اثر هشت هفته تمرین هوازی و مصرف مکمل رزوراترول بر نشانگر اکسایشی (MDA) و آنتی اکسایشی(SOD) و (GPX) بافت کاردیومیوسیتی رت های نر دیابتی شده با استرپتوزوتوسین
محورهای موضوعی : مجله پلاسما و نشانگرهای زیستی
1 - سوپروایزر بالینی مجتمع اموزش درمانی بوعلی .
دانشگاه ازاد علوم پزشکی تهران
کلید واژه: دیابت, MDA, تمرین هوازی, SOD, رزوراترولGPX,
چکیده مقاله :
چکیده زمینه و هدف: مطالعه حاضر اثر تمرین هوازی همراه مصرف مکمل رزوراترول بر فاکتورهای تنظیمی و اجرایی آپوپتوز کاردیومیوسیتی در رت های نر دیابتی شده با استرپتوزوسین است. روش کار: دریک کارآزمایی تجربی 42 موشصحرایی نر، تصادفی به 6 گروه7 تایی کنترل سالم، دیابتی ، دیابتی سالین ، مکمل رزوراترول ، تمرین هوازی و مکمل رزوراترول+تمرین هوازی تقسیم شدند. دیابت با تزریق تک دوزml/kg50 استرپتوزوتوسین القا شد. برنامه گروه های تمرین، تمرین و مکمل بر روی تریدمیل 5 روز در هفته که به مدت 8 هفته انجام شد پس از پایان دوره تمام حیوانات با شرایط کاملاً مشابه و به دنبال12-14 ساعت ناشتایی و 48 ساعت پس از آخرین جلسه تمرینی بیهوش و قربانی شدند. پس از خروج قلب و هموژنایز کردن آن غلظت (GPX،SOD،MDA) بصورت الایزا سنجش شد. یافته ها: پس از القای دیابت غلضت GPX و SODقلبی نسبت به کنترل سالم کاهش معنی دار یافت در حالیکه غلظت MDAدر این شرایط افزایش معنی دار داشت .اما تعامل تمرین و رزوراترول توانست اثر معنی داری بر غلظت SOD قلبی داشته باشد در حالی که تعامل این دو اثر معنی دار بر غلظت MDAوGPX قلبی نداشت . نتیجه گیری: تمرین هوازی و رزوراترول اثر سودمندی بر آپوپتوز قلبی در القای دیابت دارند اما تعامل این دو بر روند آپوپتوز قلبی معنادار نیست. البته از تمرین هوازی و رزوراترول میتوان بعنوان طب مکمل برای حفاظت میوکارد در برابر آپوپتوز نابجای ناشی از دیابت القا شده استفاده نمود.
Inroduction & Objective:The present study was conducted to investigate the effect of aerobic training with resveratrol supplementation on regulatory and executive factors of cardiomyocytes apoptosis in streptococci diabetic male rats Material and Method:In an experimental study, 50 male rats were randomly divided into 5 groups of 10 healthy controls, diabetics, resveratrol supplements, aerobic training and complementary resveratrol + aerobic training. Diabetes was induced by single injection of streptozotocin, ml / kg. The program of exercise, exercise and supplementation groups began for 8 weeks and 5 days a week on the training of Treadmill. All animals were completely and similarly treated, followed by 14-12hours of fasting and 48 hours after the last training session, anesthetized and sacrificed. After cardiac output and homogenization, the concentration (GPX, SOD, and MDA) was measured as ELISA. Results: After induction of diabetes, the concentration of GPX and SOD in the cardiac system was significantly lower than healthy controls, while MDA concentration increased significantly in these conditions. However, exercise interaction and resveratrol had a significant effect on the concentration of cardiac SOD, while the interaction of these two effects there was no concentration of MDA and GPX in the heart. Conclusion: Aerobic exercise and resveratrol have a beneficial effect on cardiac apoptosis in inducing diabetes, but their interaction is not significant in the process of cardiac apoptosis. Of course aerobic exercise and resveratrol can be used as complementary medicine for protecting myocardial cells against the invasive induced apoptosis induced by diabetes.
1.American Diabetes Association, Diagnosis and classification of diabetes mellitus, Diabetes Care 33 (2010) 62e69.
2.Ascensao, A., Ferreira, R., Magalhaes, J. (2007). Exercise-induced cardioprotection biochemical morphological and functional evidence in whole tissue and isolated mitochondria. J physiol., 117(1); 16-30.
3.Bavaresco, L., Petegolli, D., Cantu, E., Fregoni, M., Chiusa, G., Trevisan, M. (1997)Elcitation and accumulation of stilbene phytoalexins in grapevine berries infected by Botrytis cinerea Vitis. Italian J. Food Sci., 36 (2); 77-83.
4.Baynes, JW. (1991). Role of oxidative stress in development of complication in diabetes. Diabetes, 40; 405-12.
5.Bo, S., Ciccone, G., Castiglione, A., Gambino, R., De Michieli, F., Villois, P, Durazzo M, Cavallo-Perin P, .(2013). Anti-inflammatory and antioxidant effects of resveratrol in healthy smokers a randomized, double-blind, placebo-controlled, cross-over trial. Current medicinal Chemistry, 20(10); 1323-31.
6.Chao, M., Zou, D., Zhang, Y., Chen, Y., Wang, M., Wu H. (2009). Improving insulin resistance with traditional Chinese medicine in type 2 diabetic patients. Endocrine, 36(2); 268-74.
7.Das, S., Alagappan, VK., Bagchi, D., Sharma, HS., Maulik, N., Das, DK. (2005). Coordinated induction of iNOS–VEGF–KDR–eNOS after resveratrol consumption: A potential mechanism for resveratrol preconditioning of the heart. Vascular Pharmacology, 42(5-6); 281-9.
8. De Michieli. (2002). Diabetes prevention program research group. reduction in the incidence of type 2 diabetes with lifestyle intervention or metformin. N Engl J Med, 346; 393-403.
9.Dolinsky, VW., Rogan, KJ., Sung, MM., Zordoky, BN., Haykowsky, MJ., Young, ME. (2013). Both aerobic exercise and resveratrol supplementation attenuate doxorubicin-induced cardiac injury in mice. American Journal of Physiology-Endocrinology and Metabolism, 305(2); E243-53.
10.Dehghan, F., Soori, R., Gholami, K., Abolmaesoomi, M., Yusof, A., Muniandy, S. (2016). Purslane(Portulacao leracea) seed consumption and aerobic training improves biomarkers associated with atherosclerosis in women with type 2 diabetes (T2D). Scientific Reports, 6; 37819.
11.Demirel-Yilmaz, E., Buluc, M., Ayaz, M., Turan, B. (2007). Resveratrol-induced depression of the mechanical and electrical activities of the rat heart is reversed by glyburide: evidence for possible K ATP channels activation. Archives of Pharmacal Research, 30(5); 603-7.
12.Ememe, MU., Mshelia, WP., Ayo, JO. (2015). Ameliorative effects of resveratrol on oxidative stress biomarkers in horses. Journal of Equine Veterinary Science, 35(6); 518-523.
13.Fregoni, M., Rajaei, F., Honardoost, M., Soleimanjahi, H. (2013). Apoptosis: programmed cell death. Review Article, JQUMS, 3; 48-57.
14.Fadini, GP., Iori, E., Marescotti, MC., de Kreutzenberg, SV., Avogaro, A. (2014). Insulin-induced glucose control improves HDL cholesterol levels but not reverse cholesterol transport in type 2 diabetic patients. Atherosclerosis, 235(2); 415-7.
15.Jackson, MJ. (1999). Free radicals in skin and muscle: damaging agents or signals for adaptation? Proc Nutr Soc., 58(3); 673-6
16.Korb, A., Cechinel, LR., Bertoldi, K., Delevatti, RS., Meireles, LCFd., Moysés, FdS. (2015). Effects of acute exercise and periodised training performed in different environments on oxidative status parameters in patients with type 2 diabetes. Anais., 1(12); 1-10.
17.Linden, MA., Fletcher, JA., Morris, EM., Meers, GM., Laughlin, MH., Booth, FW. (2015). Treating NAFLD in OLETF rats with vigorous-intensity interval exercise training. Medicine and Science in Sports and Exercise., 47(3); 556.
18.Maybaum, S., Ilan, M., Mogilevsky, J., Tzivoni, D. (1996). Improvement in ischemic parameters during repeated exercise testing: a possible model for myocardial preconditioning. The American Journal of Cardiology. 78(10); 1087-91.
19.Mirfeizi, M., Mehdizadeh, Tz., Rezvani, H., Asghari, Jm., Mirfeizi, Sz. (2010)The effects of whortleberry on controlling of blood glucose and lipids in patients with type II diabetes: A randomized controlled trial. Journal of Bioenergetics and Biomembranes. Dec 34(4);321-8.
20.MoeiniFard, M., Hedayati, M. (1393). Alloxan and streptozotocin, diabetes research tool. Applied Sport Physiology Research,10(20); 13-22.
21.Marangella, M., Vitale, C., Bagnis, C., Bruno, M., Ramello, A. (1999). Idiopathic calcium nephrolithiasis. Nephron, 81; 38-44.
22.Palsamy, P., Subramanian, S. (2008). Resveratrol, a natural phytoalexin, normalizes hyperglycemia in streptozotocin-nicotinamide induced experimental diabetic rats. Biomed Pharmacother, 62; 598-605.
23.Papa, S., Skulachev, V. (1997). Reactive oxygen species, mitochondria, apoptosis and aging. Detection of Mitochondrial Diseases, 305-319.
24.Pereira ,AdS., Spagnol, AR., Luciano, E., Leme, JACdA. (2016). Influence of aerobic exercise training on serum markers of oxidative stress in diabetic rats. Journal of Physical Education, 27(3); 20-9.
25.Pérez-Gallardo, RV., Noriega-Cisneros, R., Esquivel-Gutiérrez, E., Calderón-Cortés, E., Cortés-Rojo, C., Manzo-Avalos, S. (2014). Effects of diabetes on oxidative and nitrosative stress in kidney mitochondria from aged rats. Journal of Bioenergetics and Biomembranes, 46(6); 511-8.
26.Ryter, SW., Kim, HP., Hoetzel, A., Park, JW., Nakahira, K., Wang, X. (2007). Mechanisms of cell death in oxidative stress. Antioxidants & Redox Signaling, 9(1); 49-89.
27.Sadi, G., Kartal, Dİ., Güray, T. (2013). Regulation of glutathione S-transferase Mu with type 1 diabetes and its regulation with antioxidants. Turkish Journal of Biochemistry/Turk BiyokimyaDergisi, 1;38(1).
28.Sanders, RA., Rauscher, FM., Watkins, III JB. (2001). Effects of quercetin on antioxidant defense in streptozotocin‐induced diabetic rats. Journal of Biochemical and Molecular Toxicology, 15(3); 143-9.
29.Sha, J., Sui, B., Su, X., Meng, Q., Zhang, C. (2017). Alteration of oxidative stress and inflammatory cytokines induces apoptosis in diabetic nephropathy. Molecular Medicine Reports, 16(5); 7715-23. DOI:
30.Siu, P., Bryner, R., Martyn, J., Always, SE. (2004). Apoptotic adaptations from exercise training in skeletal and cardiac muscles. J Fed Am Soc Exper Biol., 18(1); 1150-1152.8
31.Soleas, G.J., Goldberg, D.M., Grass, L., Josephy, P.D., Diamandis, E.P. (2002). Acomparison of the anticarcinogenic propertities of four red wine polyphenols. Clin. Biochem., 35; 119–124.
32.Smoliga, JM., Baur, JA., Hausenblas, HA. (2011). Resveratrol and health–a comprehensive review of human clinical trials. Molecular Nutrition & Food Research, 55(8); 1129-41.
33.Torres, M., Canal, J., Perez, C. (1999). Oxidative stress in normal and diabetic rats. Physiological Research, 48(3); 203-208.
34.Zhang, H., Zhang, J., Ungvari, Z., Zhang, C. (2009). Resveratrol improves endothelial function: role of TNFα and vascular oxidative stress. Arteriosclerosis, Thrombosis, and Vascular Biology, 29(8); 1164-1171.
_||_