Efficacy of eucalyptus oil on in vitro rumen fermentation and methane production. Options Mediterraneennes. 85, 267-272.

Spanghero M., Zanfi C., Fabbro E., Scicutella N. and Camellini C. (2008). Effects of a blend of essential oils on some end products of in vitro rumen fermentation. Anim. Feed Sci. Technol. 145, 364-374.

SPSS Inc. (2015). Statistical Package for Social Sciences Study. SPSS for Windows, Version 23.0. Chicago SPSS Inc.

Taghavi-Nezhad M., Alipour D., Flythe M.D., Zamani P. and Khodakaramian G. (2014). The effect of essential oils of Zataria multiflora and Mentha spicata on the in vitro rumen fermentation, and growth and deaminative activity of amino acid-fermenting bacteria isolated from Mehraban sheep. J. Anim. Prod. Sci. 54, 299-307.

Taghavi-Nezhad M., Alipour D., TorabiGoudarzi M., Zamani P. and Khodakaramian G. (2011). Dose response to carvone rich essential oils of spearmint (Mentha spicata): in vitro ruminal fermentation kinetics and digestibility. J. Agric. Sci. Technol. 13, 1013-1020.

Talebzadeh R., Alipour D., Saharkhiz M.J., Azarfar A. and Malecky M. (2012). Effect of essential oils of Zataria multiflora on in vitro rumen fermentation, protozoal population, growth and enzyme activity of anaerobic fungus isolated from Mehraban sheep. Anim. Feed Sci. Technol. 172, 115-124.

Wallace R.J., McEwan N.R., McIntosh F.M., Teferedegne B. and Newbold C.J. (2002). Natural products as manipulators of rumen fermentation. Asian-Australas J. Anim. Sci. 15, 1458-1468.

Williams A.G. and Coleman G.S. (1992). The Rumen Protozoa. Springer-Verlag, London, UK.

Yang W.Z., Ametaj B.N., Benchaar C. and Beauchemin K.A. (2010). Dose response to cinnamaldehyde supplementation in growing beef heifers: ruminal and intestinal digestion. J. Anim. Sci. 88, 680-688.