Antimicrobial and Anti-biofilm Effect of Samarium oxide Nanoparticles on Pseudomonas aeruginosa Isolated from Hospital Wards

Subject Areas : Biotechnological Journal of Environmental Microbiology

Hossein Zahmatkesh ¹ , Mirsasan Mirpour ^{2 *} , Hojjatolah Zamani ³ , Behnam Rasti ⁴

1 - گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد لاهیجان، لاهیجان، ایران
2 - گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد لاهیجان، لاهیجان، ایران
3 - گروه زیست شناسی، دانشگاه گیلان، رشت، ایران
4 - گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد لاهیجان، لاهیجان، ایران

Received: 2022-10-06 Accepted : 2022-11-19 Published : 2022-11-20

Keywords: Nanoparticles, Samarium, Pseudomonas aeruginosa, Hospital environment, Biofilm,

Abstract :

The development of drug resistance, treatment ineffectiveness, and the occurrence of Pseudomonas aeruginosa
infections are primarily attributed to biofilm formation and quorum sensing (QS)-dependent virulence
factors. This study aimed to synthesize samarium oxide nanoparticles (Sm2O3NPs) using curcumin and evaluate
their antimicrobial effects against P. aeruginosa isolated from different hospital wards. Based on TEM,
XRD, and EDX analysis, the synthesized nanoparticles exhibited a cubic structure with an average size of
32.61 nm and a pure crystalline phase. The Sm2O3NPs exhibited notable antibacterial efficacy against P. aeruginosa
strains. Based on the CV staining assay, sub-MIC of Sm2O3NPs inhibited the biofilm formation of P.
aeruginosa strains by 16-69%. Additionally, the levels of pyocyanin in the NPs-treatment groups decreased
by 29-58% compared to the control group. This research confirms the significant antibacterial potential of
Sm2O3NPs, implying their potential utilization as an effective antibacterial agent against P. aeruginosa strains
isolated from various hospital wards, after further characterization.