بهینه‌سازی، مشخصه‌یابی و فعالیت آنتی‌باکتریایی نانوذرات طلای بیوسنتز شده با استفاده از عصاره آبی گیاه Sambucus ebulus L.

محورهای موضوعی : فیتوشیمی

امید عزیزیان شرمه ¹ , جعفر ولیزاده ² , میثم نوروزی‌فر ³ , علی قاسمی ⁴ , محرم ولیزاده ⁵

1 - کارشناس‌ارشد، گروه شیمی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران
2 - دانشیار، عضو هیأت علمی گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران
3 - استاد، عضو هیأت‌علمی گروه شیمی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران
4 - مربی، عضو هیأت‌علمی گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران
5 - استادیار، عضو هیأت‌علمی گروه تولیدات گیاهی، دانشکده کشاورزی، مجتمع آموزش عالی سراوان، سراوان، ایران

تاریخ دریافت : 1394/11/17 تاریخ پذیرش : 1395/03/23 تاریخ انتشار : 1395/03/01

کلید واژه: آقطی, آنتی‌باکتریایی, بیوسنتز, نانوذرات طلا, Sambucus ebulus L,

چکیده مقاله :

گیاهان به‌عنوان منابع پایدار و دسترس در تهیه نانوذرات زیست سازگار در سال‌های اخیر مورد توجه زیادی قرار گرفته‌اند. هدف از مطالعه حاضر بهینه‌سازی و مشخصه‌یابی و بیوسنتز نانو ذرات طلا با استفاده از عصاره آبی برگ گیاه آقطی (Sambucus ebulus L.) می‌باشد. پس از آماده کردن عصاره، 2 میلی‌لیتر از آن به 4 میلی‌لیتر از نمک طلا (HAuCl4.3H2O) با غلظت 1 میلی‌مولار اضافه شد که عصاره، یون‌های طلا (III) را به اتم‌های طلا در ابعاد نانومتریک کاهش داده و بلافاصله رنگ محلول به رنگ بنفش تغییر داد. به‌منظور دستیابی به نانوذرات طلا با شکل و اندازه یکنواخت، پارامترهای موثر بر سنتز، نظیر pH محیط واکنش، حجم عصاره، غلظت نمک طلا، دما و زمان واکنش مورد مطالعه قرار گرفت و تمامی پارامتر‌ها با استفاده از اسپکتروفتومتری فرابنفش- مرئی بهینه شدند. برای مشخصه یابی نانوذرات از تصویر میکروسکوپ الکترونی عبوری (TEM) و پراش پرتو ایکس (XRD) استفاده شد. در نهایت خواص آنتی باکتریایی نانوذرات بر روی 4 گونه باکتری بیماری‌زا استافیلوکوکوس اورئوس، باسیلوس سوبتیلیس، اشرشیا کلی و سالمونلا اینتریتیدیس) با روش دیسک دیفیوژن مورد بررسی قرار گرفت و به‌صورت قطر‌هاله عدم رشد گزارش شد. نتایج نشان دادند که نانو ذرات طلا کروی بوده و علاوه بر اینکه حداکثر جذب را در 534 نانومتر دارند، با میانگین اندازه بین 17-11 نانومتر از فعالیت آنتی‌باکتریایی نسبتاً خوبی نیز علیه باکتری‌های برخوردارند.

چکیده انگلیسی:

Plants as stable and available sources to preparation of the biocompatible nano particles that have received much attention in recent years. The aim of this study were optimization and characterization of biosynthesis of gold nano particles (Au NPs) using leaf aqueous extract of Sambucus ebulus L. After preparing the extract, 2 ml of it, was added to 4 ml of HAuCl4.3H2O with concentration 1 mM, which reduced Au (III) ions to Au NPs, and quickly the color of solution changed to violet. In order to achieve Au NPs with a uniform shape and size, parameters affecting on synthesis such as: pH of the reaction, volume of plant extract, concentration of HAuCl4.3H2O solution, temperature and time of reaction were studied and optimized. All  parameters were optimized using UV-Vis spectrophotometry. X-ray diffraction (XRD) and transmission electron microscopy (TEM) were also employed to find characterization of the nanoparticles. Finally, the antibacterial properties of nanoparticles on 4 species of pathogenic bacteria such as: Staphylococcus aureus, Bacillus subtilis, E. coli and Salmonella enteritidis was evaluated by disc diffusion method and was reported as the diameter of inhibition zone. The results showed that the Au NPs showed a maximum absorbance at 534 nm. It was found that biosynthesized of Au NPs have spherical shape with a size between 11-17 nm and the gold nanoparticles have relatively good antibacterial activity against some bacteria.  

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