Dry Matter and Crude Protein Degradability ofMopane Worm (Imbrasia belina) in Rumen of Steers

Subject Areas : Camel

O.R. Madibela ¹ , B. Giddie ² , K.K. Mokwena ³

1 - Department of Animal Science and Production, Botswana College of Agriculture, P / bag 0027, Gaborone, Botswana
2 - Department of Animal Science and Production, Botswana College of Agriculture, P / bag 0027, Gaborone, Botswana
3 - Department of Agricultural Engineering and Land Planning, Botswana College of Agriculture, P / bag 0027, Gaborone, Botswana

Received: 2012-01-05 Accepted : 2012-04-01 Published : 2013-03-01

Keywords: effective degradability, mopane worm, outflow rates, Tswana steers,

Abstract :

Three cannulated Tswana steers were used to investigate the rumen degradability of mopane worm (Imbrasia belina) by measuring the amount of dry matter (DM) and crude protein (CP) disappearing at incubation periods up to 72 h. The effective degradability (ED) of DM and CP in the rumen was calculated at outflow rates of 0.03 / h (ED0.03) and 0.05 / h(ED0.05). Rumen degradable CP (RDP) was estimated as the amount of CP x ED 0.05 and rumen undegraded protein (RUP) was calculated as CP minus RDP. The soluble DM, slowly degradable DM, ED0.03 and ED 0.05,and degradation rate of evacuated (i.e. fresh leaves and faecal material removed) mopane (EM) and intact mopane (IM) were similar (P>0.05). Water soluble protein fraction, slowly degradable protein, degradation rate and RDP were also similar (P>0.05) between EM and IM samples. However, EM had significantly more (P<0.01) RUP than IM samples. The current results support previously observations that removal of gut contents from mopane worms increased CP, consequently improving RUP. Further research is needed to evaluate the effects of roasting on degradability of EM and IM as well as the availability of amino acids post-ruminally.

References:

AOAC. (1990). Official Methods of Analysis. Vol. I. 15^th Ed. Association of Official Analytical Chemists, Arlington, VA.
Barry T.N. (1985). The role of condensed tannins in the nutritional value of Lotus pedunculatus for sheep. 3. Rates of body and wool growth. Brit. J. Nutr. 54, 211-217.
Ben Salem H., Makkar H.P.S., Nefzaoui A., Hassayoun L. and Abidi S. (2005).Benefit from the association of small amounts of tannin-rich shrub foliage (Acacia cyanophylla Lindl.) with soya bean meal given as supplements to Barbarine sheep fed on oaten hay. Anim. Feed Sci. Technol. 122, 173-186.
CIOMS. (1985). International Guidelines for Biomedical Research Involving Animals. Council for International Organisation of Medical Science, Geneva.
Ditlhogo M. (1996).The ecology of Imbrasia belina (Westwood) in north-Eastern Botswana. Pp. 201-211 in Proc. Multidisciplinary Symposium on Mopane. B.A. Gashe and S.F. Mpuchane Eds. University of Botswana and Kalahari Conservation Society. Gaborone, Botswana.
El-Hassan S.M., Lahlou A., Kassi A., Newbold C.J. and Wallace R.J. (2000). Chemical composition and degradation characteristics of foliage of some African multipurpose trees. Anim. Feed Sci. Technol. 86, 27-37.
Erasmus L.J., Botha P.M. and Cruywagen C.W. (1994). Amino acid profile and intestinal digestibility in dairy cows of rumen-undegradable protein from various feedstuffs. J. Dairy Sci. 77, 541-551.
Hovell F., Deb D., Ngambi J.W.W., Barber W.P. and Kyle D.J. (1986). The voluntary intake of hay by sheep in relation to its degradability in the rumen as measured in nylon bags. Anim. Prod. 42, 111-118.
Kamalak A., Canbolat O., Gurbuz Y. and Ozay O. (2005). In situ ruminal dry matter and crude protein degradability of plant- and animal-derived protein sources in Southern Turkey. Small Rum. Res. 58, 135-141.
Madibela O.R., Seitiso T.K., Thema T.F. and Letso M. (2007). Effect of traditional processing methods on chemical composition and in vitro true dry matter digestibility of Mopane worm (Imbrasia belina). J. Arid. Env. 68, 492-500.
Madibela O.R., Mokwena K.K., Nsoso S.J. and Thema T.F. (2009). Chemical composition of Mopane worm sampled at three sites in Botswana and subjected to different processing. Trop. Anim. Health. Prod. 41, 935-942.
Maiga H.A., Schingoethe D.J. and Henson J.E. (1996). Ruminal degradation, amino acid composition, and intestinal digestibility of the residual components of five protein supplements. J. Dairy Sci. 79, 1647-1653.
Marais E. (1996).Omaungu in Nambia: Imbrasia beline (Saturniidae: Lepidoptera) as a commercial resource. Pp. 23-31 in Proc. 1^st Multidisciplinary Symp. Mopane, University of Botswana and Kalahari Conservation Society. Gaborone, Botswana.
Marichal M., Carriquiry M., Pereda R. and San Martin R. (2000). Protein degradability and intestinal digestibility of blood meals: comparison of two processing methods. Anim. Feed Sci. Technol. 88, 91-101.
McDonald P., Edwards R.A., Greenhalgh J.F.D. and Morgan C.A. (2002). Animal Nutrition, 6^th Ed. Pearson Education Limited. Essex.
Mehrez A.Z. and Ørskov E.R. (1977). A study of the artificial fiber bag technique for determining the digestibility of feeds in the rumen. J. Agric. Sci. 88, 645-650.
Ministry of Agriculture (1998). Stock Feed of ruminant origin ban regulations, statutory instrument in Diseases of Animals Act. Gaborone, Botswana.
Moruakgomo M.B.W. (1996).Commercial utilization of Botswana’s veld products-The economics of mopane – the dimensions of mopane trade. Pp. 182-200 in Proc. Multidisciplinary Symposium. Mopane. University of Botswana and Kalahari Conservation Society. Gaborone, Botswana.
Mpuchane S., Gashe B.A., Allotey J., Siame B., Teferra G. and Ditlhogo M. (2000). Quality deterioration of mopane, the edible caterpillar of an emperor moth Imbrasia belina. Food Control. 11, 453-458.
Ohiokpehai O., Bulawayo B.T., Mpotokwane S., Sekwati B. and Bertinuson A. (1996) Expanding the use of mopane, a nutritionally rich local food. Pp. 111-130 in Proc. Multidisciplinary Symposium. Mopane. University of Botswana and Kalahari Conservation Society. Gaborone, Botswana.
Ørskov E.R. (1992). Protein Nutrition in the Ruminant. 2^nd Edition Academic Press, London.
Ørskov E.R. and McDonald I. (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. 92, 499-503.
Ørskov E.R., Hovell F.D. and Mould F. (1980). The use of nylon bag technique for the evaluation of feedstuffs. Trop. Anim. Prod. 5, 195-213.
Osuji P.O., Nsahlai I.V. and Khalili H. (1993). Feed Evaluation. Aka ILCA and ILRAD. http://www.ilri.org/html/trainingMat/FEEDMAN.PDF.Accessed Feb. 2011
Rasengwatshe A. and Madibela O.R. (2005). Rumen degradability of Imbrasia belina (Mopane worm) and carcass meal. J. Anim. Vet. Sci. 4, 1021-1024.
SAS Institute Inc. (2004). SAS User’s Guide. Release 6. SAS Institute Inc. Cary, NC, USA.
Siame A.B., Mpuchane S.F., Gashe B.A., Allotey J. and Teferra G. (1996). Nutritional quality of Mopane worms, Imbrasia belina (Westwood), and the microorganisms associated with the worms. Pp. 89-97 in Proc. Multidisciplinary Symposium. B.A. Gashe and S.F. Mpuchane Eds. University of Botswana and Kalahari Conservation Society. Gaborone, Botswana.
Sokal R.R. and Rohlf F.J. (1969). Biometry. The principles and Practice of Statistics in Biological Research. 2^nd Ed. WH Freedman, San Francisco, CA, USA. Pp. 222-229.
Styles C.V. (1996).The biological ecology of Imbrasia belina (saturniinae, saturniidae) with reference to its behaviour, physiology, distribution, population dynamics, impact within mopane veld and utilisation within South Africa. In Proc. Multidisciplinary Symposium. University of Botswana and Kalahari Conservation Society of Mopane. Gaborone, Botswana.
Titgemeyer E.C., Merchen N.R. and Berger L.L. (1989). Evaluation of soybean meal, corn gluten meal, blood meal and fish meal as sources of nitrogen and amino acids disappearing from the small intestine of steers. J. Anim. Sci. 67, 262-275.