بررسی اثر تنش شوری بر برخی پاسخهای مورفولوژیکی، بیوشمیایی و آنتیاکسیدانی گیاه دارویی Securigera securidaca L.
محورهای موضوعی : فیزیولوژی محیطیفاطمه میروکیلی 1 , اصغر مصلح آرانی 2 , محمدرضا سرافراز اردکانی 3 , حمید سودائی زاده 4
1 - دانشجوی گروه محیطزیست، دانشکده منابع طبیعی و کویرشناسی، دانشگاه یزد، یزد، ایران.
2 - دانشیار گروه محیطزیست، دانشکده منابع طبیعی و کویرشناسی، دانشگاه یزد، یزد، ایران
3 - استادیار گروه زیستشناسی، دانشکده علوم، دانشگاه یزد، یزد، ایران
4 - دانشیار گروه مدیریت مناطق خشک و بیابانی، دانشکده منابع طبیعی و کویرشناسی، دانشگاه یزد، یزد، ایران
کلید واژه: تنش شوری, آنتوسیانین, آنتیاکسیدانهای آنزیمی, شاخص فعالیت جاروب رادیکال DPPH, عدسالملک, فلاونوئید ,
چکیده مقاله :
در این تحقیق به منظور بررسی اثر تنشهای مختلف شوری بر برخی شاخصهای مورفولوژیکی، بیوشیمیایی و آنتیاکسیدانی گیاه دارویی عدسالملک (Securigera securidaca L.) در قالب طرح کاملاً تصادفی، بذرهای گیاه در مهرماه سال 1394 از موسسه پاکان بذر اصفهان تهیه و مراحل کاشت و رشد گیاه در اتاق رشد در دانشگاه یزد انجام گردید. ارزیابی و مقایسه تغییرات شاخصهای مورفولوژیکی، بیوشیمیایی و عملکرد آنتیاکسیدانی به روش اسپکتروفتومتری اندازهگیری گردید. نتایج نشان داد که با افزایش غلظت شوری برخی شاخصهای مورفولوژیکی بهصورت معنیداری کاهش یافتند. مقدار رنگیزهها در تیمارهای مختلف شوری نسبت به تیمار کنترل تغییر معنیداری نشان نداد. مقدار پرولین، قندهای محلول کل، فلاونوئیدها و آنتوسیانین در بالاترین سطح شوری (12 دسیزیمنس بر متر) نسبت به تیمار کنترل و سایر تیمارهای شوری بهصورت معنیداری افزایش قابل توجهی یافتند. مقدار مالوندیآلدهید در شوری 12 دسیزیمنس بر متر افزایش معنیداری نشان داد در حالی که بیشترین مقدار فعالیت جاروب رادیکالدىفنیلپیکریلهیدرازیل (DPPH) و فعالیت آنزیمهای سوپراکسید دیسموتاز، پراکسیداز و کاتالاز در شوری 8 دسیریمنس بر متر مشاهده شد. یافتههای فوق نشان داد که با توجه به عدم تغییرات معنیدار شاخصهای موفولوژیکی، بیوشیمیایی و آنتیاکسیدانی بویژه در شوریهای پایین (4 دسیزیمنس بر متر)، احتمالاً بتوان رقم عدسالملک مورد مطالعه را بهعنوان یک گونه نیمهحساس (با تحمل متوسط) به شوری پیشنهاد کرد.
In this research in order to study of stress influence(4, 8 and 12 ds/m of NaCl ) on some morphological, biochemical and antioxidantactivity of Securigera securidaca L. based on a randomized complete design with three replications, the seeds of plant were provided from Esfahan Pakan Bazr Institute and were grown in growth chamber located in Yazd University during September, 2014. Biochemical and antioxidant traits were measured spectrophotometrically. Results were showed that some morphological traits significantly decreased when salinity were increased. The pigments content were not significantly affected by different levels of salinity. Proline, total soluble sugar, flavonoid and anthocyanin content significantly increased in highest level of salinity in compared with other salinity and control treatments. Malondialdehyde (MDA) significantly increased in salinity of 12 ds/m while the most significant activity of DPPH scavenging and superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) enzymes were showed in salinity of 8 ds/m. in concluded, due to non-significant changes of morphological, biochemical and antioxidant traits in salinity of 4 ds/m especially, it may be suggested that Securiger securidaca L. is a semi-tolerant genotype under salt stress.
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