اثر تمرین تناوبی شدید و رژیم غذایی پرپروتئین بر سطوح پلاسمایی آپولیپوپروتئین A1 و B و پروفایل لیپیدی در موشهای صحرایی نر چاق
محورهای موضوعی : فصلنامه زیست شناسی جانوری
مرضیه ثاقب جو
1
,
مهدی علی اکبری
2
,
هانیه حبیبی سنگانی
3
1 - گروه علوم ورزشی، دانشکده علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران
2 - گروه تربیت بدنی و علوم ورزشی، واحد تهران جنوب، دانشگاه آزاد اسلامی، تهران، ایران
3 - گروه علوم ورزشی، دانشکده علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران
کلید واژه: تمرین ورزشی, رژیم غذایی, مقاومت به انسولین, پروفایل لیپیدی, چاقی,
چکیده مقاله :
چاقی با اختلالات لیپیدی و افزایش قابل توجه بیماریهای قلبی- عروقی همراه است. هدف از مطالعه حاضر بررسی اثر 10 هفته تمرین تناوبی شدید (HIIT) و رژیم غذایی پرپروتئین (HPD) بر سطوح پلاسمایی آپولیپوپروتئین A1 و B و پروفایل لیپیدی در موشهای صحرایی نر چاق بود. 40 سر موش صحرایی نر ویستار چاق در پنج گروه هشتتایی: HIIT، HPD، HPD+HIIT، کنترل چاق A (OC-A) و کنترل چاق B (OC-B) و هشت سر موش به‌عنوان گروه کنترل نرمال (NC) مورد مطالعه قرار گرفتند. گروههای تمرین، پنج روز در هفته به مدت 10 هفته با شدت حدود 90 درصد حداکثر اکسیژن مصرفی روی نوارگردان دویدند. پس از پایان مداخله، نمونه‌گیری خون انجام و سطوح آپولیپوپروتئینهای ApoA1، ApoB و پروفایل لیپیدی در پلاسمای حاصل اندازه‌گیری شد. دادهها با استفاده از آزمون آنالیز واریانس یکطرفه و آزمون تعقیبی توکی آنالیز شدند. سطح پلاسمایی HDL-C بهطور معنیداری در گروه NC بالاتر از گروه OC-A بود (05/0 ˂p )، اما بین سایر گروهها تفاوت معنیداری مشاهده نشد (05/0 ˃p ). نسبتهای TC/HDL-C و LDL-C/HDL-C در گروههای NC و HIIT+HPD در مقایسه با گروه OC-A بهطور معنیداری پایینتر بود (05/0 ˂ p)، در حالیکه بین سایر گروهها تفاوت معنیداری مشاهده نشد (05/0 ˃p ). همچنین تری‌گلیسرید-گلوکز (TyG) نیز بهطور معنیداری در گروههای NC، OC-B، HIIT، HPD و HIIT+HPD پایینتر از گروه OC-A بود (05/0p < ). در سطوح پلاسمایی ApoA1، ApoB، ApoB/ApoA1، تری گلیسرید، کلسترول تام، لیپوپروتئین با چگالی پایین و لیپوپروتئین با چگالی بسیار پایین بین گروهها تفاوت معنیداری مشاهده نشد (05/0 ˃p ). بهنظر می‌رسد انجام HIIT همراه با HPD بهواسطه بهبود پروفایل لیپیدی میتوانند از عوارض متابولیک احتمالی ناشی از چاقی و رژیمهای غذایی پرچرب جلوگیری کنند.
Obesity is associated with lipid disorders and a significant increase in cardiovascular diseases. This study aimed to investigate the effect of 10 weeks of high-intensity interval training (HIIT) and high-protein diet (HPD) on the plasma levels of apolipoprotein A1 and B and lipid profile in obese male rats. 40 obese male Wistar rats in five groups (n = 8): HIIT, HPD, HPD+HIIT, obese control-A (OC-A), and obese control-B (OC-B) and eight rats were also studied as normal control group (NC). Training groups, ran five days a week for 10 weeks with at the about intensity of 90% of maximum oxygen uptake on the treadmill. After the end of the intervention, blood sampling was done and the levels of apolipoproteins ApoA1, ApoB, and lipid profile were measured in plasma. The data were analyzed using one-way analysis of variance and Tukey's post-hoc tests at a significance level of p < 0.05. The plasma level of HDL-C was significantly higher in the NC group than in the OC-A group (p ˂ 0.05), but no significant difference was observed between the other groups (p > 0.05). The TC/HDL-C and LDL-C/HDL-C ratios in the NC and HIIT+HPD groups were significantly lower compared with the OC-A group (p ˂ 0.05), while no significant difference was observed between the other groups (p ˃ 0.05). Also, triglyceride-glucose index (TyG) was significantly lower in the NC, OC-B, HIIT, HPD, and HIIT+HPD groups than in the OC-A group (p < 0.05). No significant difference were observed in the plasma levels of ApoA1, ApoB, ApoB/ApoA1, Triglyceride (TG), Total cholesterol (TC), Low-density lipoprotein (LDL-C), and Very low density lipoprotein (vLDL-C) among the groups (p > 0.05). It seems that doing HIIT along with HPD can prevent possible metabolic complications caused by obesity and high-fat diets by improving the lipid profile.
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