Evaluation of In Vitro Antifungal Activity of Garlic, Thyme, Aloe Vera, and Cinnamon Essential Oils Against Candida Albicans, Aspergillus Flavus, Aspergillus Niger and Mucor Himalis
محورهای موضوعی : Report of Health Care
Mohammad Hosein Sadeghi Zali
1
(Department of Bacteriology, Veterinary Faculty, Islamic Azad University, Urmia Branch, Urmia, Iran)
Saber Yousefi
2
(Department of Microbiology, Virology and Immunology, Urmia University of Medical Sciences, Urmia, Iran)
Amin Hashempour
3
(Young Researcher and Elites club, Urmia Branch, Islamic Azad University, Urmia, Iran)
Nazereh Hashempour
4
(Young Researcher and Elites club, Urmia Branch, Islamic Azad University, Urmia, Iran)
Saman Seyedgholizadeh
5
(Young Researcher and Elites club, Urmia Branch, Islamic Azad University, Urmia, Iran)
کلید واژه: Candida Albicans, Antifungal Activity, Disk Diffusion Assay, Essential Oils, Garlic,
چکیده مقاله :
Introduction: Antifungal drugs have major limitations such as fungal resistance to classical drugs and treatment costs. It has been reported that antifungal activity of some essential oils as safe combinations. Thus, the present study was conducted to investigate the antifungal activity of garlic, thyme, aloe vera and cinnamon essential oils against Candida albicans, Aspergillus flavus, Aspergillus niger and Mucor himalis. Methods: Antibacterial activities of essential oils were evaluated by disk diffusion assay. The data were collected for 1, 3, 7 and 10 days. Results: Findings indicated that all essential oils showed similar antifungal activity against A. flavus, A. niger and M. himalis. Garlic essential oil was more efficient against C. albicans compared with other essential oils. Conclusion: The selected essential oils can efficiently show antifungal activity against A. flavus, A. niger and M. himalis, but garlic essential oil showed antifungal activity against the selected fungus Thus, garlic essential oil can be suggested as helper for common antifungal drugs.
1. Kauffman CA. Fungal infections. Proc Am Thorac Soc. 2006; 3: 35- 40.
2. Enoch DA, Ludlam HA, Brown NM. Invasive fungal infections: A review of epidemiology and management options. J Med Microbiol. 2006; 55: 809- 818.
3. Cruz MCS, Santos PO, Barbosa AM JR, Mélo DLFM, Alviano CS, Antoniolli AR, Alviano DS, Trindade RC. Antifungal activity of Brazilian medicinal plants involved in popular treatment of mycoses. J Ethnopharmacol. 2007; 111: 409- 412.
4. Singh N. Impact of current transplantation practices on the changing epidemiology of infections in transplant recipients. Lancet Infect Dis. 2003; 3: 156- 161.
5. Eggimann P, Garbino J, Pittet D. Management of candida species infections in critically ill patients. Lancet Infect Dis. 2003; 3: 772- 785.
6. Link HF. Observationes in ordines plantarum naturales. Dissertatio prima complectens Anandrarum ordines Epiphytas, Mucedines, Gastromycos et Fungos. Gesellschaft fur Natur (Berlin). 1809; 3: 3- 42.
7. Denning DW. Invasive aspergillosis. Clin Infect Dis. 1998; 26: 781- 803.
8. Rath PM, Ansorg R. Value of environmental sampling and molecular typing of aspergilli to assess nosocomial sources of aspergillosis. J Hosp Infect. 1997; 37: 47- 53.
9. Diaz-Guerra TM, Mellado E, Cuenca-Estrella M, Gaztelurrutia L, Navarro JIV, Tudela JLR. Genetic similarity among one Aspergillus flavus strain isolated from a patient who underwent heart surgery and two environmental strains obtained from the operating room. J Clin Microbiol. 2000; 38: 2419- 2422.
10. McNeil MM, Nash SL, Hajjeh RA, Phelan MA, Conn LA, Plikaytis BP, Warnock DW. Trends in mortality due to invasive mycotic diseases in the United States, 1980-1997. Clin Infect Dis. 2001; 33: 641- 647.
11. Araiza J, Canseco P, Bonifaz A. Otomycosis: clinical and mycological study of 97 cases. Rev Laryngol Otol Rhinol (Bord). 2006; 127: 251- 254.
12. Loudon KW, Coke AP, Burnie JP, Shaw AJ, Oppenheim BA, Morris CQ. Kitchens as a source of Aspergillus niger infection. J Hosp Infect. 1996; 32: 191- 198.
13. Rapp RP. Changing strategies for the management of invasive fungal infections. Pharmacotherapy. 2004; 24: 4S- 28S.
14. Kalemba D, Kunicka A. Antibacterial and antifungal properties of essential oils. Curr Med Chem. 2003; 10: 813- 829.
15. Pina-Vaz C, Rodrigues AG, Pinto E, Costa-de-Oliveira S, Tavares C, Salgueiro LR, Cavaleiro C, Gonc alves MJ,
Martinez-de-Oliveira J. Antifungal activity of Thymus oils and their major compounds. J Eur Acad Dermatol. 2004; 18: 73- 78.
16. Salgueiro LR, Cavaleiro C, Pinto E, et al. Chemical composition and antifungal activity of the essential oil of Origanum virens on Candida species. Planta Med. 2003; 69: 871- 874.
17. Salgueiro LR, Pinto E, Gonc alves MJ, et al. Chemical composition and antifungal activity of the essential oil of Thymbra capitata. Planta Med. 2004; 70: 572- 575.
18. Blackwood J, Fulder S. Garlic; nature’s original remedy. Javelin Books. 1986.
19. Yusuf KO, Ekanem JT. Studies of phytochemical constituents and antitrypanosomal properties of fermented wheat germ and garlic bulbs extract on Trypanosoma brucei- infected rats. J Med Plants Res. 2010; 4 (19): 2016- 2020.
20. Abu-Darwish MS, Abu-Dieyeh ZH, Batarseh M, Al-Tawaha ARM, Al- Dalian SYA. Trace element contents and essential oil yields from wild thyme plant (Thymus serpyllum L) growth at different natural variable environments. Jordan J Food Agric Environ. 2009; 7: 920- 924.
21. Malterud KE, Fabrot TL, Huse AE. Antioxidant and radical scavenging effects of anthraquinones and anthrones. Pharmacology. 1993; 47 (1): 77- 85.
22. Ramamoorthy L, Tizard IR. Induction of opoptosis in a macrophage cell line RAW 264.7. Molecular Pharm. 1998; 53: 415- 421.
23. Tizard I, Busbee D, Maxwell B, Mc K. Effect of acemannan, a complex carbohydrate, on wound healing in young and aged rats. Wounds. 1994; 6: 201- 209.
24. Ciftci M, Simsek UG, Yuce A, Yilmaz O, Dalkilic B. Effects of dietary antibiotic and cinnamon oil supplementation on antioxidant enzyme activities, cholesterol levels and fatty acid compositions of serum and meat in broiler chickens. Acta Vet Brno. 2010; 79: 33- 40.
25. Lamaty G, Menut C, Bessiere JM, Zollo PHA, Fekam F. Aromatic plants of tropical Central Africa. I. Volatile components of two annonaceae from Cameroon: Xylopia aethiopica (Dunal) A. Richard and Monodora myristica (Gaerth.) Dunal Flavours Fragrance J. 1987; 2: 91- 94.
26. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by standardized single disc method. Am J Clin Pathol. 1996; 44: 493- 496.
27. Baydar H, Sagdic O, Ozkan G, Karadogan T. Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control. 2004; 15: 169- 172.
28. Nostro A, Blanco A R, Cannatelli M A, Enea V, Flamini G, Morelli I, Roccaro A S, Alonzo V. Susceptibility of methicillin-resistant staphylococci to oregano essential oil, carvacrol and thymol. FEMS Microbiol Lett. 2004; 230: 191- 195.
29. Valero M, Salmeron MC. Antibacterial activity of 11 essential oils against Bacillus cereus in tyndallized carrot broth. Int J Food Microbiol. 2003; 85: 73- 81.
30. Dewang PM, Nikumbh VD, Tare VS, Mahulikar PP. Eco-friendly pest management using monoterpenoids II Antifungal efficacy of Menthol Derivatives. J Sci Ind Res. 2003; 62: 990- 995.
31. Rasooli I, Abyaneh M R. Inhibitory effects of Thyme oils on growth and aflatoxin production by Aspergillus parasiticus. Food Control. 2004; 15: 479- 483.
32. Tullio V, Nostro A, Mandras N, Dugo P, Banche G, Cannatelli M A, Cuffini A M. Alonzo V, Carlone NA. Antifungal activity of essential oils against filamentous fungi determined by broth
microdilution and vapour contact methods. J Appl Microbiol. 2007; 102: 1544- 1550.
33. Klaric MS, Kosalec I, Mastelic J, Pieckova E, Pepeljnak S. Antifungal activity of thyme (Thymus vulgaris L.) essential oil and thymol against moulds from damp dwellings. Lett Appl Microbiol. 2007; 44: 36- 42.
34. Cock IE. Antimicrobial activity of Aloe barbadensis Miller leaf gel components. Int J Microbiology. 2008; 4 (2): 1937- 8289.
35. Viuda-Martos M, Ruiz-Navajas Y, Fernández-López J, Pérez-Álvarez J. Antifungal activity of lemon (Citrus lemon L.), mandarin (Citrus reticulata L.), grapefruit (Citrus paradisi L.) and orange (Citrus sinensis L.) essential oils. Food Control. 2008; 19: 1130- 1138.
36. Carson CF, Mee BJ, Riley TV. Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage and salt tolerance assays and electron microscopy. Antimicrobial Agents and Chemotherapy. 2002; 46: 1914- 1920.
37. Conner DE, Beuchat LR. Effect of essential oils from plants on growth of food spoilage yeasts. J Food Sci. 1984; 49: 429- 434.
38. Omidbeygi M, Barzegar M, Hamidi Z, Naghdibadi H. Antifungal activity of thyme, summer savory and clove essential oils against Aspergillus flavus in liquid medium and tomato paste. Food Control. 2007; 18: 1518- 23.
39. Daferera DJ, Ziogas BN, Polissiou MG. GC–MS analysis of essential oils from some Greek aromatic plants and their fungitoxicity on Penicillium digitatum. J Agricultural Food Chem. 2000; 48: 2576- 2581.
40. Sharma N, Tripathi A. Effects of citrus sinensis (L.) Osbeck epecarp essential oil on growth and morphogenesis of Aspergillus niger (L.) Van Tieghem. Microbiological Res. 2006; 163: 337- 344.
41. Beuchat LR, Golden DA. Antimicrobials occurring naturally in foods. Food Tech. 1989; 43: 134- 142.
