شاخص‌های بیوشیمیایی و ایمنی همولنف میگوی ببری سبز (Penaeus semisulcatus) در سیستم آبزی‌پروری بیوفلوک: تحت تاثیر سطوح مختلف غذایی
محورهای موضوعی : فصلنامه زیست شناسی جانوری
1 - گروه علوم و مهندسی شیلات، دانشکده منابع طبیعی، دانشگاه جیرفت، جیرفت، کرمان، ایران
کلید واژه: میگوی ببری سبز, بیوفلوک, ایمنی, همولنف,
چکیده مقاله :
تأثیر سطوح مختلف غذایی بر شاخص‌های بیوشیمیایی و ایمنی میگوی ببری سبز در سیستم آبزی‌پروری بیوفلوک موردبررسی قرار گرفت. آزمایش به مدت 45 روز با میگوهای با میانگین وزن 85/2 گرم انجام شد. مخازن پرورش با 150 ليتر آب تصفيه شده با فيلتر شني پر شدند و سپس تعداد 53 قطعه میگو در هر مخزن ذخيرهسازي شد. 7 گروه آزمايشي براي تحقيق حاضر در نظر گرفته شد که شامل سه گروه کنترل با سطوح مختلف غذایی بر حسب درصد وزن بدن، 6 درصد (CW6)، 4 درصد (CW4) و 2 درصد (CW2) و چهار تیمار بیوفلوک 6 درصد (BFT6)، 4 درصد (BFT4)، 2 درصد (BFT2) و 0 درصد (BFT0) بود. نتایج نشان داد، بالاترین میزان تری‌گلیسرید (0/175 میلیگرم/دسیلیتر) و کلسترول (142 میلیگرم/دسیلیتر) در تیمار CW2 بدست آمد. بالاترین میزان گلوکز (47 میلیگرم/دسیلیتر) در تیمار BFT0 و کمترین مقدار آن 66/35 و 35/36 به تریتیب در تیمارهای BFT6 و BFT4 مشاهده شد که اختلاف معنی‌داری با سایر تیمارها نشان داد (05/0 > p). بالاترین مقدار فعالیت لیزوزیم در تیمارهای BFT6 و BFT4 به ترتیب 6/24 و 3/24 واحد/میلیلیتر/دقیقه و کمترین مقدار آن در تیمار BFT0 (15 واحد/میلیلیتر/دقیقه) بدست آمد که اختلاف معنی‌داری با سایر تیمارها نشان داد (05/0> p). بالاترین میزان فعالیت فنول اکسیداز در در تیمارهای BFT6 (74/0 واحد/میلیلیتر) و BFT4 (75/0 واحد/میلیلیتر) بالاترین بود و کمترین مقدار آن در تیمار BFT0 (u/ml 37/0 (واحد/میلیلیتر) بدست آمد. نتایج نشان داد، سطوح مختلف غذایی بر فعالیت‌های بیوشیمیایی و ایمنی همولنف میگوی ببری سبز تاثیر میگذارد. تغذیه با سطوح غذایی 6 و 4 درصد در سیستم بیوفلوک منجر به بهبود فعالیت‌های لیزوزیم، ایمنوگلوبولین کل و فنول اکسیداز در میگوی ببری سبز می‌شود.
The effect of different feeding levels on the biochemical indicators and immunity of green tiger shrimp in a biofloc aquaculture system was investigated. The experiment was conducted for 45 days with shrimps with an average weight of 2.85 g. Rearing tanks was filled with 150 liters of filtered water with a sand filter, and then 53 individual shrimps were stored in each tank. 7 experimental groups were considered for this research, which included three control groups with different feeding levels in terms of body weight, 6% (CW6), 4% (CW4) and 2% of body weight (CW2), and four biofloc treatments of 6% (BFT6), 4% (BFT4), 2% (BFT2) and 0% (BFT0). The results showed that the highest levels of triglyceride (175.0 mg/dl) and cholesterol (142 mg/dl) were observed in the CW2 treatment. The highest amount of glucose (47 mg/dl) was obtained in BFT0 treatment and the lowest amount was 35.66 and 36.35 in BFT6 and BFT4 treatments, which showed a significant difference with other treatments (p < 0.05). The highest value of lysozyme activity was obtained in BFT6 and BFT4 treatments (24.6 and 24.3 u/ml/min), respectively, and the lowest value was obtained in BFT0 treatment (15 u/ml/min), which showed a significant difference with other treatments (p > 0.05). The highest amount of phenol oxidase activity was the highest in BFT6 (0.74 u/ml) and BFT4 (0.75 u/ml) treatments, and the lowest value was obtained in BFT0 treatment (0.37 u/ml). In general, the results showed that different feed levels affect the biochemical activities and immunity of green tiger shrimp hemolymph. Feeding with food levels of 6 and 4% in biofloc system leads to improvement of lysozyme, total immunoglobulin and phenol oxidase activities in green tiger shrimp.
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