ترکیبات فیتوشیمیایی، فعاليت آنتی‌اکسیدانی و اثرات ضدسرطاني عصاره ریشه و جوانه کینوا (Chenopodium quinoa) بر رده AGS سرطان معده
محورهای موضوعی : فیتوشیمی
فاطمه شیرعلی
1
,
سیّد ابراهیم سیفتی
2
1 - گروه مدیریت مناطق خشک و بیابانی، دانشکده منابع طبیعی و کویرشناسی، دانشگاه یزد، یزد، ایران
2 - مدیریت مناطق خشک و بیابانی ، منابع طبیعی و کویرشناسی ، دولتی یزد ، ایران
کلید واژه: اثرات سیتوتوکسیکی, آزمون سمیت MTT, تیتیکاکا, فلاونوئید, ساپونین,
چکیده مقاله :
به منظور بررسی ترکیبات فیتوشیمیایی، فعالیت آنتی‌اکسیدانی و اثر ضدسرطانی ریشه و جوانه ارقام مولتی‌هد بالک، بلک و تیتیکاکا بر رده سلولی آدنوکارسینوم (AGS) سرطان معده، ارقام در قالب طرح بلوک¬های کامل تصادفی با 4 تکرار در مزرعه مناطق خشك و نيمه‌خشک ایران در دانشگاه یزد کشت شدند. نمونه‌های ریشه، قبل از پرشدن دانه و از عمق 30 سانتی‌متری خاک برداشت شد. از هر رقم، پس از کامل شدن مرحله رشد زایشی و پر شدن دانه، 100 گرم بذر به طور تصادفی جهت تولید جوانه بدون این که پوست‌گیری شود، برداشت گردید. بیشترین میزان ساپونین بر حسب میلی‌گرم بر گرم وزن خشک نمونه، در ریشه بلک (24/4) و سپس ریشه تیتیکاکا (7/1) و کمترین میزان آن (6/0) در جوانه بلک مشاهده شد. محتوای فیتوشیمیایی و آنتی‌اکسیدانی عصاره هیدرواتانولی (80:20) به روش سوکسله ریشه و جوانه هر سه رقم، در غلظت‌هاي 100، 250، 500 و 1000 ميلي‌گرم بر ميليليتر بررسی گردید. بر این اساس، به ترتیب عصاره جوانه ارقام بلک و تیتیکاکا در غلظت 1000 میلی‌گرم بر میلی‌لیتر از نظر محتوای كل تركيبات فنولی (68/172 میلی‌گرم گالیک اسید به گرم ماده خشک)، فلاونوئيدي (17/128 میلی‌گرم کوئرستین به گرم ماده خشک) و ظرفيت آنتی‌اکسیدانی، نسبت به نمونه‌های متناظر در رقم مولتی‌هد بالک و همچنین نمونه ریشه هر سه رقم، برتری معنی‌داری داشتند. کمترین و بیشترین DPPH IC50 به ترتیب در عصاره جوانه بلک (11/1 میلی‌گرم بر میلی‌لیتر) و ریشه مولتی‌هد بالک (15/3) مشاهده شد. كمترين درصد زنده‌ماني سلول‌های رده AGS به ترتيب در غلظت 1000 ميلي‌گرم بر ميليليتر عصاره جوانه بلك (46/33) و تيتيكاكا (11/36) در زمان 72 ساعت بود. نتایج این مطالعه پس از بررسی خصوصیات فیتوشیمیایی و آنتی‌اکسیدانی جوانه در مقایسه با بذر ارقام مورد مطالعه در دو حالت پوست‌گیری شده و دارای پوست، می‌تواند منجر به توسعه و تسهیل دسترسی بیماران مبتلا به سرطان به این گیاه دارویی شود.
This study aimed to investigate the phytochemical composition, antioxidant activity, and anticancer effects of root and sprout samples from Multi-hued Bulk, Black, and Titicaca Quinoa varieties (Chenopodium quinoa willd.) on Human Gastric Adenocarcinoma Cell Line (AGS). These varieties were cultivated using a complete randomized block design with four replications in arid and semi-arid regions of Iran (research field of Yazd University). Root samples were harvested before seed filling from a soil depth of 30 cm. Once the seed germination and filling stages were complete, 100 g of seeds from each variety were randomly selected without hulling to produce sprouts. The highest saponin contents (mg/g dry weight) were observed in the Black root (24.4), followed by the Titicaca root (7.1). The lowest saponin content (0.6) was found in the Black sprout. The phytochemical composition, and antioxidant activity of the hydroethanolic (80:20) extract (obtained using the Soxhlet method), at 100, 250, 500, and 1000 mg/mL were evaluated for root and sprout samples of all three varieties. Based on the results, the Black and Titicaca sprout extract had significantly higher total phenolic (172.68 mgGAE/gDW) and flavonoid (128.17 mgQ/gDW) contents, as well as antioxidant capacity at 1000 mg/mL compared to the corresponding samples from the Multi-hued Bulk variety and the root samples of all three varieties. The lowest and highest DPPH IC50 value was observed in the Black sprout and Multi-hued Bulk root extracts (1.11 and 3.15 mg/mL, respictively). The average cell viability of AGS was observed in the Black sprout extract (33.46) and Titicaca (36.11) varieties at 1000 mg/mL, 72 hours after the treatment, respectively. Findings suggested that the phytochemical and antioxidant properties of Quinoa sprouts, compared to hulled and unhulled seeds of the investigated varieties, could contribute to the development and accessibility of this medicinal plant for patients with cancer.
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