مقایسه مورفولوژیکی، فیزیکوشیمیایی و آنتی‌اکسیدانی میوه گیاه دارویی خرمندی (Diospyros lotus L.) در سه منطقه از جنگل‌های هیرکانی
محورهای موضوعی : اکولوژی محیطی
کلید واژه: آنتوسیانین, آنتی‌اکسیدان, جنگل هیرکانی, خرمالوی وحشی, خرمندی, فلاونوئید, فنول,
چکیده مقاله :
جنگل‌های هیرکانی اکوسیستم منحصربه‌فرد و باستانی در امتداد سواحل جنوبی دریای خزر هستند. بررسی تنوع گیاهان دارویی موجود در این جنگل‌ها، ازجمله خرمندی یا خرمالوی وحشی (Diospyros lotus L.)، کمک زیادی به حفظ ذخایر ژنتیکی آنها می‌کند. هدف از این پژوهش ارزیابی میوه خرمندی بومی جنگل‌های هیرکانی از سه منطقه حومه گرگان و علی‌آباد در استان گلستان و حومه بهشهر در استان مازندران ازنظر صفات مورفولوژیکی، فیزیکوشیمیایی و آنتی‌اکسیدانی بود. بدین منظور، نمونه‌های میوه در آذر 1400 در زمان برداشت تجاری-بومی جمع‌آوری و مورد ارزیابی قرار گرفتند. بر اساس نتایج به‌دست‌آمده، بیشترین وزن، قطر و حجم میوه به منطقه بهشهر و کمترین وزن، طول، قطر و حجم میوه به منطقه گرگان اختصاص یافت. ازنظر شکل ظاهری میوه، نمونه‌های منطقه بهشهر نسبت به گرگان و علی‌آباد کشیده‌تر بودند. مقایسه صفات کیفی میوه‌ها نشان داد که بیشترین میزان ویتامین ث و اسیدیته میوه به منطقه بهشهر، بیشترین pH و مواد جامد محلول به منطقه گرگان، بیشترین میزان فنول و فلاونوئید کل به مناطق علی‌آباد و بهشهر و بیشترین میزان آنتوسیانین کل و ظرفیت آنتی‌اکسیدانی به منطقه گرگان اختصاص یافت. بررسی همبستگی بین صفات نشان داد که وزن میوه با اسیدیته همبستگی مثبت معنی‌دار داشت، ولی با pH و ظرفیت آنتی‌اکسیدانی همبستگی منفی معنی‌دار نشان داد. اسیدیته با ظرفیت آنتی‌اکسیدانی و آنتوسیانین کل همبستگی منفی معنی‌دار داشت. در مقابل، pH و مواد جامد محلول با آنتوسیانین کل همبستگی مثبت معنی‌دار نشان دادند. به‌طورکلی، تنوع زیادی بین میوه دارویی خرمندی وحشی در سه ناحیه از جنگل‌های هیرکانی ازنظر خصوصیات مورفولوژیکی، فیزیکوشیمیایی و ظرفیت آنتی‌اکسیدانی مشاهده شد.
The Hyrcanian forests, located along the southern shores of the Caspian Sea, are a unique and ancient ecosystem. To preserve the genetic reserves of these forests, it is essential to examine the diversity of their medicinal plants , including the date-plum or wild persimmon (Diospyros lotus L.). The aim of this research was to evaluate the native date-plum fruit harvested from three regions of the Hyrcanian forests including Gorgan, Aliabad, and Behshahr in terms of morphological, physicochemical, and antioxidant traits. to do so, fruit samples were collected and evaluated in November 2021 during commercial-local harvest. The obtained results showed that the highest fruit weight, diameter, and volume were recorded in samples collected from Behshahr, while the lowest weight, length, diameter, and fruit volume were observed in fruits belonging to Gorgan. This research indicated that fruits collected from Behshahr were more elongated compared to those collected from Gorgan and Aliabad. The comparison of qualitative traits confirmed that the highest acidity and vitamin C content were observed in Behshahr and the highest pH and total soluble solids were recorded in the fruit samples of Gorgan. The maximum total phenol and flavonoid were related to Aliabad and Behshahr. Finally, the highest total anthocyanin and antioxidant capacity were observed in Gorgan. Furthermore, this study found that fruit weight had a significant positive correlation with acidity while it showed a significant negative correlation with pH and antioxidant capacity. Also, fruit acidity had a significant negative correlation with antioxidant capacity and total anthocyanin. In contrast, pH and total soluble solids showed a significant positive correlation with total anthocyanin. In general, a great diversity was observed among the medicinal fruit of the wild date-plum collected from the three investigated regions in Hyrcanian forests with regard to the morphological, physicochemical, and antioxidant activity.
Ajami, M., Seifi, E., Varasteh, F., and Atashi, S. 2023. Evaluation of the morphological, phytochemical and antioxidant capacity of (Punica granatum L.) Toosefid cultivar from Gorgan in comparison with two commercial cultivars. Eco-phytochemical Journal of Medicinal Plants. 10(4): 14-26.
Akhani, H., Djamali, M., Ghorbanalizadeh, A., and Ramezani, E. 2010. Plant biodiversity of Hyrcanian relict forests, Iran: an overview of the flora, vegetation, palaeoecology and conservation. Pakistan Journal of Botany, 42(1), 231-258.
Amanzadeh, B., Pourmajidian, M. R., Sagheb-Talebi, K., and Hojjati, S. M. 2015. Impact of canopy gap size on plant species diversity and composition in mixed stands, case study: Reserve area, District No. 3 Asalem Forests. Forest and Wood Products, 68(2), 287-301.
Anatov, D., and Mallaliev, M. 2022. Intrapopulation variability of fruit quantitative traits Diospyros lotus L. in the Inner Mountain Dagestan. In BIO Web of Conferences (Vol. 43, p. 01013). EDP Sciences.
Azarhoosh, K.H., Sharifi, A. and Estiri, S.H. 2017. Effect of sugar replacement with date plum (Diospyros lotus) syrup on antioxidant activity, phenolic compounds and sensory properties of functional cookies. Journal of Food Science and Technology, 1(31): 115-123.
Baiano, A., Gambacorta, G., Terracone, C., Previtali, M. A., Lamacchia, C., and La Notte, E. 2009. Changes in phenolic content and antioxidant activity of Italian extra‐virgin olive oils during storage. Journal of food science, 74(2), C177-C183.
Cho, M.J., Howard, L.R., Prior, R.L. and Clark, J.R. 2005. Flavonoid glycosides and antioxidant capacity of various blackberry, blue¬berry and red grape genotypes determined by high-performance liquid chromatograph/mass spectrometry. Journal of the Science of Food and Agriculture. 84: 1771–1782.
Dujmović Purgar, D., Duralija, B., Voća, S., Vokurka, A. and Ercisli, S. 2012. A comparison of fruit chemical characteristics of two wild grown Rubus species from different locations of Croatia. Molecules. 17(9): 10390-10398.
Esmaeili, S.Z., Dianati, M., Cherati, A. and Moradi, H. 2012. Evaluation of some morphologic and biochemical characters of wild black berry in mountain foot and plain. In: Proceedings of National congress of medicinal plants, 20-21 Nov., Islamic Azad University, Amol, Iran, 1-5.
Fawole, O.A. and Opara, U.L. 2013. Changes in physical properties, chemical and elemental composition and antioxidant capacity of pomegranate (cv. Ruby) fruit at five maturity stages. Scientia Horticulturae, 150: 37-46.
Gao, H., Cheng, N., Zhou, J., Wang, B., Deng, J., and Cao, W. 2014. Antioxidant activities and phenolic compounds of date plum persimmon (Diospyros lotus L.) fruits. Journal of food science and technology, 51, 950-956.
Giusti, M.M. and Wrolstad, R.E. 2001. Characterization and measurement of anthocyanins by UV‐visible spectroscopy. Current Protocols in Food Analytical Chemistry, 1: F1.2.1-F1.2.13.
Guevara-Terán, M., Gonzalez-Paramás, A. M., Beltrán-Noboa, A., Giampieri, F., Battino, M., Tejera, E., and Alvarez-Suarez, J. M. 2022a. Influence of altitude on the physicochemical composition and antioxidant capacity of strawberry: A preliminary systematic review and meta-analysis. Phytochemistry Reviews, 1-18.
Guevara-Terán, M., Padilla-Arias, K., Beltrán-Novoa, A., González-Paramás, A. M., Giampieri, F., Battino, M., ... and Alvarez-Suarez, J. M. 2022b. Influence of altitudes and development stages on the chemical composition, antioxidant, and antimicrobial capacity of the wild andean blueberry (Vaccinium floribundum Kunth). Molecules, 27(21), 7525.
Hassan, A. M., Zannou, O., Pashazadeh, H., Ali Redha, A., and Koca, I. 2022. Drying date plum (Diospyros lotus L.) fruit: Assessing rehydration properties, antioxidant activity, and phenolic compounds. Journal of Food Science, 87(10), 4394-4415.
Heidari Masteali, S. 2021. Evaluation and comparison of biodiversity indexes of tree species in hyrcanian forests (Case study: Kheyroud, Ramsar and Neka Forests). Journal of Plant Research (Iranian Journal of Biology), 34(2), 315-326.
Karimian, A., Taheri Abkenar, K. and Torkaman, J. 2015. Variations in leaf and fruit morphological traits of sweet chestnut (Castanea sativa) in Hyrcanian Forests, Iran. International Journal of Plant Science and Ecology, 1(4): 155-161.
Kashyap, G. and Gautam, M. 2012. Analysis of vitamin C in commercial and natural substances by iodometric titration found in Nimar and Malwa regions. Journal of Scientific Research in Pharmacy, 1(2): 77-78.
Khademi, O., Erfani-Moghadam, J., and Rasouli, M. 2022. Variation of some Diospyros genotypes in Iran based on pomological characteristics. Journal of Horticulture and Postharvest Research, 5(4), 323-336.
Koekemoer, T. C., Swanepoel, B., Rashed, K., and Van De Venter, M. 2021. Diospyros lotus L. fruit: A potential antidiabetic functional food targeting intestinal starch hydrolysis. Egyptian Journal of Chemistry, 64(5), 2445-2451.
Kolbadinejad, M., and Najafian, L. 2020. Identification of chemical compounds and evaluation of antioxidant and antimicrobial properties of date plum (Diospyros Lotus) syrup. Journal of food science and technology (Iran), 17(100), 151-163.
Moradkhani, S. 2022. Phytochemical and antioxidant activity of some of Morus alba L. Var. Nigra genotypes in West and East Azerbaijan province. Eco-phytochemical Journal of Medicinal Plants, 10(3): 56-68.
Qin, L., Xie, H., Xiang, N., Wang, M., Han, S., Pan, M. and Zhang, W. 2022. Dynamic changes in anthocyanin accumulation and cellular antioxidant activities in two varieties of grape berries during fruit maturation under different climates. Molecules, 27(2), 384.
Rahimkhani, R., Varasteh, F. and Seifi, E. 2016. Evaluation of genetic diversity in some loquat genotypes based on pomological characteristics in Golestan province. Plant Production, 23(1), 157-177.
Rauf, A., Abu-Izneid, T., Rashid, U., Alhumaydhi, F.A., Bawazeer, S., Khalil, A.A., and Ntsefong, G.N. 2020. Anti-inflammatory, antibacterial, toxicological profile, and in silico studies of dimeric naphthoquinones from Diospyros lotus. BioMed Research International, Article ID 7942549, 1-10.
Reyes‐Carmona, J., Yousef, G.G., Martínez‐Peniche, R.A. and Lila, M.A. 2005. Antioxidant capacity of fruit extracts of blackberry (Rubus sp.) produced in different climatic regions. Journal of food science. 70(7): s497-s503.
Rezaei Aderyani, F., Rezaei, A., and Sharafi, Y. 2017. Investigation of improving salinity stress damages in Diospyros lotus seedlings by putrescine and chitosan. Journal of Crops Improvement, 19(3), 671-686.
Sadeghinik, M., and Nejad Ebrahimi, S. 2022. Comparison of physicochemical properties and fatty acid profile of seed oil from Citrus aurantium L. extraction in different extraction methods. Eco-phytochemical Journal of Medicinal Plants, 10(2), 1-16.
Samakoush, G.T., Tabande, S.A., and Hakimi, M.M.H. 2017. Investigation of between and within population variation in Diospyros Lotus L. using leaf characteristics analysis (case study: eastern Bandpey of Babol). Journal of Plant Ecosystem Conservation, 4(9):165-180.
Selcuk, N. and Erkan, M. 2014. Changes in antioxidant activity and postharvest quality of sweet pomegranates cv. Hicrannar under modified atmosphere packaging. Postharvest Biology and Technology, 92: 29-3.
Singleton, V. and Rossi, J.A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3): 144-158.
Sun, T. and Ho, C.T. 2005. Antioxidant activity of buck wheat extracts. Food Chemistry, 90: 743-749.
Tromp, J., and Wertheim, S.J. 2005. Fruit growth and development. Fundamentals of temperate zone tree fruit production, 240-266.
Uddin, G., Rauf, A., Siddiqui, B.S., and Shah, S.Q. 2011. Preliminary comparative phytochemical screening of Diospyros lotus Stewart. Middle-East Journal of Scientific Research, 10(1), 78-81.
Yang, Y., Yang, T., and Jing, Z. 2015. Genetic diversity and taxonomic studies of date plum (Diospyros lotus L.) using morphological traits and SCoT markers. Biochemical Systematics and Ecology, 61, 253-259.
Yildirim, N., Ercişli, S., Ağar, G., Orhan, E., and Hizarci, Y. 2010. Genetic variation among date plum (Diospyros lotus) genotypes in Turkey. Genetics and Molecular Research 9 (2): 981-986.
Yue, X., Yang, H., Wang, T., Dong, S., Sun, Z., Wang, Y., ... and Peng, W. 2020. Molecules and medical function of Diospyros lotus L. Thermal science, 24(3 Part A), 1705-1712.