اثر افزودن مکمل های پری‌بیوتیک، سین‌بیوتیک و فیتوبیوتیک در جیره بر عملکرد رشد، صفات لاشه، قابلیت هضم ظاهری مواد مغذی و برخی فراسنجه های خونی بره های پرواری نژاد زل
محورهای موضوعی : فصلنامه زیست شناسی جانوری
مهدی سراوانی
1
,
محسن حاجی پور
2
,
کاوه جعفری خورشیدی
3
,
سیدماکان موسوی کاشانی
4
,
پروین شورنگ
5
1 - گروه علوم دامی، واحد قائمشهر، دانشگاه آزاد اسلامی، قائمشهر، ایران
2 - گروه علوم دامی، واحد قائمشهر، دانشگاه آزاد اسلامی، قائمشهر، ایران
3 - گروه علوم دامی، واحد قائمشهر، دانشگاه آزاد اسلامی، قائمشهر، ایران
4 - گروه ژنتیک و اصلاح نژاد دام، سازمان جهاد کشاورزی استان تهران، تهران، ایران
5 - پژوهشکده کشاورزی هسته ای، پژوهشگاه علوم و فنون هسته ای سازمان انرژی اتمی ایران، تهران، ایرانایران
کلید واژه: عملکرد رشد, قابلیت هضم ظاهری, فراسنجههای خونی, مکمل پری‌بیوتیک, برههای پرواری,
چکیده مقاله :
در این تحقیق اثر افزودن مکمل‌های پری‌بیوتیک، سین‌بیوتیک و فیتوبیوتیک در جیره روی عملکرد رشد، صفات لاشه، قابلیت هضم‌ظاهری مواد مغذی و برخی فراسنجههای خونی برههای پرواری نژاد زل مورد بررسی قرار گرفت. بدین منظور از تعداد 24 رأس بره نر زل با میانگین سن حدود 5 ماه و با میانگین وزن 50/0±4/25 به مدت 90 روز استفاده شد. تیمارهای آزمایشی شامل 1-گروه شاهد (فاقد مکمل)، 2-تیمار حاوی 2 گرم مکمل پری‌بیوتیک ای-مکس، 3-تیمار حاوی 4 گرم مکمل سین‌بیوتیک بایومین ایمبو و 4-تیمار حاوی 6 گرم مکمل فیتوبیوتیک بیوهربال به ازای هر رأس بره در روز بود. نتایج عملکرد رشد نشان داد که در وزن پایان پروار، ماده خشک مصرفی، افزایش وزن روزانه و ضریب تبدیل خوراک تفاوت معنی‌داری بین تیمارهای آزمایشی وجود داشت (05/0 > p). بیشترین وزن پایان دوره پروار، ماده خشک مصرفی و افزایش وزن روزانه در تیمار 4 گرم مکمل سین‌بیوتیک مشاهده شد. تیمار 6 گرم مکمل فیتوبیوتیک دارای کمترین ضریب تبدیل خوراک بود. بالاترین قابلیت هضم ظاهری ماده خشک، الیاف نامحلول در شوینده خنثی و الیاف نامحلول در شوینده اسیدی در تیمار 4 گرم مکمل سین‌بیوتیک مشاهده شد (05/0 >p ). نتایج برخی فراسنجههای سرم خون شان داد که تفاوت معنی‌داری در غلظت گلوکز، کلسترول، تری‌گلیسرید و نیتروژن اوره‌ای خون بین تیمارهای آزمایشی وجود داشت (05/0> p). بالاترین غلظت گلوکز در تیمار 4 گرم مکمل سین‌بیوتیک، پایین‌ترین غلظت کلسترول در تیمار 6 گرم مکمل فیتوبیوتیک وجود داشت. نیتروژن اوره‌ای خون در تیمار 4 گرم مکمل سین‌بیوتیک دارای پایین‌ترین غلظت بود. تیمار 4 گرم مکمل سین‌بیوتیک دارای بالاترین وزن لاشه گرم، درصد لاشه گرم، وزن لاشه سرد، درصد لاشه سرد، درصد ران و درصد سردست بود (05/0> p). تحقیق حاضر نشان داد که افزودن سطح 4 گرم مکمل سین‌بیوتیک در جیره سبب بهبود عملکرد رشد، قطعات با ارزش لاشه و قابلیت هضم ظاهری مواد مغذی در برههای پرواری زل شد.
In this study, the effect of adding prebiotic, synbiotic and phytobiotic supplements in the diet on growth performance, carcass traits, apparent digestibility of nutrients and some blood parameters of fattening Zell lambs was investigated. For this purpose, 24 Zell male lambs with an avrage age of about 5 months and an avrage weight of 25.4±0.50 were used for 90 days. The experimental treatments included 1- control group (no supplement), 2- treatment containing 2 g of A-Max prebiotic supplement, 3- treatment containing 4 g of Biomin IMBO synbiotic supplement, and 4- treatment containing 6 g of Bioherbal phytobiotic supplement per head of lamb per day. The growth performance results showed that there was a significant difference between the experimental treatments in the fattening weight, dry matter intake, daily weight gain and feed conversion ratio (p < 0.05). The highest weight at the end of the fattening period, dry matter intake and daily weight gain were observed in the treatment of 4 g of synbiotic supplement. The treatment with 6 g of phytobiotic supplement had the lowest feed conversion ratio. The highest apparent digestibility of dry matter, NDF and ADF was observed in the treatment of 4 g of synbiotic supplement (p < 0.05). The results of some blood serum parameters showed that there was a significant difference in the concentration of glucose, cholesterol, triglyceride and blood urea nitrogen between the experimental treatments (p < 0.05). The highest concentration of glucose was in the treatment of 4 g of synbiotic supplement, the lowest concentration of cholesterol was in the treatment of 6 g of phytobiotic supplement. Blood urea nitrogen had the lowest concentration in the treatment of 4 g of synbiotic supplement. The treatment with 4 g of synbiotic supplement had the highest hot carcass weight, hot carcass percentage, cold carcass weight, cold carcass percentage, thigh percentage and shoulder percentage (p < 0.05). The present research showed that the addition of 4 g of synbiotic supplement in the diet improved the growth performance, valuable parts of the carcass and the apparent digestibility of nutrients in fattening lambs.
1. AOAC. 2003. Official methods of analysis of AOAC international. 17th edition.2nd revision. Association of Analytical Communities. Gaithersburg, MD, USA. Association of Analytical Communities.
2. Ayala-Monter M.A., Hernandez-Sanchez D., Pinto-Ruiz R., Torres-Salado N., Martinez-Aispuro J.A., Barcena-Gama, J.R., Caro-Hernandez J. M. 2019. Effect of inulin and Lactobacillus casei on productive performance, ruminal variables and blood metabolites in weaned lambs. Agrociencia, 53(3):303-317.
3. Ārne A., Ilgaža A. 2016. Different dose inulin feeding effect on calf digestion canal state and development. ReseaRch foR RuRal Development, 14:116-119.
4. Anderson W.G., McKinley R.S., and Colavecchia M. 1997. The use of clove oil as an anaesthetic for rainbow trout and its effects on swimming performance. North American Journal of Fisheries Management, 17:301-307.
5. Ai Q., Xu H., Mai K., Xu W., Wang J., Zhang W. 2011. Effects of dietary supplementation of Bacillus subtilis and fructooligosaccharide on growth performance, survival, non-specific immune response and disease resistance of juvenile large yellow croaker, Larimichthys crocea. Aquaculture, 317(4):155-161.
6. Bampidis V.A., Christodoulou V., Florou-Paneri P., Christaki E., Spais A.B., Chatzopoulou, P.S. 2005. Effect of dietary dried oregano leaves supplementation on performance and carcass characteristics of growing lambs. Animal Feed Science and Technology, 121(3):285-295.
7. Benchaar C., Calsamiglia S., Chaves A.V., Fraser G.R., Colombatto D., McAllister T.A., Beauchemin K.A. 2008. A review of plant-derived essential oils in ruminant nutrition and production. Animal Feed Science and Technology, 145(1):209-228.
8. Begley M., Hill C., Gahan C.G. 2006. Bile salt hydrolase activity in probiotics. Applied and Environmental Microbiology, 72:1729-1738.
9. Calsamiglia S., Busquet M., Cardozo P.W., Castillejos, L., Ferret A. 2007. Invited review: essential oils as modifiers of rumen microbial fermentation. Journal of Dairy Science, 90(6):2580-2595.
10. Chithra V., Leelamma S. 1997. Coriandrum sativum changes the levels of lipid peroxides and activity of antioxidant enzymes in experimental animals. Indian Journal of Biochemistry and Biophysiology, 36:59-61.
11. Chaves A.V., Sanford K., Gibson L.L., McAllister T.A., Benchaar C. 2008. Effect of carvacrol and cinnamaldehyde on intake, rumen fermentation, Growth performance and carcass characteristics of growing lambs. Journal of Animal Feed Science and Technology, 145:396-408.
12. Chashnidel Y., Bahari M., Yansari A.T., and Kazemifard M. 2020. The Effects of Dietary Supplementation of Prebiotic and Peptide on Growth Performance and Blood Parameters in Suckling Zell Lambs. Small Ruminant Research, 188:106121.
13. Daghash M.W., El-Ati M.A., Allam F.M., and Abbas S.F. 2014. Carcass characteristics of Saidi rams fed mannan oligosaccharide supplemented diet. Assiut Journal of Agricultural Sciences, 45:13-24.
14. Duncan D.B. 1955. Multiple range and multiple F tests. Biometrics, 1:1-42.
15. Elliethy M.A., Fattah A., Marwan A.A. 2022. Influence of prebiotic, probiotic and synbiotic supplementation on digestibility, haemobiochemical profile and productive performance in Barki lambs. Egyptian Journal of Nutrition and Feeds, 25(2):199-210.
16. El-Mehanna S.F., Abdelsalam M.M., Hashem N.M., El-Azrak K.E.M., Mansour M.M., Zeitoun M.M. 2017. Relevance of probiotic, prebiotic and synbiotic supplementations on hemato-biochemical parameters, metabolic hormones, biometric measurements and carcass characteristics of sub-tropical Noemi lambs. International Journal of Animal Research, 1:10-22.
17. El-Katcha M.I., Soltan M.A., and Essi M.S. 2016. Effect of Pediococcus spp. supplementation on growth performance, nutrient digestibility and some blood serum biochemical changes of fattening lambs. Alexandria Journal for Veterinary Sciences, 49(1):45-54.
18. Fraser T.J., Rowarth J.S. 1996. Legumes, herbs or grass for lamb performance? In Proceedings of the Journal of New Zealand Grassland Association, 58:49-52.
19. Ferket P.R. 2004. Alternatives to antibiotics in poultry production: responses, practical experience and recommendations. Nutritional Biotechnology in the Feed and Food Industries, 57-67.
20. Friedewald W.T., Levy R.I., Fredrickson, D.S. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry, 18(6):499-502.
21. Fayed A.M., El-Ashry M.A., Youssef K.M., Salem F.A. Aziz H.A. 2005. Effect of feeding falvomycin or yeast as feed supplement on ruminal fermentation and some blood constituents of sheep in Sinai. Egyptian Journal of Nutrition and Feeds. 8:619- 634.
22. Gardner H.G., Kaye P.L. 1991. Insulin increases cell numbers and morphological development in mouse pre-implantation embryos in vitro. Reproduction, Fertility and Development, 3:79-91.
23. Galvão K.N., Santos J.E., Coscioni A., Villaseñor M., Sischo W.M., Berge A.C.B. 2005. Effect of feeding live yeast products to calves with failure of passive transfer on performance and patterns of antibiotic resistance in fecal Escherichia coli. Reproduction Nutrition Development, 45(4):427-440.
24. Ghazanfar S., Anjum M.I., Azim A., Ahmed I. 2015. Effects of dietary supplementation of yeast (Saccharomyces cerevisiae) culture on growth performance, blood parameters, nutrient digestibility and fecal flora of dairy heifers. Journal of Animal and Plant Sciences, 25(25):53-9.
25. Gibson G.R., Fuller R. 2000. Aspects of in vitro and in vivo research approaches directed toward identifying probiotics and prebiotics for human use. Journal of Nutrition, 2:391-395
26. Ghosh S., Mehla R.K. 2012. Influence of dietary supplementation of prebiotics (mannanoligosaccharide) on the performance of crossbred calves. Tropical Animal Health and Production, 44(3):617-622.
27. Hajalizadeh Z., Dayani O., Khezri A., Tahmasbi R. 2020. Digestibility, ruminal characteristics, and meat quality of fattening lambs fed different levels of fennel (Foeniculum vulgare) seed powder. Journal of Livestock Science and Technologies, 8(1):37-46.
28. Hossain S.A., Parnerkar S., Haque N., Gupta R.S., Kumar D., Tyagi A. K. 2012. Influence of dietary supplementation of live yeast (Saccharomyces cerevisiae) on nutrient utilization ruminal and biochemical profiles of Kankrej calves. Journal of Applied Animal Research, 1:30-38.
29. Ilgaza A., Arne A. 2021. Comparative effect of different amount of inulin and symbiotic on growth performance and blood characteristics 12 weeks old calves. Agronomy Research, 19(4):1772–1780.
30. Jang I.S., Ko Y.H., Yang H.Y., Ha J.S., Kim J.Y., Kim J.Y., Lee C.Y. 2004. Influence of essential oil components on growth performance and the functional activity of the pancreas and small intestine in broiler chickens. Asian-Australasian Journal of Animal Sciences, 17(3):394-400.
31. Jin L.Z., Ho Y.W., Abdullah N., Jalaudin S. 1996. Influence of dried Bacillus substillis and Lactobacilli cultures on intestinal microflora and performance in broilers. Asian-Australasian Journal of Animal Sciences, 9(4):397-404.
32. Kazemi-Bonchenari M., Ghasemi H.A., Khodaei-Motlagh M., Khaltabadi-Farahani A.H., Ilani M. 2013. Influence of feeding synbiotic containing Enterococcus faecium and inulin on blood metabolites, nutrient digestibility and growth performance in sheep fed alfalfa-based diet. Scientific Research and Essays, 8:853-857
33. Kim M.H., Seo J.K., Yun C.H., Kang S.J., Ko J.Y., and Ha J.K. 2011. Effects of hydrolyzed yeast supplementation in calf starter on immune responses to vaccine challenge in neonatal calves. Animal, 5(6):953-960.
34. Khalid M.F., Shahzad M.A., Sarwar M., Rehman A.U., Sharif M. Mukhtar, N. 2011. Probiotics and lamb performance: A review. African Journal of. Agricultural Research, 6:5198- 5203.
35. Kianbakht S., Jahaniani F. 2003. Evaluation of antibacterial activity of Tribulus terrestris L. growing in Iran. Iranian Journal of Pharmacology and Therapeutics, 2:22-24.
36. Kholif S.M., Morsy T.A., Abdo M.M., Mattlloup O.H., Abu El-Ela A.A. 2012. Effect of supplementing lactating goat’s rations with garlic, cinnamon or ginger oils on milk yield, milk composition and milk fatty ccids profile. Journal of Life Sciences, 4:27-34.
37. Ling H., Xiao H., Zhang Z., He Y., and Zhang P. 2023. Effects of macleaya cordata extract on performance, nutrient apparent digestibilities, milk composition, and plasma metabolites of dairy goats. Animals, 13(4):566 -580.
38. McDonald P., Edwards R.A., Greenhalgh J.F.D., Morgan C.A. 2002. Animal nutrition. (6th ed.), Prentice Hall, Publishers Ltd., UK.
39. Mohammed N., Ajisaka N., Lila Z.A., Hara K., Mikuni K., Hara K., Itabashi H. 2004. Effect of Japanese horseradish oil on methane production and ruminal fermentation in vitro and in steers. Journal of Animal Science, 82(6):1839-1846.
40. Moarrab A., Ghoorchi T., Ramezanpour S., Ganji F., Koochakzadeh A.R. 2016. Effect of synbiotic on performance, intestinal morphology, fecal microbial population and blood metabolites of suckling lambs. Iranian Journal of Applied Animal Science, 6(3):621-628
41. Nikbakht S.A., Mohammadabadi T., Mirzadeh K. 2021. The effect of feeding Tribulus terrestris plant powder on growth performance, digestibility, rumen and blood parameters of Iranian Arabic lambs. Iranian Journal of Applied Animal Science, 11(4):781-788.
42. Özdoğan M., Önenç S.S., Önenç A. 2011. Fattening performance, blood parameters and slaughter traits of Karya lambs consuming blend of essential oil compounds. African Journal of Biotechnology, 10(34):6663-6669.
43. Quigley J.D., Kost C.J., and Wolfe T.A. 2002. Effects of spray-dried animal plasma in milk replacers or additives containing serum and oligosaccharides on growth and health of calves1. Journal of Dairy Science, 85(2):413-421.
44. Rahchamani R., Ghanbari F., Mostafalo Y., and Ghasemifard M. 2017. Effects of Matricaria chamomille and Cichorium intybus powder on performance, rumen microbial population and some blood parameters of Dallagh sheep. Iranian Journal of Veterinary Medicine, 11(3):267-277.
45. Roberfroid M. 2002. Functional food concept and its application to prebiotics. Digestive and Liver Disease, 34:105-110.
46. Roodposhti P.M., Dabiri N. 2012. Effects of probiotic and prebiotic on average daily gain, fecal shedding of Escherichia coli, and immune system status in newborn female calves. Asian-Australasian Hournal of Animal Sciences, 25(9):1255.
47. SAS. 2001. Statistical Analysis System User's Guide: Statistics. SAS Institute, Cary, NC.
48. Somasiri S.C. 2014. Effect of herb-clover mixes on weaned lamb growth: a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Palerston North, New Zealand (Doctoral dissertation, Massey University).
49. Soliman S.M., El-Shinnawy A.M., El-Morsy A.M. 2016. Effect of probiotic or prebiotic supplementation on the productive performance of Barki lambs. Mansoura Journal of Animal and Poultry Production, 7(10):369-376.
50. Tedeschi L.O., Cannas A., Fox D.G. 2010. A nutrition mathematical model to account for dietary supply and requirements of energy and other nutrients for domesticated small ruminants: The development and evaluation of the Small Ruminant Nutrition System. Small Ruminant Research, 89(2-3):174-184.
51. Thomas L. 1998. Clinical laboratory diagnostics. 1 st English ed. Frankfurt/Main; TH-Books-Verl-Ges, 548-640.
52. Terre M., Pedrals E., Dalmau A., Bach, A. 2013. What do preweaned and weaned calves need in the diet: high fiber content or a forage source. Journal Dairy Science, 96:5217-5225.
53. Van Keulen J., Young B.A. 1977. Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. Journal of Animal Science, 44:282-287.
54. Vahabzadeh M., Chamani M., Dayani O., Sadeghi A.A., Mohammadabadi M.R. 2021. Effects of sweet marjoram (Origanum majorana) powder on growth performance, nutrient digestibility, rumen fermentation, meat quality and humoral immune response in fattening Lambs. Iranian Journal of Applied Animal Science, 11(3):567-576.
55. Vanhatalo A., Varvikko T., Huhtanen P. 2003. Effects of casein and glucose on responses of cows fed diets based on restrictively fermented grass silage. Journal of Dairy Science, 10:3260-3270.
56. Van Soest P.V., Robertson J. B., Lewis B.A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10):3583-3597.
57. Windisch W., Schedle K., Plitzner C., Kroismayr A. 2008. Use of phytogenic products as feed additives for swine and poultry. Journal of Animal Science, 86:140-148.
58. Yan W., Ohtani K., Kasai R., Yamasaki K. 1996. Steroidal saponins from fruits of Tribulus terrestris. Phytochemistry. 42:1417-1422.
59. Yang W.Z., Benchaar C., Ametaj B.N., Chaves A.V., He M.L., McAllister, T.A. 2007. Effects of garlic and juniper berry essential oils on ruminal fermentation and on the site and extent of digestion in lactating cows. Journal of Dairy Science, 90(12):5671-5681.
60. Yang W.Z., Ametaj B.N., Benchaar C., He M.L., Beauchemin K.A. 2010. Cinnamaldehyde in feedlot cattle diets: intake, growth performance, carcass characteristics, and blood metabolites. Journal of Animal Science, 88(3):1082-1092
61. Zheng C., Li F., Hao Z., Liu T. 2018. Effects of adding mannan oligosaccharides on digestibility and metabolism of nutrients, ruminal fermentation parameters, immunity, and antioxidant capacity of sheep. Journal of Animal Science, 96(1):284-292.