Antimicrobial activity of Ethanolic extracts of (Tragopogon collinus L. and Allium eriophyllum L.) on some selected environmental and pathogenic bacteria

Subject Areas : Biotechnological Journal of Environmental Microbiology

Ehsan Abolfathi ^{1 *} , Nour Amirmozafari ² , Ezaat Ghaemi ³

1 - گروه میکروبیولوژی و ایمونولوژی ، دانشکده دامپزشکی ، دانشگاه تهران ، تهران، ایران
2 - گروه میکروبیولوژی ، دانشکده پزشکی ، دانشگاه علوم پزشکی ایران ، تهران ، ایران
3 - گروه میکروبیولوژی پزشکی ، دانشگاه علوم پزشکی گرگان ، گرگان ، ایران

Received: 2022-08-29 Accepted : 2022-09-24 Published : 2022-03-01

Keywords: Antibacterial activity, Ethanolic extract, Tragopogon collinus L., Allium eriophyllum L,

Abstract :

Due to the increase in the incidence of infectious diseases and the prevalence of antibiotic resistance in bacteria
in different environments, there is an urgent need to discover new antimicrobial compounds. The present study
aimed to determine the antibacterial activity of the ethanolic extracts of the edible plants Tragopogon Collinus
L. and Allium eriophyllum L. against some environmental and pathogenic bacteria such as Staphylococcus
aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, and Enterococcus faecalis. The
extraction of medicinal plants was conducted using ethanol and the percolation method. The well diffusion
method was used to determine the antimicrobial effects of the extracts, and the minimum inhibitory concentration
(MIC) of microbial growth. The ethanol extract of T. collinus showed an inhibitory effect on all bacteria
in 250 μg/ml concentration, and the greatest effect was observed on S. aureus and E. faecalis, with 20 and 18
mm diameter of zone, respectively. In the 125 μg/ml concentration, with a 21 mm diameter of zone, it had the
highest antimicrobial effect on K. pneumoniae, compared with other bacteria. Regarding the ethanolic extract
of A. eriophyllum L., the highest antibacterial effect at 250 μg/ml concentration was 20 and 22 mm zone diameters.
The effect of this plant extract on P. aeruginosa was lower than its effect on other bacteria. The MIC
of the extract of Tragopogon collinus L.was found at a 5.75 μ/ml concentration on S. aureus and E. faecalis.
The MIC of the ethanolic extract of A. eriophyllum L. was found at 11.5 μ/ml concentration on S. aureus, P.
aeruginosa, and K. pneumoniae. The results of this study indicate that T. collinus and A. eriophyllum have
antibacterial properties.