Isolation and Characterization of the Symbiotic- Pathogenic Bacteria isolated from Trifolium resupinatum Plant

Subject Areas : Biotechnological Journal of Environmental Microbiology

Nazanin Moazenpour ¹ , Monir Doudi ^{2 *} , Ladan Rahimzadeh Torabi ³ , Mohammad Hossein Pazandeh ⁴

1 - گروه میکروبیولوژی ، واحد فلاورجان، دانشگاه آزاد اسلامی ، اصفهان ، ایران
2 - گروه میکروبیولوژی ، واحد فلاورجان ، دانشگاه آزاد اسلامی ، اصفهان ، ایران
3 - گروه میکروبیولوژی ، واحد فلاورجان ، دانشگاه آزاد اسلامی ، اصفهان ، ایران
4 - گروه داروسازی و سم شناسی ، دانشکده داروسازی ، دانشگاه علوم پزشکی همدان ، همدان ، ایران

Received: 2022-12-09 Accepted : 2022-12-25 Published : 2022-12-27

Keywords: Symbiotic-Pathogenic Bacteria, Trifolium resupinatum, Erwinia chrysanthemi, Pectobacterium carotorum, and Klebsiella oxytoca,

Abstract :

Plant diseases have a significant impact on plants and their crop yields, causing extensive epidemics and recurrent
damages that result in profound negative effects. Bacteria such as Erwinia, Pectobacterium and Klebsiella
have a very wide host range and can play a pathogenic role for a large number of ornamental and agricultural
plants or even establish a symbiotic relationship with the plant. This group of bacteria that cause all kinds of
plant diseases are able to affect seed tubers and soil microbial community. The objective of this investigation
was to identify and classify the distinct symbiotic pathogens associated with Trifolium resupinatum plants obtained
from the Shahreza region located in the southern part of Isfahan, Iran. In this investigation, T. resupinatum
specimens harboring nodular root structures were initially identified and subsequently retrieved from various
locations in the southern region of Isfahan (Shahreza), before being transported to the laboratory. The Yeast
Mannitol Agar (YMA) medium underwent a cultivation procedure, subsequent to which the bacterial samples
were subjected to molecular identification utilizing morphological and biochemical tests. Additionally, the
colony-PCR technique was employed to achieve definitive identification. This study examined the molecular
features of three distinct species namely Erwinia chrysanthemi, Pectobacterium carotorum, and Klebsiella oxytoca.
It was revealed that the former two species exhibited a symbiotic pathogenic relationship with the T. resupinatum
plant, while the latter species posed a threat to human health as a pathogen. The study results revealed
that the root nodes of leguminous plants displayed the coexistence of beneficial symbiotic rhizobium species
that are known for their capacity to enhance plant growth in both dicotyledonous and monocotyledonous plants.