اثر عصاره جینسنگ (Panax ginseng) بر تغییرات هیستومورفومتریک مخ و مخچه در نوزادان چهارده روزه موش صحرایی مادران دیابتی
محورهای موضوعی : فصلنامه زیست شناسی جانوریاعظم کرمی 1 , ذبیح الله خاکسار 2
1 - گروه زیست‌شناسی، دانشگاه پیام نور، صندوق پستی 3697-19395، تهران، ایران
2 - گروه علوم تشریح، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران
کلید واژه: دیابت, مخ, استرپتوزوتوسین, مخچه, عصاره Panax ginseng,
چکیده مقاله :
دیابت بارداری عدم تحمل گلوکز با شدت متغیر است که اولین بار در طی بارداری شروع و یا تشخیص داده می‌شود و می‌تواند در بخش‌های مختلف سیستم عصبی مرکزی ایجاد اختلال کند. هدف از این پژوهش مطالعه اثر عصاره Panax ginseng بر تغییرات هیستومورفومتریک مخ و مخچه در نوزادان 14 روزه موش صحرایی مادران دیابتی بود. تعداد 16 موش صحرایی به چهار گروه مساوی شامل کنترل غیر دیابتی، غیر دیابتی دریافت‌کننده عصاره، کنترل دیابتی و دیابتی دریافت‌کننده عصاره تقسیم شد. دیابت در موش‌های گروه‌های دیابتی توسط داروی استرپتوزوتوسین القا گردید و هر چهار گروه با جفت‌گیری طبیعی باردار شدند. گروه‌های دریافت‌کننده عصاره در طول بارداری روزانه به‌میزان 400 میلی‌گرم بر کیلوگرم وزن بدن عصاره جینسنگ را به‌صورت خوراکی دریافت کردند. 14 روز پس از زایمان طبیعی، نوزادان بیهوش شدند. با ایجاد برش در جمجمه، مخ و مخچه خارج گردید. پس از بکارگیری روش‌های بافت‌شناسی، برخی فاکتورهای بافتی اندازه‌گیری گردید. در پایان اطلاعات به‌دست آمده با استفاده از نرم‌افزار SPSS و آزمون‌های آماری آنالـیز واریانس یک‌طرفه و تست دانکن مورد بررسی و تحلیل قرار گرفت (05/0p≤). ضخامت و تعداد سلول‌های ماده خاکستری مخ و تعداد سلول‌های ماده سفید مخچه در گروه کنترل دیابتی نسبت به دو گروه غیر دیابتی کاهش معنی‌دار داشت (05/0p≤). همچنین کاهش معنی‌داری در تعداد سلول‌های ماده سفید مخ در گروه کنترل دیابتی نسبت به سایر گروه‌ها وجود داشت (05/0p≤). عصاره Panax ginseng از طریق افزایش تحریک سلول‌های بتای پانکراس و تولید هورمون انسولین، قادر به کنترل هپیرگلیسمی در مادران دیابتی باردار و کاهش اختلالات حاصل از دیابت بر مخ و مخچه نوزادان آنها می‌شود.
Gestational diabetes is a variable amount of glucose intolerance initially diagnosed or diagnosed during pregnancy and can induce developmental disorders in different parts of CNS. We investigated effect of extract of Panax ginseng on histomorphometric changes oncerebrum and cerebellum in 14 d offspring'srat from diabetic mothers. Sixteen rats were divided into four groups: non-diabetic control, non-diabetic recipient of the extract, diabetic control and diabetic recipient of the extract. Diabetes was induced by streptozotocin in diabetic groups and all 4 groups became pregnant. During pregnancy, recipient of the extract groups received ginseng extract at the dose of 400 mg/kg of body weight every day. Overall, 14 d of normal delivery; offsprings were anesthetized. The cerebrum and cerebellum were removed by cutting the skull. After using the techniques of histology, some histological factors were measured. Data were analyzed using SPSS software and ANOVA and Duncan statistical tests (P≤0.05). A significant reduction was observed in gray matter thickness and cell count of cerebrum and white matter cell count of cerebellum in diabetic control group than non-diabetic groups (P≤0.05). Moreover, a significant reduction was observed in white matter cell count of cerebrum in diabetic control group than other groups (P≤0.01). Extract of P. ginseng could control hyperglycemia and manage diabetes on the cerebrum and cerebellum disorders in offsprings of mothers with gestational diabetes by increasing β-cells stimulation and increase insulin production.
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