اثر تمرین استقامتی بعد از القای آلزایمر بر برخی عوامل مرتبط با نوروپلاستیسیته در هیپوکمپ موش های نر نژاد ویستار

محورهای موضوعی : فصلنامه زیست شناسی جانوری

سجاد رجبی امیری ¹ , علیرضا براری ² , احمد عبدی ³

1 - گروه فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
2 - گروه فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
3 - گروه فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

تاریخ دریافت : 1400/03/25 تاریخ پذیرش : 1400/06/21 تاریخ انتشار : 1400/12/01

کلید واژه: آلزایمر, BDNF, هیپوکمپ, PKG, cGMP,

چکیده مقاله :

میلیون ها نفر در سراسر جهان به بیماری آلزایمر مبتلا هستند و با رشد نامتناسب جمعیت مسن، این بیماری که رایج ترین شکل زوال عقل در میان افراد مسن است، در حال تبدیل شدن به یک بحران سلامتی عمومی است. هدف از پژوهش حاضر بررسی اثر تمرین استقامتی قبل و بعد از القای آلزایمر بر برخی عوامل مرتبط با نوروپلاستیسیته در هیپوکمپ موش های نر نژاد ویستار بود. 32 سر رت نر بالغ 8 هفته ای با میانگین وزنی 17 ± 250 گرم، قبل از القای آلزایمر به صورت تصادفی به 2 گروه استراحت (16 سر) و تمرین (16 سر) تقسیم شدند. پس از 4 هفته (هفته اول و دوم با سرعت 10 متر بر دقیقه در دو نوبت 15 دقیقه‌ای، هفته سوم با سرعت 15 متر بر دقیقه در سه نوبت 15 دقیقه‌ای و در هفته چهارم با سرعت 15 متر بر دقیقه در چهار نوبت 15 دقیقه‌ای با وقفه 5 دقیقه‌ای)، هر گروه به دو زیرگروه: 1. تزریق آمیلوئیدبتا و 2. بدون تزریق تقسیم شدند. پس از 72 ساعت حیوانات کشته و هیپوکمپ آن ها جهت بررسی برداشته شد. بیان ژن BDNF، PKG و cGMP با روش Real Time PCR اندازه گیری شد. داده ها به روش تحلیل واریانس یک طرفه و آزمون تعقیبی توکی در سطح معنی داری 05/0 p < تجزیه و تحلیل شدند. نتایج نشان داد که سطح BDNF، PKG و cGMP در گروه تمرین نسبت به گروه استراحت در مرحله بعد از القای آلزایمر به طوری معنی داری بیشتر بود (001/0= p). با توجه به نتایج، به نظر می رسد تمرین هوازی می تواند به بهبود نوروپلاستیسیته در هیپوکمپ موش های آلزایمری کمک کند.

چکیده انگلیسی:

Millions of people worldwide have Alzheimer's disease, and with the disproportionate growth of the elderly population, the disease, which is the most common form of dementia among the elderly, is becoming a public health crisis. This study aimed to investigate the effect of endurance training before and after Alzheimer's induction on some neuroplasticity-related factors in the hippocampus of male Wistar rats. Before inducing Alzheimer's disease, 32 8-week-old male rats weighing an average of 250 g were randomly allocated into two groups: rest (16 heads) and exercise (16 heads). Each group was divided into two subgroups after four weeks (the first and second weeks at a speed of 10 meters per minute in two 15-minute shifts, the third week at a speed of 15 meters per minute in three 15-minute shifts, and the fourth week at a speed of 15 meters per minute in four 15-minute shifts with 5-minute intervals).1. amyloid beta injection and 2. without injection. After 72 hours, the animals were killed and their hippocampus was removed for examination. Real Time PCR measured BDNF, PKG, and cGMP gene expression.Data were analyzed by one-way analysis of variance and Tukey post hoc test at the significant level of p <0.05. The results showed that the levels of BDNF, PKG and cGMP in the exercise group were significantly higher than the rest group in the stage after Alzheimer's induction (p = 0.001). According to the results, it seems that aerobic exercise can help improve neuroplasticity in the hippocampus of Alzheimer's mice.

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