بررسی اثرات عصاره آبی گیاه شاهی Lepidium sativum بر روی رفتارهای شبه اضطرابی و شبه افسردگی در موش های سوری نر بالغ

محورهای موضوعی : فصلنامه زیست شناسی جانوری

فاطمه رحیمی اصل ¹ , مریم خسروی ² , رامین حاجی خانی ³ , جلال صولتی ⁴ , حسین فهیمی ⁵

1 - گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
3 - گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
4 - گروه زیست شناسی، واحد گوهر دشت کرج، دانشگاه آزاد اسلامی، کرج، ایران
5 - گروه ژنتیک، واحد علوم پزشکی، دانشگاه آزاد اسلامی، تهران، ایران

تاریخ دریافت : 1399/06/04 تاریخ پذیرش : 1399/09/17 تاریخ انتشار : 1400/06/01

کلید واژه: اضطراب, افسردگی, موش سوری, شاهی,

چکیده مقاله :

اضطراب و افسردگی از شایعترین بیماری های روانی هستند. استرس اکسیداتیو از جمله عوامل ایجاد اضطراب و افسردگی است. دانه شاهی دارای خواص ضد التهابی، آنتی اکسیدانی می باشد. هدف از این تحقیق بررسی اثر عصاره آبی گیاه شاهی بر رفتارهای شبه اضطرابی و شبه افسردگی در موش های سوری نر بالغ است. در این تحقیق از موش های کوچک نر با محدوده وزنی 35-30 گرم استفاده شد. ابتدا حیوانات مورد آزمایش به مدت 2 هفته تحت تیمار با عصاره آبی دانه شاهی با دوز های 200 ،400 و 600 میلی گرم بر کیلوگرم قرار گرفتند. پس از پایان دوره تیمار، سطوح اضطراب و افسردگی به ترتیب به وسیله دستگاه ماز بعلاوه مرتفع (EPM) و تست شنای اجباری ارزیابی شد. در هیچ یک از گروه های دریافت کننده عصاره شاهی تغییرات معنی داری در زمان سپری شده در بازوی باز ، تعداد ورود به بازوی باز و فعالیت حرکتی مشاهده نشد. نتایج حاصل از تست شنای اجباری نشان داد که در حیوانات دریافت کننده عصاره شاهی با دوزهای 400 و 600 میلی گرم بر کیلوگرم زمان شنا و زمان بی حرکتی نسبت به گروه کنترل به ترتیب افزایش و کاهش معنی داری داشته است (01/0 p <). یافته های حاصل از این مطالعه اثرات تعدیل کنندگی عصاره دانه شاهی بر رفتارهای شبه افسردگی را نشان داد، اما این عصاره بر رفتارهای شبه اضطرابی اثرات معنی داری نداشت.

چکیده انگلیسی:

Anxiety and depression are the most common mental illnesses. Oxidative stress is one of the causes of anxiety and depression. Lepidium sativum has anti-inflammatory and antioxidant properties. The aim of this study was to investigate the effect of aqueous extract of Lepidium sativum on anxiety-like and depression-like behaviors in adult male mice. In this study, male mice weighing 30-35 g were used. First, the tested animals were treated with aqueous extract of Lepidium sativum at doses of 200, 400 and 600 mg/kg for 2 weeks. At the end of the treatment period, the levels of anxiety and depression were assessed by an elevated plus maze (EPM) and forced swimming test, respectively. No significant changes were observed in any of the groups receiving Lepidium sativum extract in the time spent in the open arm, the number of entrances to the open arm and motor activity. The results of forced swimming test showed that in animals receiving Lepidium sativum extract at doses of 400 and 600 mg/kg, swimming time and immobility time were significantly increased and decreased compared to the control group, respectively (p < 0.01). The results of this study showed the modulatory effects of Lepidium sativum extract on depressive-like behaviors, but this extract had no significant effects on anxiety-like behaviors.

منابع و مأخذ:

1. Atarodi A., Lotfi G.R., Mottaghi M., Daemi A., Rohani Z. 2011. The Relationship Between Coaching Anxiety with Footsal Players’ Anxiety and Performance in Gonabad City in 2010. The Horizon of Medical Sciences, 17(3): 58-66. [in Persian]


2. Babri S., Doosti M., Fatehi L., Salari A. 2012. The effects of Scrophularia striata extract on anxiety and depression behaviors in adult male mice. Tabriz Univ Med Sci,18(2):133-40. [in Persian]


3. Bahrami S., Razi Jalali M., Ramezani Z., Pourmehdi Boroujeni M., Toeimepour F. 2016. In vitro scolicidal effect of Lepidium sativum essential oil. Journal of Ardabil University of Medical Sciences, 15(4): 395-403. [in Persian]


4. Bajpai A., Verma A.K., Srivastava M., Srivastava R. 2014. Oxidative stress and major depression. Journal of clinical and diagnostic research: JCDR, 8(12): CC04.


5. Beesdo K., Knappe S ., Pine DS. 2009. Anxiety and anxiety disorders in children and adolescents: developmental issues and implications for DSM-V. Psychiatric Clinics, 32(3): 483-524.


6. Black C.N., Bot M., Scheffer P.G., Cuijpers P., Penninx B.W. 2015. Is depression associated with increased oxidative stress? A systematic review and meta-analysis. Psychoneuroendocrinology, 51: 164-175.


7. Blondeau N., Nguemeni C., Debruyne DN., Piens M., Wu X., Pan H., Hu X., Gandin C., Lipsky RH., Plumier J-C. 2009. Subchronic alpha-linolenic acid treatment enhances brain plasticity and exerts an antidepressant effect: a versatile potential therapy for stroke. Neuropsychopharmacology, 34(12): 2548-2559.


8. Bouayed J., Rammal H., Soulimani R. 2009. Oxidative stress and anxiety: relationship and cellular pathways. Oxidative medicine and cellular longevity, 2.


9. Butterweck V, Jurgenliemk G, Nahrstedt A, Winterhoff H. Flavonoids from Hypericum perforatum show antidepressant activity in the forced swimming test. Planta medica. 000;66(1):3-6.


10. Dannehl D., Huyskens-Keil S., Wendorf D., Ulrichs C., Schmidt U. 2012. Influence of intermittent-direct-electric-current (IDC) on phytochemical compounds in garden cress during growth. Food chemistry, 131(1): 239-246.


11. Diwakar B.T., Dutta P.K., Lokesh B.R., Naidu KA. 2010. Physicochemical properties of garden cress (Lepidium sativum L.) seed oil. Journal of the American Oil Chemists' Society, 87(5): 539-548.


12.  Eddouks M., Maghrani M., Zeggwagh N-A., Michel J. 2005. Study of the hypoglycaemic activity of Lepidium sativum L. aqueous extract in normal and diabetic rats. Journal of Ethnopharmacology, 97(2): 391-395.


13. Fißler M., Quante A. 2014. A case series on the use of lavendula oil capsules in patients suffering from major depressive disorder and symptoms of psychomotor agitation, insomnia and anxiety. Complementary Therapies in Medicine, 22(1): 63-69.


14. Gay B.M., Prigol M., Stein AL., Nogueira C.W. 2010. Antidepressant-like pharmacological profile of 3-(4-fluorophenylselenyl)-2, 5-diphenylselenophene: Involvement of serotonergic system. Neuropharmacology, 59(3): 172-179.


15. Hamidpour M., Hamidpour R.,Hamidpour S., Shahlari M. 2014. Chemistry,pharmacology, and medicinal property ofsage (Salvia) to prevent and cure illnesses  as obesity, diabetes, depression, dementia, lupus, autism, heart disease, and cancer. Journal of Traditional and Complementary Medicine, 4(2): 82-8.


16. Harati E., Sadeghipour Roodsari HR., Seifi B., Kamalinejad M., Nikseresht S. 2014. The effect of oral Matricaria Chamomilla extract and selenium on postpartum depression and plasma oxidant-antioxidant system in mice. Tehran University Medical Journal TUMS Publications, 71(10): 625-634. [in Persian]


17.  Hashemi S.F., Namjou A., Ghasemi P.A., Lorigooini Z., Rafafieian  K.M., Gholami A.M. 2017. Antidepressant-like effect of  Lavandula angustifolia Mill and Citrus aurantium Duh essential oils with forced swimming test in reserpinized mice balb/c. [in Persian]


18. Heidarian E., Movahed-Mohammadi G., Saffari J., Ghatreh-Samani K. 2013. Protective effect of hydroethanolic extract of cress against hepatotoxicity due to acetaminophen in rats. Journal of Mazandaran University of Medical Sciences, 23(102): 78-90. [in Persian]


19. Kamani M., Hosseini E.S., Kashani H.H., Atlasi M.A., Nikzad H. 2017. Protective Effect of Lepidium sativum Seed Extract on Histopathology and Morphology of Epididymis in Diabetic Rat Model. International Journal of Morphology.35(2): :603-610. [in Persian]


20. Kasabe P.J., Patil P.N., Kamble D., Dandge P.B. 2012. Nutritional, elemental analysis and antioxidant activity of garden cress (Lepidium sativum L.) seeds. Int J Pharm Pharm Sci, 4(3): 392-395.


21. Khakpour S., Khosravi M., Masoompoour A.S. 2009. Antidepressant effect of red ginseng  hydro-alcoholic extract by forced swimming and tail suspension tests in female mice. Biological sciences, 4(1):15-20. [in Persian]  


22. Miladi Gorgi H., Safakhah HA., Haghighi S. 2010. Anxiolytic effects of the aqueous extracts of spinach leaves in mice. Scientific Journal of Kurdistan University of Medical Sciences, 43-50.


23. Milajerdi A., Akhonzadeh S., Jazayeri S., Shirzadi E., Hashemzadeh N., Derakhshan Z., Djazayeri A., Mahmoodi M. 2016. The effect of saffron (Crocus sativus L.) hydro-alcoholic extract on mild to moderate mixed depression-anxiety treatment in type 2 diabetes: a triple-blind randomized placebo-controlled clinical trial. Iranian Journal of Nutrition Sciences & Food Technology, 11(3): 11-20. [in Persian]


24. Monahan F., Gray J., Asghar A., Haug A., Shi B., Buckley D., Morrissey P. 1993. Effect of dietary lipid and vitamin E supplementation on free radical production and lipid oxidation in porcine muscle microsomal fractions. Food Chemistry, 46(1): 1-6.


25. Mostafapour P., Asle-Rousta M., Rahnema M. 2018. Anxiolytic and antidepressant effects of Basil (Ocimum basilicum L.) hydro-alcoholic extract in male rats exposed to chronic restraint stress. SSU_Journals, 25(11): 887-896. [in Persian]


26. Najafi Doulatabad S., Hashemi N., Mohebi Nobandegani Z., Sadeghi H., Jafari A., Najafi Doulatabad A., Zoladle M. 2009. The effect of hydroalcoholic extract of mentha piperita (complement treatment) on severity of depressed patients. Armaghane danesh, 14(3): 83-90.


27. Ng H.P., Wang Y.F., Lee C.Y., Hu M.L. 1999. Toxicological and antioxidant effects of short-term dehydroepiandrosterone injection in young rats fed diets deficient or adequate in vitamin E. Food and chemical toxicology, 37(5):503-8


28. Omidvari S., Azin A., Montazeri A., Sadighi J., Jahangiri K., Aeenparast A., Farzadi F., Ebadi M., Maftoon F., Vahdaninia M. 2011. Trait anxiety among Iranians: a nationwide study. Payesh (Health Monitor), 10(3): 373-380.


29. Patro G., Bhattamisra S.K., Mohanty BK. 2016.  Effects of Mimosa pudica L. leaves extract on anxiety, depression and memory. Avicenna Journal of Phytomedicine, 6(6): 696.


30. Rabiee Z., Davoodizadeh Z., Bijad E., Rafieian-kopaei M. 2016. Evaluation of the effects of grape seed oil on the anxiety level and motor coordination of male wistar rats. Journal of Babol University of Medical Sciences, 18(6): 52-58.


31. Rai D., Bhatia G., Palit G., Pal R., Singh S., Singh H.K. 2003. Adaptogenic effect of Bacopa monniera (Brahmi). Pharmacology Biochemistry and Behavior, 75(4): 823-30


32. Ranjbaran M., Roudsari H.R.S., Nikseresht S., Etebary S. 2015. Antioxidant status and endocannabinoid concentration in postpartum depressive women. Tehran University Medical Journal, 72(11): [in Persian]


33. Rehman N.U., Khan A.U., Alkharfy K.M., Gilani A.H. 2012. Pharmacological basis for the medicinal use of Lepidium sativum in airways disorders. Evidence-Based Complementary and Alternative Medicine, 2012.


34. Saki K., Bahmani M., Rafieian-Kopaei M., 2014. The effect of most important medicinal plants on two importnt psychiatric disorders (anxiety and depression)-a review. Asian Pacific journal of tropical medicine 7: S34-S42.[in Persian]


35. Saki M., Jariani M., Saki K., Delfan B., Tarahi M., Gholami M. 2009. Effects of evening primrose oil on depression disorders on patients at the psycho-neurological clinic of Khoramabad.  Journal of Ilam University of Medical Sciences, 16 (4): 46-54. [in Persian]


36. Sayyah M., Sayyah M., Kamalinejad M. 2006. A preliminary randomized double blind clinical trial on the efficacy of aqueous extract of Echium amoenum in the treatment of mild to moderate major depression. Progress in Neuro-Psychopharmacology and Biological Psychiatry,30(1):166-9.


37. Salehi L., Farrokhi F.,  Hosseini H. 2014. Effect of Citalopram and Vitamin E on Pyramidal Neurons of the Hippocampus. Qom University of Medical Sciences Journal, 7(6): 14-20.


38. Scapagnini G., Davinelli S., Drago F., De Lorenzo A., Oriani G. 2012. Antioxidants as antidepressants. CNS drugs, 26(6): 477-490.


39. Selek S., Koyuncu I., Caglar HG., Bektas I., Yilmaz MA., Gonel A., Akyuz E. 2018. The evaluation of antioxidant and anticancer effects of Lepidium Sativum Subsp Spinescens L. methanol extract on cancer cells. Cellular and Molecular Biology, 64(3): 72-80


40. Shafei Z., Abbasi Maleki S., Ghaderi-Pakdel F. 2018. Evaluation of the antidepressant-like effect of Viola odorata hydroalcoholic extract in male mice. Journal of Birjand University of Medical Sciences, 25(4): 286-296. [in Persian]


41. Shahinfar J., Zeraati H., Nasimi F., Shojaei S. 2017. Effect of Medicinal Plants on anxiety, A review article. Journal of Islamic and Iranian Traditional Medicine, 8(2):209-22. [in Persian]


42. Singh M., Sandhir R., Kiran R.2011. Effects on antioxidant status of liver following atrazine exposure and its attenuation by vitamin E. Experimental and Toxicologic Pathology, 63(3): 269-276.


43. Solati J., Oryan S., Parivar K., Zarindast M.R. 2009. Effects of amygdala cholinergic system on modulation of anxiety behaviors of rats by use of Elevated Plus Maze test. Scientific Journal of Kurdistan University of Medical Sciences, 14(1). [in Persian]


44. Su K.P. 2009. Biological Mechanism of Antidepressant Effect of Omega–3 Fatty Acids: How Does Fish Oil Act as a ‘Mind-Body Interface’? Neurosignals, 17(2):144-52.


45. Su K.P., Matsuoka Y., Pae C.U. 2015. Omega-3 polyunsaturated fatty acids in prevention of mood and anxiety disorders. Clinical Psychopharmacology and Neuroscience,13(2):129.


46. Zarei M., Mohammadi S., Jabbari S., Shahidi S. 2019. Intracerebroventricular microinjection of  kaempferol on memory retention of passive  avoidance learning in rats: involvement of  cholinergic mechanism(s).  Int J Neurosci, 129: 11-23.