• صفحه اصلی
  • بررسی فیتوشیمیایی و اثر ضد میکروبی عصارۀ گیاه دارویی Moringa oleifera Lam. تکثیر یافته از طریق کشت بافت

اشتراک گذاری

آدرس مقاله


کد مقاله : EJMP-2112-1667 (R1) بازدید : 165 صفحه: 77 - 94

10.30495/ejmp.2022.1947044.1667

20.1001.1.23223235.1401.10.4.5.5

نوع مقاله: پژوهشی

بررسی فیتوشیمیایی و اثر ضد میکروبی عصارۀ گیاه دارویی Moringa oleifera Lam. تکثیر یافته از طریق کشت بافت

محورهای موضوعی : فیتوشیمی

آسیه اسحاق زاده 1 , محبوبه طاهرخانی 2

1 - گروه فیتوشیمی و شیمی فناوری اسانس، دانشکده شیمی دارویی، واحد علوم دارویی، دانشگاه آزاد اسلامی، تهران، ایران.
2 - گروه شیمی، دانشکده علوم پایه، واحد تاکستان، دانشگاه آزاد اسلامی، تاکستان، ایران.

تاریخ دریافت : 1400/09/21 تاریخ پذیرش : 1401/01/18 تاریخ انتشار : 1401/10/01

کلید واژه: کشت بافت, استخراج, کوئرستین, آنتی باکتریال, گز روغنی,

چکیده مقاله :

گیاه گز روغنی (Moringa oleifera Lam) یک درخت گرمسیری متعلق به خانوادۀ Moringaceae و دارای 14 گونه در آفریقا، ماداگاسکار، آسیای غربی و شبه قاره هند است. این تحقیق به بررسی فیتوشیمیایی و اثرات ضد میکروبی عصارۀ گیاه M. oleifera تکثیر یافته از طریق کشت درون شیشه ای در ایران می پردازد. در بهار سال 1395، با کشت بافت اثرات محیط را بر روی مقدار و میزان ترکیبات فیتوشیمیایی حذف کردیم و بدینصورت از توان و پتانسیل ژنتیکی گیاه آگاه شدیم. عصارۀ گیاه کشت بافتی به روش خیساندن در حلال، استخراج و نهایتاٌ ترکیب کوئرستین به عنوان یک فلاونوئید از طریق کروماتوگرافی ستونی و لایه نازک، استخراج، خالص سازی و سپس از طریق طیف سنجی رزونانس مغناطیسی هسته و تکنیک مادون قرمز شناسایی و تعیین ساختار مولکولی گردید. همچنین اثر آنتی میکروبی عصارۀ تام این گیاه توسط خیساندن در سه حلال متانول، کلروفرم و اتیل استات علیه چهار سویه باکتریایی Staphylococcus aureus ،Bacillus subtilis ، Pseudomonas aeruginosa و Escherichia coli به روش چاهک پلیت در محیط مایع مورد بررسی قرار گرفت و در نهایت حداقل غلظت مهار کنندگی رشد (MIC) و حداقل غلظت کشندگی باکتری (MBC) برای سویه های مذکور تعیین گردید. بزرگترین هاله عدم رشد مربوط به عصارۀ متانولی و به میزان 20 میلی متر بدست آمد. مؤثرترین نتیجه با توجه به مقادیرMIC و MBC مربوط به تأثیر عصاره متانولی بر روی سویۀ E. coli با غلظت 40 mg/ml بدست آمد. در نتیجه عصارۀ گیاه M. oleifera منبع غنی از مواد آنتی اکسیدانی نظیر فلاونوئید ها و همچنین عصارۀ متانولی آن دارای خاصیت آنتی میکروبی قابل توجهی می باشد.

چکیده انگلیسی:

Moringa oleifera Lam. is a tropical tree belonging to the family Moringaceae with 14 species. This study investigated the phytochemical and antimicrobial effects of M. oleifera extract propagated through tissue culture in Iran in the spring of 2016. The extract of plant obtained from tissue culture was extracted by maceration in a solvent, and purified quercetin as a natural compound was extracted through a column and thin layer chromatography, then the molecular structure of flavonoid was identified through Nuclear Magnetic Resonance spectroscopy and Infrared technique. To estimate the antibacterial activity, the total plant extract was obtained by Methanol, Chloroform, and Ethyl acetate and finally the Minimal Inhibitory Concentration (MIC) and the Minimal Bactericidal Concentration (MBC) were determined. The antimicrobial effect of this plant against four bacterial strains of Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli was investigated by plate well method in a liquid medium. The maximal inhibitory concentration (20 mm) was obtained from the methanolic extract. Also, the best results of MIC and MBC were obtained from the effect of 40 mg/ml of methanolic extract on the E. coli strain. Therefore, it is concluded that the extract of M. oleifera plant is a rich source of antioxidant compounds such as flavonoids, and also its methanolic extract has significant antimicrobial properties.

منابع و مأخذ:

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_||_

References

  1. Akintelu, S.A., Folorunso, A.S. and Oyebamiji, A.K. (2021). Phytochemical and antibacterial investigation of Moringa oleifera seed: experimental and computational approaches. Eclética Química Journal, 46(2): 17-25.
    2.
  2. Akya, A., Mojarab, M., Farshchian, M. and Ahmadi, K. (2014). The effect of stem bark extracts of Tamarix ramosissim shrub on Pseudomonas aeruginosa isolates. Scientific Journal of Kurdistan University of Medical Sciences, 19(4): 128-134.
    3.
  3. Amabye, T.G. and Tadesse, F.M. (2016). Phytochemical and antibacterial activity of Moringa Oleifera available in the market of mekelle. Journal of Analytical and Pharmaceutical Research, 2(1): 00011. DOI: 10.15406/japlr.2016.02.00011.
    4.
  4. Anwar, F., Latif, S., Ashraf, M. and Gilani, A.H. (2007). Moringa oleifera: a food plant with multiple medicinal uses. Phytotherapy Research, 21(1):17-25.
    5.


  1. Anzano, A., Ammar, M., Papaianni, M., Grauso, L., Sabbah, M., Capparelli, R. and Lanzotti, V. (2021). Moringa oleifera Lam.: a phytochemical and pharmacological overview. Horticulturae, 7, 409. https://doi.org/10.3390/horticulturae7100409.
    2.
  2. Asare, G.A., Gyan, B., Bugyei, K., Adjei, S., Mahama, R., Addo, P., Otu-Nyarko, L., Wiredu, E.K. and Nyarko, A. (2012). Toxicity potentials of the nutraceutical Moringa oleiferaat supra supplementation levels. Journal of Ethnopharmacology, 139(1): 265-272.
    3.
  3. Azizian Shermeh, O., Taherizadeh, M., Valizadeh, M. and Qasemi, A. (2018). Robial and antioxidant activities and determining phenolic and flavonoid contents of the extracts of five species from different families of the medicinal plants grown in Sistan and Baluchestan province. Journal of Fasa University of Medical Sciences, 7: 465-479.
    4.
  4. Bagheri, G., Martorell, M., Ramírez-Alarcón, K., Salehi, B. and Sharifi-Rad, J. (2020). phytochemical screening of Moringa oleifera leaf extracts and their antimicrobial activities. Cellular and Molecular Biology (Noisy-le-grand, France), 66(1): 20-26. PMID: 32359378.
    5.
  5. Balouiri, M., Sadiki, M. and Ibnsouda, S.K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2): 71-79.
    6.
  6. Barreto, M.B., Freitas, J.V.B.D., Silveira, E.R., Bezerra, A.M.E., Nunes, E.P. and Gramosa, N.V. (2009). Volatile and non-volatile chemical constituents of Moringa oleifera Lam., Moringaceae. Revista Brasileira de Farmacognosia, 19(4): 893-897.
    7.
  7. Bina, B., Shasavani, A., Asghare, G. and Hasanzade, A. (2007). Comparison of water turbidity removal efficiencies of Moringa oleifera seed extract and poly-aluminum chloride. Journal of Water and Wastewater, 18(1): 24-33.
    8.
  8. Biswas, D., Nandy, S., Mukherjee, A., Pandey, D.K. and Dey, A. (2020). Moringa oleifera Lam. and derived phytochemicals as promising antiviral agents: A review. South African Journal of Botany, 129: 272-282. https://doi.org/10.1016/j.sajb.2019.07.049.
    9.
  9. Daghaghele, S., Kiasat, A.R., safiodin ardebili, S.M., Mirzajani, R., (2022). Evaluation of different extraction methods of phytochemical and antioxidant compounds of Moringa oleifera leaf extract. Journal of food science and technology (FSCT), 18(121) :163-172.
    10.
  10. Daghaghele, S., Kiasat, A.R., Safieddin Ardebili,S.M., Mirzajani, R. (2021). Intensification of extraction of antioxidant compounds from Moringa Oleifera leaves Using ultrasound-assisted approach: BBD-RSM design. International Journal of Fruit Science, 21(1): 693-705.
    11.
  11. Dhakad, A.K., Ikram, M., Sharma, S., Khan, S., Pandey, V.V. and Singh, A. (2019). Biological, nutritional, and therapeutic significance of Moringa oleifera Lam. Phytotherapy research: PTR, 33(11): 2870-2903. https://doi.org/10.1002/ptr.6475.
    12.
  12. Farooq, B., Koul, B., Mahant, D. and Yadav, D. (2021). Phytochemical analyses, antioxidant and anticancer activities of ethanolic leaf extracts of Moringa oleifera Lam. varieties. Plants (Basel, Switzerland), 10(11), 2348. https://doi.org/10.3390/plants10112348.
    13.
  13. Fahey, J.W. (2005). Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees for Life Journal, 1: 1-33.
    14.
  14. Fernandes, Â., Bancessi, A., Pinela, J., Dias, M. I., Liberal, Â., Calhelha, R. C., Ćirić, A., Soković, M., Catarino, L., Ferreira, I. and Barros, L. (2021). Nutritional and phytochemical profiles and biological activities of Moringa oleifera Lam. edible parts from Guinea-Bissau (West Africa). Food chemistry, 341(Pt 1): 128229. https://doi.org/10.1016/j.foodchem.2020.128229.
    15.
  15. Govahi, M., Ghorbani, F., Ranjbar, M., Rahaiee, S. and Azizi, H. (2019). Evaluation of antioxidant and antibacterial activity and determination of phenolic and flavonoid content of aqueous and methanolic extracts of Scutellaria pekinensis. Journal of Ilam University of Medical Sciences, 27(3): 91-100.
    16.
  16. Gull, I., Javed, A., Aslam, M.S., Mushtaq, R. and Athar, M.A. (2016). Use of Moringa oleifera flower pod extract as natural preservative and development of SCAR marker for Its DNA based identification. Bio Med research international, 2016: 7584318. https://doi.org/10.1155/2016/7584318.
    17.
  17. Hendra, R., Ahmad, S., Sukari, A., Shukor, M.Y. and Oskoueian, E. (2011). Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff) boerl. fruit. International Journal of Molecular Sciences, 12(6): 3422-3431.
    18.
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