جداسازی و شناسایی انتروکوکوس فکالیس دارای ژن مقاوم به ونکومایسین از رودخانه قلعه رودخان، ایران در سال 1401
محورهای موضوعی : Biotechnological Journal of Environmental Microorganisms
خسرو عیسی زاده
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اوریا رضاپور
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محمد فائزی قاسمی
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1 - گروه میکروبیولوژی ، دانشکده علوم پایه، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان ، ایران
2 - گروه میکروبیولوژی ، دانشکده علوم پایه ، واحد لاهیجان ، دانشگاه آزاد اسلامی لاهیجان ، ایران
3 - گروه میکروبیولوژی ، دانشکده علوم پایه ، واحد لاهیجان، دانشگاه آزاد اسلامی ،لاهیجان ، ایران
کلید واژه: انتروکوکوس فکالیس, مقاومت آنتی بیوتیکی, انتروکوکوس فکالیس مقاوم به ونکومایسین, آلودگی آب,
چکیده مقاله :
انتروکوک های مقاوم به وانکومایسین (VRE) باکتری های بسیار نگران کننده ای هستند که در لیست اولویت جهانی باکتری های مقاوم به آنتی بیوتیک توسط سازمان بهداشت جهانی فهرست شده اند. مقاومت آنها در برابر آنتی بیوتیک ها به دلیل فشارهای محیطی، سلامت عمومی را تهدید می کند. این باکتری می تواند در شرایط سخت و محیط های کم مواد مغذی زنده بماند و رشد کند و به راحتی از طریق مدفوع - دهانی منتقل می شود. انتروکوک های مقاوم به وانکومایسین منتقله از آب یک مشکل زیست محیطی و بهداشتی است. هدف از مطالعه حاضر بررسی شیوع جدایه های انتروکوکوس مقاوم به وانکومایسین و الگوی آنتی بیوتیکی آنها در نمونه های محیطی رودخانه قلعه رودخان در استان گیلان بود. نمونه‌های آب از رودخانه جمع‌آوری و برای وجود انتروکوک‌ها با استفاده ازمحیط کشت بایل اسکولین آزید آگار آزمایش شدند. پس از جمع‌آوری، نمونه‌ها با استفاده از آزمایش‌های بیوشیمیایی و تکنیک‌های مولکولی با پرایمرهای اختصاصی برای ddl شناسایی شدند. الگوی مقاومت آنتی بیوتیکی جدایه ها با استفاده از تست آنتی بیوگرام تعیین شد. علاوه بر این، شناسایی مولکولی ایزوله‌های مقاوم به وانکومایسین با استفاده از واکنش زنجیره‌ای پلیمراز (PCR) با پرایمرهای اختصاصی برای ژن‌های vanA و vanB انجام شد. از 263 نمونه، انتروکوک‌ها در 154 نمونه (69%) پس از تایید ژن ddlE یافت شدند. تجزیه و تحلیل از بین این جدایه ها، 130 جدایه (84%) به عنوان VRE شناسایی شدند. نتایج نشان داد که تقریباً همه جدایه‌ها دارای ژن‌های مقاوم به وانکومایسین بودند که ۸۹ ایزوله (۴۶/۶۸ درصد) حامل vanA و ۱۱۱ ایزوله (۳۸/۸۵ درصد) حامل vanB بودند. این مطالعه نشان می دهد که بسیاری از ایزوله های انتروکوک به دست آمده به استرپتومایسین، کانامایسین، جنتامایسین و وانکومایسین مقاوم بودند. همچنین مشخص شد که VRE حامل ژن‌های vanA و vanB در رودخانه قلعه رودخان وجود دارد و منابع VRE در سراسر حوضه رودخانه پخش شده‌اند. شیوع بالای و میزان مقاومت آنتی‌بیوتیکی سویه‌های انتروکوک مقاوم به وانکومایسین در منابع آبی استان گیلان نگران‌کننده و نیازمند توجه است.
Vancomycin-resistant enterococci (VRE) are highly concerning bacteria listed on the Global Priority List of Antibiotic-Resistant Bacteria by the World Health Organization. Their resistance to antibiotics poses a significant threat to public health due to environmental pressures. This bacterium can survive and grow in harsh conditions and low-nutrient environments, and can easily be transmitted via the fecal-oral route. Water-borne VRE is an environmental and health problem. The aim of the present study was to investigate the prevalence of VRE isolates and their antibiotic patterns in environmental samples from the Ghale Rudkhan River in Guilan Province, Iran. The water samples were collected from the river and tested for the presence of Enterococci using bile esculin azide agar. After collection, the samples were identified using biochemical tests and molecular techniques with specific primers for the ddlE. Antibiotic resistance patterns of the isolates were determined using an antibiogram test. Furthermore, molecular identification of the vancomycin-resistant isolates was conducted using polymerase chain reaction (PCR) with specific primers for the vanA and vanB genes.Out of 263 samples, Enterococci were found in 154 samples (69%) after a confirmatory ddlE gene analysis. Among these isolates, 130 (84%) were identified as VRE. The results indicated that nearly all of the isolates had vancomycin-resistant genes, with 89 isolates (68.46%) carrying vanA and 111 isolates (85.38%) carrying vanB. This study reveals that many of the enterococcal isolates obtained were resistant to streptomycin, kanamycin, gentamicin, and vancomycin. It also found that VRE carrying vanA and vanB genes are present in the Ghale Rudkhan River and that the sources of VRE are spread throughout the river basin. The high prevalence and antibiotic resistance rate of VRE strains of E. faecalis in water resources in Guilan province are concerning and require attention.
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