Antibacterial activities of green tea extracts on five plant pathogenic bacteria
محورهای موضوعی : Biotechnological Journal of Environmental Microorganismsهادی رهاننده 1 , رویا کریمی پور 2
1 - گروه گیاهپزشکی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران
2 - گروه گیاه پزشکی، واحد رشت ، دانشگاه آزاد اسلامی ، رشت ، ایران
کلید واژه: Solvent, Green tea, Antibacterial, Aqueous extract, Alcoholic extract, Biological control,
چکیده مقاله :
Tea is one of the medicinal plants that is used all over the world. In the preparation of green tea, the leaves are
dried after harvesting and then used without fermentation. Tea extract has antimicrobial properties, but there
is little information about this. The leaves of three clones of 100, seed hybrid and Assam were collected from
tea gardens, and then the collected leaves were dried. Three solvents; aqueous, methanolic and ethanolic, were
used for extraction. The effect of these extracts on the proliferation of five bacteria Clavibacter michiganensis,
Xanthomonas arboricola, Pseudomonas syringae, Dikeya zeae and Pectobacterium carotovorum was investigated
by the paper disc method by measuring the non-growth halo. The results showed that the highest dilutions
of aqueous, ethanolic and methanolic extracts (50%) were the most effective dilutions on the investigated
bacteria. All extracts had the ability to control bacteria. The extracts obtained from clone 100 showed the highest
inhibition rate on the studied bacteria. Among the extracts and clones, the highest amount of inhibition on
Gram-positive bacteria C. michiganensis was by water extract. On average, aqueous extract showed the most
effect and ethanol extract showed the least effect on bacteria. D. zeae showed the highest resistance against
the extracts. It seems that different factors such as type of solvent, concentration, types of microorganisms are
effective in the inhibition rate of green tea extract.
Tea is one of the medicinal plants that is used all over the world. In the preparation of green tea, the leaves are
dried after harvesting and then used without fermentation. Tea extract has antimicrobial properties, but there
is little information about this. The leaves of three clones of 100, seed hybrid and Assam were collected from
tea gardens, and then the collected leaves were dried. Three solvents; aqueous, methanolic and ethanolic, were
used for extraction. The effect of these extracts on the proliferation of five bacteria Clavibacter michiganensis,
Xanthomonas arboricola, Pseudomonas syringae, Dikeya zeae and Pectobacterium carotovorum was investigated
by the paper disc method by measuring the non-growth halo. The results showed that the highest dilutions
of aqueous, ethanolic and methanolic extracts (50%) were the most effective dilutions on the investigated
bacteria. All extracts had the ability to control bacteria. The extracts obtained from clone 100 showed the highest
inhibition rate on the studied bacteria. Among the extracts and clones, the highest amount of inhibition on
Gram-positive bacteria C. michiganensis was by water extract. On average, aqueous extract showed the most
effect and ethanol extract showed the least effect on bacteria. D. zeae showed the highest resistance against
the extracts. It seems that different factors such as type of solvent, concentration, types of microorganisms are
effective in the inhibition rate of green tea extract.
Adil M, Baig MH & Rupasinghe HV (2019) Impact of citral and phloretin, alone and in combination, on major virulence traits of Streptococcus pyogenes. Molecules 24 (23), 4237.
Adil M, Khan R & Rupasinghe HV (2018) Application of medicinal plants as a source for therapeutic agents against Streptococcus pyogenes infections. Curr. Drug Metab. 19 (8), 695–703.
Alstrom S (1992) Antibacterial Activity of Tea and Coffee Wastes against Some Plant Pathogenic Pseudomonas syringae Strains. J. Phytopathology.. 136, 329–334. j.1439-0434.1992.tb01315.x
Archana S & Abraham J (2011) Comparative analysis of antimicrobial activity of leaf extracts from fresh green tea, commercial green tea and black tea on pathogens. J. of Applied Pharmaceutical Science. 01 (08); 149-152.
Baltimore Biological Laboratory (1973) Division of Becton, Dickinson and Company. BBL Manual of Products and Laboratory Procedures. Baltimore.. PP: 84-405.
Chatterjee SK, Bhattacharjee I & Chandra G (2009) In vitro synergistic effect of doxycycline & ofloxacin in combination with ethanolic leaf extract of Vangueria spinosa against four pathogenic bacteria. Indian J. Med. Res. 130 (4), 475.
Chou CL & Chung KT(1999) Antimicrobial activity of tea as affected by the degree of fermentation and manufacturung season. Int. Jour Food Microbial. 48:125-130. 10.1016/S0168-1605(99)00034-3
Cumar A, Arvind CP & Jeyaseelan AT (2012) Built-up and vegetation extraction and density mapping using WorldView-II. Geocarto International. 27(7). 10.1080/10106049.2012.657695
Fakoori H, Rafiei karahroudi Z & Sedaghatfar E (2020) Insecticide effect of Rosmary and Lavander essential oils on adult stage of Callosobruchus maculatus. Bioagrica. 1(1): 1-9.
Fukai K, Ishigami T & Hara Y (1991) Antibacterial Activity of Tea Polyphenols against Phytopathogenic Bacteria. Agricultural and Biological Chemistry. 55, 1895–1897. 10.1080/00021369.1991.10870886
Graham HN (1992) Green tea composition, consumption, and polyphenol chemistry. Preview Medicine Journal. 21: 334-350. 10.100.16091-7435(92)90041-F
. Gupta D, Bhaskar D, Gupta R, Karim B, Jain A & Dalai D (2014) Green tea: a review on its natural anti-oxidant therapy and cariostatic benefits. Biol. Sci. Pharm. Res 2, 8–12.
Kodama S, Yuji I, Atsushi T, Yoko N, Atsushi Y, Satoshi C, Kentaro S, Tomohisa Y, Tomoko K. & Kazuichi H (2008) A Fast and Simple Analysis of Glyphosate in Tea Beverages by Capillary Electrophoresis with On-Line Copper(II)-Glyphosate Complex Formation. J. of Health Science. 52:602-606. 10.1248/jhs.54.602
Minakshi Nl Haseeb A, Imtiaz A, Siddiqui H., Nihal A & Gary S W. (2009) Epigallocatechin-3-gallate (EGCG) sensitizes melanoma cells to interferon induced growth inhibition in a mouse model of human melanoma.Cell cycle. 8(13).
Nataro JP. (2006) A typical enters pathogenic Escherichia coli typical pathogens. Energy. Infect. Dis.. 12: 60-69.
Organization WH (2004) WHO guidelines on safety monitoring of herbal medicines in pharmacovigilance systems: World Health Organization.
Pérez-Burillo S, Navajas-Porras B, López-Maldonado A, Hinojosa-Nogueira D, Pastoriza S & Rufián-Henares JÁ (2021) Green tea and its relation to human gut microbiome. Molecules 26 (13), 3907.
Rahanandeh H & Moshaiedy M.(2014) Potency evaluation of Pseudomonas aeroginosae and Pseudomonas fluorescens as biocontrol agents for root-knot nematodes in Iran - International Journal of Biosciences.5. 147-153. 10.12692/ijb/5.8.147-153
Reygaert WC. (2014) The antimicrobial possibilities of green tea. Frontiers in Microbiology. 5.434.2. doi: 10.3389/fmicb.2014.00434
Sartini S, Natsir DM, Muhammad NA. & Andi DP. (2020) Phenolic-rich green tea extract increases the antibacterial activity of amoxicillin against Staphylococcus aureus by in vitro and ex vivo studies. J. of Pharmacy & Pharmacognosy Research. 8 (6), 491-500.
Sarwa KK, Rudrapal M, Debnath M. (2013) Extraction of green tea leaves: the use of different methods, their optimization and comparative evaluation. Biosci. Biotechnol. Res. Asia 10 (1), 383–386.
Sharma A, Sonal G, Indira PS, Shweta D & Reema G. (2012) Green tea extract: Possible mechanism and antibacterial activity on skin pathogens. Food Chemistry. 135,672-675. 10.1016/j.foodchem.2012.04.143
Sugita K, Amana F, Okubot A & Iwaki M. (1999) Epigallocatechin gallate and gallocatechin gallate in green tea catechins inhabit extra cellular relrase of Vero pesticide from enterohemorr haggis Escherichia coli . 0157: 117. Biochim. Biophys. Acta. 1472: 42-50. 10.1016/s0304-4165(99)00102-6.
Nakamura T, Nakazawa Y, Onizuka S, Satoh S, Chiba A, Sekihashi K & Sasaki YF (1997). Antimutagenicity of Tochu tea (an aqueous extract of Eucommia ulmoides leaves): 1. the clastogen-suppressing effects of Tochu tea in CHO cells and mice. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 388(1), 7-20.
Yang Y & Tong Z. (2019) Antimicrobial Activities of Tea Polyphenol on Phytopathogens. Molecules. 24, 816. 10.3390/molecules24040816
